MEIJI TECHNO MT4200H (01) PDF MANUAL

• Computer Aided Design
• All New and Improved Planachromat and S.Plan Phase Objectives
• Slim Compact Footprint
• All New Infinity Corrected Optical System
• Abbe Condenser with NA 1.25 with Iris in Dovetail Mount
• Low Positioned Ergonomic Coaxial Coarse and Fine Focus Controls
• Smooth Operating Ergonomic Reverse Facing Quintuple Nosepiece
• Ceramic Coated Scratch Resistant Flat Top Stage, left or right-handed
• Brightfield and Phase Contrast Observation Modes Available
• LED Illuminated or Halogen Illuminated models available
• Siedentopf-type Binocular and Trinocular Viewing Heads
• Widefield High Eyepoint Compensating Eyepieces (KHW10X, F.N.20)
• Automatic Voltage Sensing Power Supply with detachable power cord
• Wide Range of Filters and Accessories

Meiji Techno products are designed for safe operation under normal operating conditions. The instrument and accessories described in this manual have been built and tested according to industry safety standards for electronic laboratory instruments. Incorrect usage or non-conformance to operating instructions can cause personal injury or damage to equipment or property. Keep this manual near your instrument for easy reference.

This product is designed and intended for use only as a biological microscope system. Modifying this instrument in any way for use in any situation other than the original and intended product design will automatically void the warranty.

WARNING: DO NOT OPERATE UNLESS THE UNIT IS PROPERLY GROUNDED!

• Use only the specified power cord in a well grounded outlet. Do not use in an ungrounded power outlet or in cases where there is a break in the ground conductor or other damage to electrical wiring.
• Only fuses of the specified type and rating are to be used as replacements. Switch off the power and disconnect the power cord before replacing fuses. Use of a non-compliant fuse may result in electrical shock or severe damage your equipment.
• Do not replace the bulb for at least 10 minutes after the unit has been turned off or injury may result.
• Modifying the instrument in any way or unauthorized attempts to disassemble or use the instrument for applications other than its intended design will automatically void the warranty.

The microscope should be operated in a room with as little dust as practically possible. Keep your instrument away from solvents, chemical fumes and excessive humidity. Also try to avoid big swings in ambient temperature, direct sunlight and vibration as they can affect measurements and instrument performance.
Operating Ambient Conditions:

• Temperature: 10 – 36°C (50 – 96.8°F)
• Relative Humidity: 0 – 80% up to 30°C (86°F)

Please check your packing slip to insure that all materials are present. Keep the packing materials in a safe place for the purpose of storage and transporting the microscope and its accessories.
ATTENTION: Avoid touching the surface of optical components such as lenses, filters and glass surfaces. Even very small traces of perspiration or finger oils can corrode the surfaces of optics in a short period of time.

1. As a first step, remove all components from the shipping container and remove the packing materials. Save the containers and packing in a dry location.
2. Place the microscope frame on a stable work surface.
3. Loosen the clamp screw on the microscope limb and then install the prism house of the viewing head (Fig.1 in PDF) and mount the binocular head to the prism house (Fig.2 in PDF) by tightening the set screw. Re-tighten the clamp screw while the head is in the correct position as shown.
4. Install the two eyepieces (MA407) by sliding them into the head. The eyepieces for MT4000 Series are a DIN and JIS standard 23.2mm diameter. (Fig.3 in PDF)
5. Remove the objectives from their objective cases while being careful not to touch any part of the optics. Then, screw each objective into a nosepiece opening. Install them incrementally or in order of power (e.g. 4, 10, 40, and 100) as shown. (Fig.4 in PDF)
6. Plug the power cord into the microscope and the other end into a grounded outlet. (Fig.5 in PDF)

Note: Your MT4000 has a voltage sensing power supply so it can be used with any voltage from any country in the world from 100 volts to 240 volts AC with the proper cordset.
CAUTION: The mains power cord should only be plugged into a known grounded outlet. Contact your facilities technician if you are unsure of your mains outlet status. A simple outlet tester can be used to verify correct outlet polarity and the presence of a ground circuit.
If no other accessories are going to be installed, the instrument is now ready for use.

