MOTIC BA310MET-T (01) PDF DOCUMENT

The MOTIC BA310MET-T is suitable for use in all areas of research and industry for observing opaque material, for example in:

a. Metallography

b. Mineralogy

c. Mechanical engineering

d. Electronics

In addition to bright field observation, the MOTIC BA310MET-T is also used for polarized light observation. It can be installed with a digital camera or video camera for photomicrography.

The main components of the Epi Illuminator are:

• Aperture diaphragm adjustment wheel

• Field diaphragm centering screw

• Field diaphragm adjustment wheel

• Clamp screw

• Analyser

• Main body

• Polariser

• Filter slider

• 50W Halogen lamp house

Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high temperature, high humidity, and where it is difficult to unplug the power supply cord. The recommended operating environment is as follows:

• Indoor use

• Altitude: Max 2000 meters

• Ambient temperature: 5°C to 40°C

• Maximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative humidity at 40°C

• Supply voltage fluctuations: Not to exceed ±10% of the normal voltage.

• Pollution degree: 2 (in according with IEC60664)

• Installation / Overvoltage category: 2 (in according with IEC60664)

• Air pressure of 75kPa to 106kPa

• No hoar frost, dew, percolating water, rain

• The automatic voltage selection works with a broad range of settings. However, always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards. Using the wrong power cord could cause fire or equipment damage.

• In case of using the extension cord, use only a power supply cord with a protective earth (PE) wire.

• In order to prevent electric shock, always turn the power switch on the power supply off before connecting the power cord.

Epi-illuminator Electrical Specifications:

• Input: 115/230V~, 60/50Hz, 200VA

• Bulb: 12V 50W Halogen or 12V 3W LED module

• Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

Transmitted Illumination Electrical Specifications:

• Input: 100-240V~, 80VA, 50-60Hz

• Bulb: 6V 30W Halogen or 6V 3W LED module

• Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

Attaching the stage:

• Lower the substage completely with the coarse focus knob.

• Place the stage on the substage and fix it with the four M4 screws that are attached with the stage.

• The 3″ x 2″ stage comes with a glass plate as standard for BA310Met-T. When a slide glass is used for observation, an optional slideglass holder must be attached in place of the glass plate.

• The 6″ x 4″ stage comes with a glass plate as standard for BA310Met-T and a wafer holder is optional.

Installing Objectives:

• Lower the stage completely. Screw the objectives into the revolving nosepiece so that clockwise rotation of the nosepiece brings the next higher magnification objective into position.

Installing the Condenser (for BA310Met-T):

• Raise the stage by turning the coarse focus knob.

• Completely lower the condenser carrier by turning the condenser focus knob.

• Insert the condenser into the dovetail mount with the aperture scale facing forward towards the user. Secure it with the condenser clamp screw.

• Turn the condenser focus knob to raise the condenser as far as it will go.

Attaching the Eyepiece Tube:

• Loosen the eyepiece clamp screw.

• Insert the dovetail adapter on the eyepiece tube into the dovetail mount on the microscope arm.

• Tighten the eyepiece tube clamp screw to secure the eyepiece tube in place.

Installing Eyepieces:

• Use the same magnification eyepieces for both eyes.

• Insert each eyepiece into the eyepiece sleeve, and tighten the clamp screws.

Attaching the Epi Illuminator:

• Loosen the arms clamp screw. Insert the round dovetail adapter on the Epi illuminator into the dovetail mount on the microscope arm.

• For the best image quality, install the Epi illuminator horizontally.

• Plug the power cord from the lamp house to the outlet on the rear panel of the microscope.

Connecting the Main Power Cord:

• Connect the socket of the power cord to the AC inlet on the rear of the base of the microscope. Plug in the other end of the cord to an AC outlet with a ground conductor.

Filters for Epi illuminator:

• Pull out the slider on the Epi illuminator.

• Place the filter and/or ground glass in the filter holder on the slider and make sure the frosted side of the ground glass is faced to the lamp.

• Push the slider to make sure the filter/ground glass stay in the optical path.

