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What is the ROCKER TFF-MV cartridge used for and what sample preparation is recommended?
The HansaBioMed TFF Hollow Fibers cartridge can be used for EV purification, separation, and concentration from diluted matrices, such as cell conditioned media, urine, plasma, etc. Before loading, sample preparation is recommended, such as centrifugation, to eliminate large particles and prevent the membrane from clogging.
What are the specifications for the different TFF cartridges available for the ROCKER TFF-MV system?
| TFF-Easy | TFF-MV | TFF-EVs Small | |
| Material | Polysulfone (PSU) | Polysulfone (PSU) | Polyethersulfone (PES) |
| Pore Size | 5 nm | 150 nm | 50 nm |
| Retentate | EVs | Medium & large EVs | > 50 nm EVs, Nanoparticles |
| Permeate | Water and small molecules (< 100 kDa) | Small EVs, proteins, soluble molecules | Water and small molecules (< 250 kDa) |
What are the sterility and storage instructions for the ROCKER TFF-MV cartridge?
Sterility: HansaBioMed TFF cartridges are provided sterile. Once used, please sterilize by Beta-irradiation. Do NOT autoclave.
Storage: Store the cartridge at room temperature.
What are the different labeled parts of the ROCKER TFF-MV cartridge?
| Position | Designation | Position | Designation |
| 1, 1a | Sample Injection Nozzle | 4 | Permeate / Filtrate Nozzle |
| 2 | Flow Valve | 5 | Flow Valve |
| 3 | Permeate / Filtrate Nozzle | 6, 6a | Sample Injection Nozzle |
What is the general manual workflow for using the ROCKER TFF-MV cartridge?
All cartridges are suitable for both manual and mechanical use. The general workflow for manual use is as follows:
Step 1. Sample Injection and Filtration
Step 2. Washing
Step 3. Retentate Recovery
Please refer to the instruction of each cartridge for detailed information.
How do I install the ROCKER TFF-MV cartridge for mechanical use with the Tanfil 100 TFF System?
For installation, operation, and cleaning of the TFF System, please refer to the instruction manual of Tanfil 100.
1. Screw 2 male luer couplers each to the feed and retentate gauge mounting block of the Tanfil 100, TFF System.
2. Remove the caps from the sample injection nozzle (1, 6) of the HansaBioMed TFF cartridge. *Please keep all caps for cartridge storage.
3. Attach the sample injection nozzle (1, 6) of the HansaBioMed TFF cartridge directly to the coupler. *The retentate gauge mounting set and capsule bracket set are adjustable to fit the height of the cartridge. *Keep tubing length as short as possible to reduce hold-up volume.
4. Attach male luer lock-1/8″ hose barb connector(s) to the permeate nozzles (3,4) to collect the filtrate.
5. Rotate the flow valves (2,5) to the indicated position to start processing.
6. Load the sample and start processing. Refer to the Tanfil 100 instruction manual for operation.
What are the recommended flow rate and maximum operating pressure for the ROCKER TFF-MV cartridge?
The recommended flow rate for the HansaBioMed TFF cartridge is 30-40 mL/min.
The maximum operating pressure of the HansaBioMed TFF cartridge is 15 psi (1 bar), do not exceed it.
How should I clean and store the ROCKER TFF-MV cartridge?
The cartridge is reusable. Once the filtration process is ended, please wash the cartridge with the following reagents for several times. After washing with chemicals (NaOH, NaClO, Ethanol), please wash with at least 50 mL of MilliQ water to remove the chemical.
* For cell culture, the cartridge is recommended for single use to keep the sterility and prevent contamination.
| Cleaning Solution | General Wash | Easy Fouling | Hard Fouling |
| MilliQ Water | 0.5N~1N NaOH | 0.05% NaClO |
* Using a 40°C~50°C cleaning solution can enhance the cleaning effect.
* If pyrogenic agents exist, wash the cartridge with 96% Ethanol (5–10 mL) to remove potential pyrogenic agents.
* Storage: Let the cartridge dry at room temperature.
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