The Interpupillary Distance (IP) is the distance between your two pupils.

1. Adjust the IP by pulling apart or pushing together the eyetubes until you see one uniform round field of view (FOV).
2. Note the number marked on the viewing head scale for future reference.
3. Place a specimen on the stage. Close your left eye and focus the image for your right eye using the coarse and fine focus knobs.
4. Close your right eye and open your left eye. Use the diopter adjustment ring on the left eyetube to focus the image for your left eye, without changing the main focus knobs.

The microscope is now adjusted for your interpupillary distance and diopter difference. Other users may need to readjust.

Do not install any bulb in your instrument other than ones designated by Meiji Techno:

Model	Bulb Type	Application
MA326	6V 30W Halogen	For Integrated Illuminator MT4200H, MT4300H, MT4210H, MT4310H

Always disconnect the power cord from the back of the microscope when not being used, or when cleaning your instrument or when making any repairs.
CAUTION: Avoid Dismantling – Never attempt to dismantle the instrument. This will void your warranty and could possibly lead to the instrument no longer performing accurately.

1. Turn on the microscope power switch. Adjust the desired brightness using the variable brightness control knob.
2. Place a specimen on the stage. It’s recommended to start with a specimen that has areas of high and low contrast.
3. Select a lower power objective (e.g., 4X or 10X) by rotating the nosepiece until the objective “clicks” into place. This helps find the area of interest quickly.
4. Use the coarse and fine focus knobs to bring the specimen into focus.

The microscope is now adjusted for basic brightfield viewing for this user. Remember that focusing the eyepieces (diopter adjustment) allows compensation for users who wear corrective lenses. If you wear glasses, you may want to remove the rubber eyeguards.

Koehler illumination provides the best combination of contrast and resolution.

1. Turn on the illuminator. Place your specimen slide on the stage. Rotate the 10X objective into position and focus on the specimen.
2. Move the sub-stage condenser up to its highest position using the condenser focus control.
3. Ensure both the field iris (on the lamp house/base) and the aperture iris (in the sub-stage condenser) are fully open. Close the field iris using its control ring until the edge of the iris diaphragm is just visible at the outer edge of the field of view.
4. Use the condenser focus control to raise or lower the sub-stage condenser until the edge of the field iris diaphragm image appears sharp in the field of view.
5. If the image of the field iris is off-center, use the condenser centering screws to move the iris image until it is concentric with the field of view.
6. With the field iris image centered and sharply focused, open the field iris diaphragm just enough so that its edge disappears outside the field of view.
7. Remove one eyepiece and look into the eyetube. Observe the disc of light at the back focal plane of the objective. Close down the aperture iris (using the lever on the condenser) until it covers approximately one-third of the diameter of the bright disc (leaving about two-thirds visible). Replace the eyepiece.

The microscope is now adjusted for Koehler illumination with the 10X objective. Repeat this procedure (steps 3-7, primarily adjusting the aperture iris in step 7) for each objective to ensure critical illumination.

If normal adjustments are not getting the results you expect, check if these conditions exist:

• Incorrect condenser / objective combination being used
• Incorrect components inadvertently installed
• Dirty or smudged optics

Brightfield Objectives – Planachromat – Infinity Corrected – F = 200mm

Part Number	Description
MA965	Planachromat 4X objective, NA: 0.10, WD = 15.3mm (included)
MA966	Planachromat 10X objective, NA: 0.25, WD = 7.3mm (included)
MA968	Planachromat 40X objective, NA: 0.65, WD = 0.5mm (included)
MA970	Planachromat 100X oil objective, NA: 1.25, WD = 0.14mm (included)

Phase contrast is used for high-contrast images of unstained specimens. MT4000 Phase Contrast models typically come standard with 4X Plan, 10X S.Plan Phase, and 40X S.Plan Phase objectives.