Filters for transmitted illumination:

• Remove the collector cover and place the filter in the filter holder located around the field lens, then screw the cover. Take care that dust, dirt, and fingerprints do not get on the filter and the field lens.

A diffuser is built into the base of the microscope. Other available filters and their functions are:

Illumination Brightness Adjustment:

• Turn the brightness adjustment knob fully counterclockwise to the low brightness position.

• Set the power switch to “I” (ON). The green line control lamp in the switch must light up.

• The halogen lamp 12V/50W in the Epi illuminator must light up.

• When the Brightness adjustment knob is turned clockwise to the high brightness position, the light intensity increases.

Coarse and Fine Focusing:

• Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope stand. The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs.

• One rotation of the fine focus knob moves the stage 0.2mm. The graduation on the fine focus knob is 2 microns.

• Never attempt to rotate the left and right knob while holding the other, or turn the coarse and fine focus knobs further than their limit, as this will damage the focusing mechanism.

Coarse Focus Torque Adjustment:

• To increase the torque, turn the torque adjustment ring located behind the left-hand coarse focus knob in the direction indicated by the arrow. To reduce the torque, turn the ring in the opposite direction.

Coarse Focus Quick Stop:

• With the specimen in focus, turn the handle to fix the coarse focus knob. The stage is now fixed at this position and cannot be raised further. The fine focus knob can still be used to lower the stage.

Beam Splitter Lever (Trinocular models):

• When the lever is pushed in completely, 100% of the light enters the observation tube.

• When the lever is pulled out to the limit, the ratio of light entering the observation tube and phototube will be 20:80.

Interpupillary Distance Adjustment:

• Before adjusting, bring a specimen into focus using the 10x objective.

• Adjust the interpupillary distance so that both the right and left field of view become one.

Diopter Adjustment:

• Using the right eye only and viewing through the right-hand eyepiece, adjust the focus with the microscope’s fine or coarse adjustment until the image is at its sharpest.

• Using the left eye only and viewing through the left-hand eyepiece with its independent diopter-focusing ring, focus until the specimen image is at its sharpest.

• The microscope should now be ready for binocular viewing.

Polariser and Analyser of Epi-illuminator:

• Insert the polariser (marked with “P”) into the front slot of the Epi-illuminator.

• Insert the Analyser (marked with “A”) into the side slot of the Epi-illuminator.

• The Analyser is rotatable, and the color of the specimen with polarization will change when rotating.

Field Diaphragm of Epi-illuminator Centering:

• A clear diaphragm image can be obtained after the specimen is in focus.

• Adjust the aperture diaphragm until the aperture diaphragm is 2/3 of the field, then center the aperture diaphragm via the knurled knob on the top of Epi illuminator.

• Set the aperture diaphragm slightly bigger than the field of view by turning the adjustment wheel.

Using the Aperture Diaphragm of the Epi-illuminator:

• The aperture diaphragm adjusts the numerical aperture (N.A.) of the illuminating system, which decides the resolution, contrast, depth of focus, and brightness. It is recommended that the aperture diaphragm is set at 2/3 of the objective N.A to get the best contrast and image quality. To adjust, use the lever and refer to the scale or observe the diaphragm image in the eyepiece tube.

Centering the Condenser (for transmitted illumination):

• Fully open the field and aperture diaphragms.

• Set the specimen on the stage and focus using the 10X objective.

• Close the field diaphragm to its minimum setting.

• Turn the condenser focus knob to bring the field diaphragm image into focus.

• Adjust the condenser centering screws so the image of the field diaphragm appears at the centre of the field of view.

• Adjust and centre the field diaphragm so that it is just outside the field of view for each magnification change.

Using the Aperture Diaphragm (for transmitted illumination):

• An image with appropriate contrast can be obtained with an aperture setting that is 2/3 of the objective N.A. Adjust the condenser aperture diaphragm ring by referring to the scale or observing the diaphragm image in the eyepiece tube.

Using the Field Diaphragm (for transmitted illumination):

• For normal observation, the diaphragm should be set slightly larger than the field of view to avoid flare and lower contrast. The thickness of the glass slide must be 1.7mm or less for the diaphragm to be focused on the specimen plane.

