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What is the ROCKER TFF-EVS SMALL cartridge used for?
The ROCKER TFF-EVS SMALL cartridge can be used for EV purification, separation, and concentration from diluted matrices, such as cell conditioned media, urine, plasma, etc.
What preparation is recommended before using the ROCKER TFF-EVS SMALL cartridge?
Before loading, sample preparation is recommended, such as centrifugation, to eliminate large particles and prevent the membrane from clogging.
What are the specifications for the different HansaBioMed TFF cartridges, including the ROCKER TFF-EVS SMALL?
| TFF-Easy | TFF-MV | TFF-EVs Small | |
|---|---|---|---|
| Material | Polysulfone (PSU) | Polysulfone (PSU) | Polyethersulfone (PES) |
| Pore Size | 5 nm | 150 nm | 50 nm |
| Retentate | EVs | Medium & large EVs | > 50 nm EVs, Nanoparticles |
| Permeate | Water and small molecules (< 100 kDa) | Small EVs, proteins, soluble molecules | Water and small molecules (< 250 kDa) |
How should I handle the sterility of my ROCKER TFF-EVS SMALL cartridge?
The ROCKER TFF-EVS SMALL cartridges are provided sterile. Once used, please sterilize by Beta-irradiation. Do NOT autoclave.
How should I store a new ROCKER TFF-EVS SMALL cartridge?
Store the ROCKER TFF-EVS SMALL cartridge at room temperature.
Can you identify the parts of the ROCKER TFF-EVS SMALL cartridge?
| Position | Designation | Position | Designation |
|---|---|---|---|
| 1, 1a | Sample Injection Nozzle | 4 | Permeate / Filtrate Nozzle |
| 2 | Flow Valve | 5 | Flow Valve |
| 3 | Permeate / Filtrate Nozzle | 6, 6a | Sample Injection Nozzle |
What are the supported usage methods for the ROCKER TFF-EVS SMALL cartridge?
The ROCKER TFF-EVS SMALL cartridge is suitable for both manual and mechanical use.
What is the general workflow for manual use of the ROCKER TFF-EVS SMALL?
The general workflow is as follows:
Step 1. Sample Injection and Filtration
Step 2. Washing
Step 3. Retentate Recovery
How do I install the ROCKER TFF-EVS SMALL cartridge for mechanical use with a Tanfil 100 system?
1. Screw 2 male luer couplers each to the feed and retentate gauge mounting block of the Tanfil 100, TFF System.
2. Remove the caps from the sample injection nozzles (1, 6) of the HansaBioMed TFF cartridge. Please keep all caps for cartridge storage.
3. Attach the sample injection nozzles (1, 6) of the HansaBioMed TFF cartridge directly to the couplers.
* The retentate gauge mounting set and capsule bracket set are adjustable to fit the height of the cartridge.
* Keep tubing length as short as possible to reduce hold-up volume.
4. Attach male luer lock-1/8″ hose barb connector(s) to the permeate nozzles (3, 4) to collect the filtrate.
5. Rotate the flow valves (2, 5) to the open position to start processing.
6. Load the sample and start processing. Refer to the Tanfil 100 instruction manual for operation details.
What are the recommended operational parameters for the ROCKER TFF-EVS SMALL cartridge during mechanical use?
* The recommended flow rate for the HansaBioMed TFF cartridge is 30-40 mL/min.
* The maximum operating pressure of the HansaBioMed TFF cartridge is 15 psi (1 bar); do not exceed it.
How should I clean and store the ROCKER TFF-EVS SMALL cartridge after use?
The cartridge is reusable. Once the filtration process is ended, wash the cartridge with the following reagents for several times. After washing with chemicals (NaOH, NaClO, Ethanol), please wash with at least 50 mL of MilliQ water to remove the chemical.
For cell culture, the cartridge is recommended for single use to keep the sterility and prevent contamination.
| General Wash | Easy Fouling | Hard Fouling | |
|---|---|---|---|
| Cleaning Solution | MilliQ Water | 0.5N~1N NaOH | 0.05% NaClO |
* Using a 40°C~50°C cleaning solution can enhance the cleaning effect.
* If pyrogenic agents exist, wash the cartridge with 96% Ethanol (5–10 mL) to remove potential pyrogenic agents.
* Storage: Let the cartridge dry at room temperature.
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