1. Install the phase contrast objectives into the nosepiece.
2. Install the Zernike Phase Condenser: Lower the condenser rack fully using its adjustment knob. Loosen the condenser clamp screw. Slide out the standard Abbe condenser and slide in the Zernike Phase Condenser. Tighten the clamp screw. Raise the condenser. (Fig.1 in PDF)
3. Remove the right-hand eyepiece and insert the provided centering telescope (MA458). (Fig.2 in PDF)
4. Rotate the desired phase objective (e.g., 10X) into position. Rotate the annulus turret in the phase condenser to the corresponding position (e.g., “10”).
5. Focus the centering telescope on the phase annulus rings (you should see a bright ring from the condenser annulus and a darker ring from the objective’s phase plate).
6. If the bright ring and dark ring are not perfectly centered (aligned) as shown in Fig.3 (Aligned) in the PDF, use the knurled annulus adjustment rings/screws on the bottom/sides of the Zernike phase condenser to move the bright condenser annulus ring until it is concentric with the dark objective phase ring. (Fig.4 in PDF)
7. Repeat step 6 for each phase objective and its corresponding condenser annulus setting that you intend to use.
8. Remove the centering telescope and re-install the eyepiece.

The microscope is now properly set for phase contrast observation.
Tip: Phase images often appear best when using a green interference filter (MA861/05, 546nm), as achromatic objectives are typically best corrected for green light.

If normal adjustments are not getting the results you expect, check these:

• Wrong phase annulus being used (condenser annulus number does not match objective magnification).
• Phase annulus not centered correctly.
• Wrong condenser installed (e.g., Brightfield condenser still in place).
• Halos around the outlines of details are optical artifacts which may obscure details of your specimen. This is a known limitation of phase contrast microscopy.
• Since phase annuli limit the numerical aperture of the optical system, image resolution may suffer somewhat compared to brightfield.
• If the specimen being observed is too thick, phase shifting will distort the image details.

Phase Contrast Objectives – S. Planachromat – Infinity Corrected – F = 200mm

Part Number	Description
MA930	S.Plan Phase 10X objective, NA: 0.25, WD = 7.3mm, (included)
MA931	S.Plan Phase 20X objective, NA: 0.40, WD = 5.1mm, (included)
MA932	S.Plan Phase 40X objective, NA: 0.65, WD = 0.72mm, (included)
MA933	S.Plan Phase 100X oil objective, NA: 1.25, WD = 0.14mm, (included)

*(Note: Inclusion varies by specific model, consult your packing list or dealer. The description in the PDF indicates 10X and 40X S.Plan Phase are standard on Phase models, along with a 4X Plan objective.)*

For trinocular models (MT4300L, MT4300H, MT4310L, MT4310H), you need additional adapters:

1. Obtain an optional camera attachment tube (MA150/50 or MA150/60).
2. Obtain the correct T2 Adapter Ring that matches your specific camera body mount (e.g., T2-1 for Canon, T2-5 for Nikon, etc.).
3. Attach the T2 Adapter Ring to your camera body.
4. Screw the camera attachment tube (MA150/50 or MA150/60) onto the T2 Adapter Ring on your camera.
5. Loosen the clamp screw on the microscope’s trinocular port.
6. Insert the camera attachment tube (with camera attached) into the trinocular port.
7. Tighten the clamp screw to secure the camera assembly.
8. Pull out the beamsplitter lever to divert light to the camera port.

You may also need a photo eyepiece (e.g., MA512 2.5X, MA500 3.3X, MA508 5.0X) placed inside the camera attachment tube to project the image onto the camera sensor. Consult accessory documentation for the correct combination.