• To ensure vibration-free operation, set the microscope on a sturdy vibration-free table or a bench with a vibration-proof device.

• Pull the optical path selection lever of the trinocular eyepiece tube all of the way out to the limit; the ratio of light entering the observation tube and phototube will be 20:80.

• For the same total magnification, select a combination of the highest possible objective magnification and lowest possible projection lens magnification to achieve the utmost image definition and contrast.

• To ensure optimal illumination, check the position and centring of the lamp and position of the condenser.

• Select a blue filter for routine application. An additional colour-compensating filter can also be used depending on the colour rendition.

• Adjusting the field diaphragm is important for the purpose of limiting extraneous light that may cause flare and lower the contrast. Stop down the diaphragm to achieve an illuminated area slightly larger than that of the field of view.

• A change of depth of focus, contrast and resolution of image is attainable with an aperture setting that is 2/3 of the objective N.A.

General:

• Do not disassemble the microscope. Disassembly may significantly affect the performance of the instrument, and may result in electric shock or injury and will void the terms of the warranty. Never attempt to dismantle any parts other than described in the manual.

Cleaning Lenses and Filters:

• To clean lens surfaces or filters, first remove dust using an air blower. If dust still persists, use a soft/clean brush or gauze.

• A soft gauze or lens tissue lightly moistened with a mixture of alcohol and ether (ratio: alcohol 3 and ether 7) should only be used to remove grease or fingerprints. Because this mixture is highly flammable, be careful handling it around open flame.

• Use the same alcohol and ether mixture to clean immersion oil from objective lenses.

• Do not use the same area of gauze or lens tissue to wipe more than once.

Cleaning Painted or Plastic Components:

• Do not use organic solvents (thinners, alcohol, ether, etc.). Doing so could result in discolouration or in the peeling of paint.

• For stubborn dirt, moisten a piece of gauze with diluted detergent and wipe clean.

• For plastic components, only moisten a piece of gauze with water and wipe clean.

Disinfecting:

• Follow the standard procedures for your laboratory.

When Not in Use:

• Cover the instrument with the vinyl dust cover and store it in a place low in humidity where mould is not likely to form.

• Store the objectives, eyepieces, and filters in a container or desiccator with a drying agent.

Safety Warning: To avoid potential shock hazard, always set the power switch to “O” (OFF) and disconnect the power cord before replacing the bulb. The bulb, lamp housing, and areas near these will be extremely hot during and right after use. Please replace the bulb with a soft cloth or allow it to cool down before handling.

Replacing the 12V/50W Halogen Bulb (Epi-illuminator):

• Fully loosen the slotted head knurled screw on the back of the lamp house.

• Lift the lamp house to remove.

• Remove the old bulb with a piece of gauze and insert the new bulb’s pins all the way into the pin holes on the lamp socket.

• Fit the lamp house from above and tighten the slotted head knurled screw.

Installing the 12V/3W LED Module (Epi-illuminator):

• This is a Motic patent design to exchange the 12V/50W halogen bulb with a 12V/3W LED module on the same socket directly.

• Fully loosen the slotted head knurled screw on the back of the lamp house and lift it to remove.

• Firmly insert the LED module into the socket pinholes until it reaches the limit.

• After installation, secure it with the clamp screw by a 1.5mm hexagonal screwdriver supplied with the microscope.

Replacing the 6V/30W Halogen Bulb (Transmitted Illumination):

• Place the microscope on its back and pull back the lamp house cover plate.

• Firmly insert the new bulb into the socket pinholes until it reaches the limit. Be careful not to tilt the bulb when mounting.

• Do not touch the glass surface of the bulb with bare fingers to avoid leaving fingerprints or grease. If the surface is contaminated, wipe it clean with lens tissue.

• Close the lamp house cover plate and secure it until it snaps into position.

Installing the 6V/3W LED Module (Transmitted Illumination):

• Place the microscope on its back and pull back the lamp house cover plate.

• Firmly insert the LED module into the socket pinholes until it reaches the limit.

• Secure it with the clamp screw by a 1.5mm hexagonal screwdriver supplied with the microscope.

• Close the lamp house cover plate and secure until it snaps into position.