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What are the specifications for the ROCKER TFF-EASY and other HansaBioMed TFF cartridges?
The specifications for the HansaBioMed TFF Hollow Fibers cartridges are as follows:
| TFF-Easy | TFF-MV | TFF-EVs Small | |
|---|---|---|---|
| Material | Polysulfone (PSU) | Polysulfone (PSU) | Polyethersulfone (PES) |
| Pore Size | 5 nm | 150 nm | 50 nm |
| Retentate | EVs | Medium & large EVs | > 50 nm EVs, Nanoparticles |
| Permeate | Water and small molecules (< 100 kDa) | Small EVs, proteins, soluble molecules | Water and small molecules (< 250 kDa) |
What sample preparation is recommended before using the ROCKER TFF-EASY cartridge?
Before loading your sample into the ROCKER TFF-EASY cartridge, sample preparation is recommended. This includes procedures such as centrifugation to eliminate large particles and prevent the membrane from clogging.
What are the sterility and storage instructions for the ROCKER TFF-EASY cartridge?
Sterility: The ROCKER TFF-EASY cartridge is provided sterile. Once used, it should be sterilized by Beta-irradiation. Do NOT autoclave the cartridge.
Storage: Store the cartridge at room temperature.
Can you identify the parts of the ROCKER TFF-EASY cartridge?
The parts of the ROCKER TFF-EASY cartridge are identified as follows:
| Position | Designation | Position | Designation |
|---|---|---|---|
| 1, 1a | Sample Injection Nozzle | 4 | Permeate / Filtrate Nozzle |
| 2 | Flow Valve | 5 | Flow Valve |
| 3 | Permeate / Filtrate Nozzle | 6, 6a | Sample Injection Nozzle |
What is the general workflow for manual use of the ROCKER TFF-EASY cartridge?
The general workflow for manual use is shown in three steps. Please refer to the instruction of each cartridge for detailed information.
Step 1. Sample Injection and Filtration
Step 2. Washing
Step 3. Retentate Recovery
How do I install and operate the ROCKER TFF-EASY cartridge for mechanical use with the Tanfil 100 system?
For installation, operation, and cleaning of the TFF System, please refer to the instruction manual of Tanfil 100. The cartridge installation procedure is as follows:
1. Screw 2 male luer couplers each to the feed and retentate gauge mounting block of the Tanfil 100 TFF System.
2. Remove the caps from the sample injection nozzle (1, 6) of the HansaBioMed TFF cartridge. Please keep all caps for cartridge storage.
3. Attach the sample injection nozzle (1, 6) of the cartridge directly to the coupler. The retentate gauge mounting set and capsule bracket set are adjustable to fit the height of the cartridge. Keep tubing length as short as possible to reduce hold-up volume.
4. Attach male luer lock-1/8″ hose barb connector(s) to the permeate nozzles (3, 4) to collect the filtrate.
5. Rotate the flow valves (2, 5) to the position indicated to start processing.
6. Load the sample and start processing. Refer to the Tanfil 100 instruction manual for operation details.
Operating Parameters:
The recommended flow rate for the cartridge is 30-40 mL/min.
The maximum operating pressure of the cartridge is 15 psi (1 bar). Do not exceed it.
How should I clean and store my ROCKER TFF-EASY cartridge after use?
The ROCKER TFF-EASY cartridge is reusable. Once the filtration process is ended, please wash the cartridge with the following reagents for several times. After washing with chemicals (NaOH, NaCIO, Ethanol), please wash with at least 50 mL of MilliQ water to remove the chemical.
| Cleaning Solution | General Wash | Easy Fouling | Hard Fouling |
|---|---|---|---|
| MilliQ Water | 0.5N~1N NaOH | 0.05% NaCIO |
Notes on Cleaning:
• Using a 40°C~50°C cleaning solution will enhance the cleaning effect.
• If pyrogenic agents exist, wash the cartridge with 96% Ethanol (5–10 mL) to remove potential pyrogenic agents.
• For cell culture, the cartridge is recommended for single use to keep the sterility and prevent contamination.
Storage:
Let the cartridge dry at room temperature.
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