T2 Camera Adapter Rings:

Code	Camera Brand/Mount
T2-1	Canon
T2-2	Minolta
T2-3	Pentax K
T2-4	Pentax S (threaded)
T2-5	Nikon
T2-6	Olympus
T2-7	Contax, Yashica
T2-8	Konica
T2-9	Canon EOS
T2-10	Minolta Alpha / Maxim 2000

To mount a consumer-grade digital camera to MT4300L, MT4300H, MT4310L, or MT4310H microscopes, an optional camera adapter specific to the camera model is needed. The table below shows some compatible cameras and their corresponding adapter part numbers. Note that these adapters typically attach to the camera’s filter thread and then fit into the trinocular port or over an eyepiece. Consult the specific adapter instructions.

Digital Camera Adapter Chart (Example combinations for MT4000/5000/etc. Series):

Adapter Part No.	Compatible Canon Cameras	Compatible Nikon Cameras	Compatible Olympus Cameras	Compatible Fuji Cameras	Compatible Sony Cameras
MA151/30/43	Powershot G1, G2
MA151/30/41	Powershot G3, G5
MA151/30/31	Powershot G6
MA151/30/51		Coolpix 800, 900, 900S, 950, 990, 995 & 4500
MA151/30/71		Coolpix 5000
MA151/30/57		Coolpix 4300, 885
MA151/30/61			Olympus Camedia C-2000, C-2020, C-3000, C-3030, C-3040, C-3100, C-4040, C-4100, C-5050
MA151/30/63			Olympus Camedia C-700, C-720, C-730, C-740, C-750, C-755, C-760, C-765, C-770
MA151/30/65			Olympus Camedia C-5060, C-7070
MA151/30/81				Fuji Finepix S602, 4900Z, 6900Z, S5000, S7000
MA151/30/91					Sony S70, S75, S85, CD300, CD400, CD500

*(Note: This table is based on the provided PDF. Camera compatibility and adapter availability may change. Always verify with Meiji Techno or an authorized dealer for current information.)*

For trinocular models (MT4300L, MT4300H, MT4310L, MT4310H), the integrated male “C” mount on the trinocular port can be used directly with C-mount cameras.

1. Unscrew and remove the chrome tube (photo tube) from the top of the trinocular port to expose the male C-mount threads.
2. Screw your C-mount camera directly onto these threads.
3. Pull out the beamsplitter lever to direct light to the camera.

To change the magnification seen by the camera, or if using a trinocular head without the integrated C-mount (requiring the photo tube), you can use an optional C-Mount Adapter with a lens. These adapters typically slip over the existing photo tube.

Optional “C” Mounts With Lenses (Slip over existing photo tube):

Part Number	Description
MA151/35/03	“C” Mount Adapter with 0.3X lens
MA151/35/04	“C” Mount Adapter with 0.45X lens
MA151/35/50	“C” Mount Adapter with 0.5X lens
MA151/35/15	“C” Mount Adapter with 1.0X lens
MA151/35/20	“C” Mount Adapter with 0.7X lens
MA151/35/25	“C” Mount Adapter with 2.5X lens

WARNING:

• Disconnect the power cord on your equipment prior to performing cleaning, maintenance or repair.
• Keep electrical components away from moisture or humidity.
• In warm humid climates, take special care to prevent your equipment from exposure to fungal growth by using desiccant in an airtight storage container or by other means.
• Clean the microscope after each use. Keeping your microscope clean will insure its proper operation over its lifetime.

Dust Protection: Be sure to use the supplied dust cover with your microscope after each work session.
Cleaning Overview: Dust, fibers and other debris can cause your field of view to get obstructed so keeping your microscope clean will help the overall quality of your work.

Use a soft brush or lint-free cotton cloth to remove dust and loose particles. Tough dirt can be removed with water and a mild detergent.
CAUTION: NEVER USE ACETONE OR OTHER HARSH CHEMICALS. Painted or plastic surfaces should not be tarnished or etched with cleaning agents that are too powerful. To clean painted surfaces, use a moistened lint-free cotton cloth with mild soapy water.

