MEIJI TECHNO TM200 (01) PDF Document

The MEIJI TECHNO TM200 Series Microscope is usually supplied in a styrofoam container or a wooden cabinet.

From a Styrofoam Container:

1. The objectives are housed in plastic shipping capsules. The eyepiece is locked to the eyepiece tube of the microscope.

2. Remove the microscope from its styrofoam container by grasping the arm of the instrument and supporting the base with the other hand.

From a Wooden Cabinet:

1. Open the lid of the wooden cabinet with a key attached to the cabinet.

2. Remove the microscope from the cabinet by unscrewing the bolt which is fastening the microscope stand to the cabinet through the bottom of the wooden cabinet. A metal plate is attached to the cabinet to use for unscrewing the bolt.

Assembly:

1. Take out the objectives from the compartment located in the wooden cabinet and also the eyepiece from the separate compartment located on the other side of the objectives. If in a styrofoam container, the objectives will be in plastic capsules.

2. Place the microscope on a firm table.

3. Remove the objectives from plastic capsules.

4. Install the objectives in a clockwise direction from the lowest to the highest power. Care should be taken not to drop them or allow your fingers to contact the lens.

1. Select a position to work where little light falls on the instrument. The worst position is to face a large window, because light falling directly on the slide will affect contrast and resolution.

2. If your microscope is equipped with a mirror and fork, place your bench lamp 20-25cm from the microscope. If your microscope is equipped with an illuminator, make sure that the mains voltage shown on the plate sticked onto the microscope matches your mains supply.

3. If your microscope is equipped with a vertical body tube, incline the main body of the microscope by grasping the arm to adjust it to the most comfortable viewing position. If your microscope is equipped with an inclined body tube, you do not need to incline the microscope body as it has the 45° inclined tube. The head can be rotated 360° so that either the conventional position or with the stage nearest the observer can be utilized.

4. Secure the microslide specimen to be observed to the stage under the spring stage clips. If using a mechanical stage, pull back the lever on the left side of the stage, insert the slide, then bring the crescent shaped holder into contact with the slide. Be certain that the coverslip of the slide is facing toward the objective, otherwise you will not be able to focus your specimen at high magnification.

5. Rotate the nosepiece to position the lowest power objective.

1. After setting up the microscope and placing the specimen on the stage, rotate the nosepiece to position the lowest power objective.

2. Focus the objective on your specimen by turning the large coarse adjustment knob until the image of your specimen is bright and clear.

3. Now bring the specimen into sharp focus by turning the slightly smaller fine adjustment knobs.

4. With the specimen now in sharp focus, rotate the nosepiece to the other objectives and focus using the fine adjustment knob. Since the optics on the TM200 microscope are both par-focal and par-centered, only a slight turn of the fine focus knob will be necessary.

NOTE: Because of a built-in stop, the 4X and 10X objectives can never come into contact with your microslides. The 40X(S) and 100X(S) objectives may occasionally touch the microslide, but because these lenses are in retractable mounts, your slide will not be damaged.

The iris diaphragm is not intended to control the brightness of the illumination but induces contrast into the specimen by diffracting light rays. Focusing of the specimen should be done with the iris diaphragm opened to its maximum aperture.

General Diaphragm Adjustment:

If your microscope is equipped with a disc diaphragm, turn the diaphragm so that the number 2 or 3 opening is aligned with the instage condenser, and then adjust the diaphragm until proper specimen contrast is reached.

If your microscope is equipped with a condenser iris diaphragm, open the diaphragm fully with the lever located immediately below the condenser. Initially it is useful to have as much light as possible.

If additional contrast is required to permit accurate viewing of the specimen, the diaphragm should be slowly closed until the details of the specimen are sharply defined. Care should be taken not to use an aperture too small to gain high contrast, as then fine structure of the image will be destroyed.

Correct Focusing of the N.A. 1.25 Condenser:

a) Raise the condenser to its upper limits and open iris diaphragm fully.

b) Focus the specimen with 40X objective. The diameter of the cone of light should fill the back lens of the objective. This utilizes the full resolving power of the objective. However, most specimens react better to a cone of light approximately 3/4 the diameter of the back lens of the objective.

c) Lower the condenser to achieve a cone of light approximately 3/4 the diameter of the lens.

d) If additional contrast is required to permit study of the specimen, the iris diaphragm may be closed slightly.

When you use the 100X objective, it is necessary to exclude air from the space between the cover glass over the specimen and the front lens of the 100X objective. This is accomplished by placing a drop of immersion oil onto the cover glass.

1. Place a drop of immersion oil onto the cover glass of your specimen.

2. Manipulate the focus controls to immerse the front lens of the objective into the oil. This forms an air-tight connection through which the specimen may be viewed without interference from the atmosphere.

3. Be careful not to let the lens of the objective and the cover glass come into direct contact, as this may scratch or otherwise mar the viewing area of the lens itself.

4. Oil immersion objectives should be cleaned immediately after each use since the oil will dry after a time and prevent satisfactory viewing thereafter.

The MEIJI TECHNO TM200 Microscope is a precision instrument and requires only minimum maintenance. With ordinary care, the microscope will last a lifetime.

Cleaning After Use:

Microscopes, like other precision instruments, should be cleaned after each use to prevent dust and other contaminants from drying on exposed surfaces.

Cleaning Lenses:

Eyepiece and objective lenses should never be wiped while dry. Particles of dust should be removed using a soft camel’s hair brush or air. Use lens paper folded several times and moistened with an approved lens cleaner such as xylol or xylene to clean glass surfaces. Lenses should never be disassembled except by qualified, authorized technicians.

Cleaning the Finish:

The finish of the Microscope is hard epoxy and is acid resistant. It is extremely durable and stands up well under rough use. The finish should be wiped off periodically with a soft cotton cloth.

Periodic Servicing:

Periodic servicing is recommended. This should be done only by qualified technicians since general servicing includes disassembly, cleaning, and relubrication. At this time, all parts are tightened and inspected for wear. The period of maintenance will vary depending on the hours of use of the microscope each day. Some schools will find servicing every two years adequate, while others will require more frequent attention.