Use a soft brush or lint-free cotton cloth to remove dust and loose particles.
DO NOT USE ACETONE OR OTHER HARSH CHEMICALS.
Use a moistened lint-free cotton cloth with a solution of mild soapy water.

Use a soft brush or lint-free cotton cloth to remove dust and loose particles. For tough dirt, use a soft lint-free cotton cloth moistened with distilled water. If that fails, try using medical or reagent grade isopropyl alcohol. Wipe lenses immediately after cleaning.

DANGER: Objectives should NEVER be disassembled for cleaning or for any other reason! We do not advise cleaning the inside surfaces of objectives or eyepieces.
Use a soft brush, bellows brush or a soft lint-free cotton cloth to remove dust and loose particles from the front lens. For tough dirt, use a soft lint-free cotton cloth moistened with distilled water. If that fails, carefully try using medical or reagent grade isopropyl alcohol. Wipe lenses immediately. Over time, water and solvents can dissolve optical cements that hold optics together so NEVER soak objectives with ANY type of fluid. For oil immersion objectives (100X), use lens cleaning paper or a soft cloth lightly moistened with lens cleaning solution or isopropyl alcohol to remove oil immediately after use.

Please refer to the previous “Operations” chapters which coincide with the observation mode that you are using (e.g., Brightfield, Phase Contrast). The most common operational problems include the improper positioning of contrast accessories, the improper adjustment of phase annuli or the incorrect condenser installed. If you are unable to obtain the desired image from the microscope, please refer to the corresponding chapters of this manual under the proper operation mode: brightfield, phase contrast, etc.
If you continue to have difficulty, contact Meiji Techno or your authorized dealer. Technical staff are trained to assist with mechanical or electrical issues.
Contact Information:

• MEIJI TECHNO CO., LTD. (Japan)
  • Phone: 049-259-0111
  • Fax: 049-259-0113
  • E-mail: meiji@meijitechno.co.jp
  • Web: http://www.meijitechno.co.jp
• Meiji Techno America
  • Phone: 800.832.0060
  • Fax: 408.970.5054 FAX
  • E-mail: technicalsupport@meijitechno.com
  • Web: http://www.meijitechno.com

Electrical problems can include:

• The lamp on the microscope is not working.
• No voltage is present.

Check the following probable causes:

• Check that all power cords are properly connected to the right spots.
• Make sure power is actually present at the wall outlet.
• Check to see if the fuse is blown.
• For Halogen models: Check if the halogen lamp has blown.

CAUTION: ALWAYS DISCONNECT YOUR EQUIPMENT BEFORE DOING ANY REPAIR.
The mains fuse is located on the back panel of the microscope.
Instructions to replace the mains fuse:

1. Turn the power switch to the off position.
2. Unplug the microscope from the wall outlet.
3. Loosen the fuse cap from the fuse holder (usually requires a flat-head screwdriver or coin to twist).
4. Remove the blown fuse from the fuse cap.
5. Replace the fuse with the CORRECT type and rating:
   • 30W Halogen Systems: MA327 – 3 Amp, 250V, 5 X 20mm
   • LED Illuminated Models: MA929 – 0.5 Amp, 250V, 5 X 20mm
6. Reinstall the fuse holder with the new fuse in place.

DANGER: NEVER USE REPLACEMENT FUSES OF A DIFFERENT RATING.

• Make sure your mains outlet indeed has power.
• Make sure the plug from the lamp power supply (if separate) or the main power cord is firmly plugged into the correct socket on the rear panel and the wall.
• Check to see if the mains fuse has blown (See fuse replacement instructions).
• For Halogen models: Check to see if the halogen lamp has blown (See lamp replacement instructions).

LED Illuminated models should not experience bulb failure very often. If the LED illuminator fails, please contact your dealer or Meiji directly for service.

CAUTION: ALWAYS DISCONNECT YOUR EQUIPMENT BEFORE DOING ANY REPAIR.
HOT! LAMP AND SURROUNDING AREA MAY BE HOT TO THE TOUCH.
Do not touch the glass envelope of the lamp during installation. Keep the protective sleeve or bag of the lamp during installation and remove it right after installation.

1. Switch off the microscope.
2. Disconnect the power cord.
3. Wait until the bulb has cooled sufficiently (at least 10 minutes).
4. Carefully lay the microscope on its side on top of a soft towel or blanket to access the bottom.
5. Remove the bottom illuminator cover screw and lift the cover to remove. (Access may vary slightly depending on exact model configuration, typically a door or plate on the base).
6. Gently pull the defective lamp straight out of its socket.
7. Holding the new lamp (MA326) by its base or using its protective wrapping, insert the pins straight into the socket. Ensure it is pushed in as far as possible to align the filament correctly.
8. Remove any protective wrapping from the new bulb.
9. Reinstall the lamp cover door/plate and secure it with the screw.
10. Carefully return the microscope to its upright position.
11. Reconnect the power cord and switch on the microscope to verify proper illuminator operation.

• Protect your microscope from dust after each use by covering your instrument with the protective dust cover that came with your microscope.
• Store your microscope in a cabinet that has a stable temperature and low humidity.
• If you live in an area that has high humidity, consider storing your microscope in a sealed container along with a desiccant such as silica gel.
• It is also recommended that the objective and eyepieces be stored in a separate air tight container with desiccant, especially in humid environments.

• Whenever the microscope is going to be moved, ship or transport the microscope and the accessories in its original packing, if available. Use ample padding to prevent shock and vibration.
• It is advisable to keep a copy of all necessary information: copy of the original invoice, the instruction manual, etc. included with the microscope when shipping, especially for service or warranty claims.

Filter (Example Part No.)	Type / Name	Application
ND25 (MA858/05)	Neutral Density Filter (25% Transmission)	Neutral Filter or ND Filter. Grey filters or neutral density filters are used to attenuate all frequencies of light equally resulting in preservation of color temperature. The ND25 filter would indicate a reduction of light transmission by 75 percent or a passage of light of 25 percent. Used to reduce brightness without altering color, especially for comfortable viewing or with sensitive cameras.
LB100 (MA856/05)	Light Balancing / Blue Filter	Color Temperature Blue Filter. Often included with Halogen models to make the yellowish light appear whiter (closer to daylight). Suppresses red wavelengths in fluorescence applications. Provides a mired shift of approximately 100.
G533 (MA857/05)	Green Filter (approx. 533nm)	Provides contrast enhancement of complimentary colors (red/magenta) on black & white or tungsten-balanced color transparency films. Often used with Phase Contrast optics as objectives are typically well-corrected for green light.
546nm (MA861/05)	Green Interference Filter	Typically included with phase contrast models. Provides optimal contrast for phase imaging as achromatic and plan objectives are usually spherically corrected for green light (around 546nm).

Power Source	100-230V 50/60Hz
Bulb	• MA326 Halogen bulb 6V 30W (Philips code: 5761), 3100°K at max. intensity • LED/SPI 3W LED bulb (LUMILEDS LXHL-LW3C with connector cable)
Bulb Life	• 100 hours average for halogen bulb • 20,000 hours average for LED bulb
Light Control Method	Voltage control
Normal Environmental Conditions	• Indoor use • Altitude up to 2,000 m • MAINS supply voltage fluctuations up to ±10% of the nominal voltage • Temperature: +10 to 36°C (50 to 96.8°F) • Relative Humidity: 0 to 80% up to 30°C (86°F) • Transient overvoltages typically present on the MAINS supply. Note: The normal level of transient overvoltages is impulse withstand (overvoltage) category II of IEC 60364-4-443. • Applicable RATED POLLUTION degree 2

Weight & Dimension:

• Binocular head type: 390mm (D) x 410mm (H) x 212mm (W), 8.0kg
• Trinocular head type: 390mm (D) x 465mm (H) x 212mm (W), 8.7kg

*(Dimensions are approximate based on the diagram in the PDF)*