MOTIC BA310MET (01) PDF DOCUMENT

The MOTIC BA310MET and BA310Met-T are suitable for use in all areas of research and industry for observing opaque material, for example, in:

a. Metallography

b. Mineralogy

c. Mechanical engineering

d. Electronics

In addition to bright field observation, the MOTIC BA310MET models are also used for polarized light observation and can be installed with a digital or video camera for photomicrography.

To ensure proper operation, avoid placing the MOTIC BA310MET in locations exposed to direct sunlight, dust, vibration, high temperature, high humidity, and where it is difficult to unplug the power supply cord. The specific operating environment conditions are:

• Indoor use

• Altitude: Max 2000 meters

• Ambient temperature: 5°C to 40°C

• Maximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative humidity at 40°C

• Supply voltage fluctuations: Not to exceed ±10% of the normal voltage.

• Pollution degree: 2 (in according with IEC60664)

• Installation / Overvoltage category: 2 (in according with IEC60664)

• Air pressure of 75kPa to 106kPa

• No hoar frost, dew, percolating water, rain

The MOTIC BA310MET features automatic voltage selection that works with a broad range of settings. However, follow these safety precautions:

• Always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards. Using the wrong power cord could cause fire or equipment damage.

• If using an extension cord, use only a power supply cord with a protective earth (PE) wire.

• To prevent electric shock, always turn the power switch on the power supply off before connecting the power cord.

Epi-illuminator Electrical Specifications:

Input: 115/230V~, 60/50Hz, 200VA

Bulb: 12V 50W Halogen or 12V 3W LED module

Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

Transmitted Illumination Electrical Specifications:

Input: 100-240V~, 80VA, 50-60Hz

Bulb: 6V 30W Halogen or 6V 3W LED module

Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)

Halogen lamp:

The quartz halogen lamp, used as a light source, has higher luminance and color temperature than conventional tungsten lamps. The luminance is approximately four times greater. As long as the lamp voltage is kept constant, the halogen lamp maintains the same level of brightness and color temperature regardless of whether it is new or nearing the end of its life.

LED module:

The LED module is specially designed to be inserted into the halogen bulb socket directly, converting halogen illumination to LED illumination. LED is more economical and environmentally friendly and combines the advantages of low heat and long life span.

To attach the stage:

1. Lower the substage completely with the coarse focus knob.

2. Place the stage on the substage and fix it with the four M4 screws that are attached with the stage.

The 3″ x 2″ stage comes with a glass plate as standard for BA310Met. The 6″ x 4″ stage comes with a glass plate as standard for BA310Met-T. When using a slide glass for observation, an optional slideglass holder must be attached in place of the glass plate.

First, lower the stage completely. Then, screw the objectives into the revolving nosepiece so that a clockwise rotation of the nosepiece brings the next higher magnification objective into position.

Follow these steps to install the condenser for the MOTIC BA310MET-T:

1. Raise the stage by turning the coarse focus knob.

2. Completely lower the condenser carrier by turning the condenser focus knob.

3. Insert the condenser into the dovetail mount with the aperture scale facing forward towards the user.

4. Secure it with the condenser clamp screw.

5. Turn the condenser focus knob to raise the condenser as far as it will go.

Installing the Eyepiece Tube:

1. Loosen the eyepiece clamp screw.

2. Insert the dovetail adapter on the eyepiece tube into the dovetail mount on the microscope arm.

3. Tighten the eyepiece tube clamp screw to secure the eyepiece tube in place.

Installing the Eyepieces:

1. Use the same magnification eyepieces for both eyes.

2. Insert each eyepiece into the eyepiece sleeve, and tighten the clamp screws.

1. Loosen the arm’s clamp screw.

2. Insert the round dovetail adapter on the Epi illuminator into the dovetail mount on the microscope arm. For the best image quality, install the Epi illuminator horizontally.

3. Plug the power cord from the lamp house into the outlet on the rear panel of the microscope.

1. Pull out the slider on the Epi illuminator.

2. Place the filter and/or ground glass in the filter holder on the slider. Ensure the frosted side of the ground glass is faced towards the lamp.

3. Push the slider in to make sure the filter/ground glass stays in the optical path.

Remove the collector cover and place the filter in the filter holder located around the field lens, then screw the cover back on. Take care that dust, dirt, and fingerprints do not get on the filter and the field lens. A diffuser is built into the base of the microscope.

Connect the socket of the power cord to the AC inlet on the rear of the base of the microscope. Plug the other end of the cord to an AC outlet with a ground conductor.

1. Turn the brightness adjustment knob on the MRL100 Power Unit fully counterclockwise to the low brightness position.

2. Set the power switch to “I” (ON). The green line control lamp in the switch should light up.

3. The halogen lamp 12V/50 W in the Epi illuminator should light up.

4. When the Brightness adjustment knob is turned clockwise to the high brightness position, the light intensity increases.

Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope stand. The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs. One rotation of the fine focus knob moves the stage 0.2mm. The graduation on the fine focus knob is 2 microns.

Never attempt either of the following actions, since doing so will damage the focusing mechanism:

• Rotate the left and right knob while holding the other.

• Turning the coarse and fine focus knobs further than their limit.

The coarse focus torque adjustment ring is located behind the left-hand coarse focus knob.

• To increase the torque, turn the torque adjustment ring in the direction indicated by the arrow.

• To reduce the torque, turn the ring in the direction opposite to that indicated by the arrow.

The coarse focus quick stop allows the stage to be fixed at any position where the specimen is in focus.

• With the specimen in focus, turn the handle to fix the knob.

• When the coarse focus quick stop is in position, the stage cannot be raised from that position.

• However, the fine focus knob can still move the stage, but it will only lower the stage from that set position.

• To move the stage up again, you must first disengage the quick stop and use the coarse focus knob.

The beam splitter lever on the trinocular eyepiece tube selects the amount of light distributed between the eyepiece tube and the vertical phototube.

• When the lever is pushed in until it reaches the limit, 100% of the light enters the observation tube (eyepieces).

• When the lever is pulled out to the limit, the ratio of light entering the observation tube and phototube will be 20:80.

1. Before adjusting, bring a specimen into focus using the 10x objective.

2. Adjust the interpupillary distance by moving the eyepiece tubes closer together or further apart until both the right and left field of view merge into one.

3. This adjustment will enable you to observe the specimen with both eyes comfortably.

Diopter adjustment compensates for differences in vision between your eyes. The left eyepiece has a separate focusing provision (diopter-focusing ring) for this.

1. Using your right eye only and viewing through the right-hand eyepiece, adjust the focus with the microscope’s fine or coarse adjustment until the image of the specimen is at its sharpest.

2. Using your left eye only and viewing through the left-hand eyepiece, use its independent diopter-focusing ring to focus until the specimen image is at its sharpest. Do not use the main focus knobs for this step.

3. The MOTIC BA310MET should now be ready for binocular viewing, and the image should remain in focus when changing objective magnifications.

• Insert the polariser (marked with “P”) into the front slot of the Epi-illuminator.

• Insert the Analyser (marked with “A”) into the side slot of the Epi-illuminator.

• The analyser is rotatable. The color of the specimen with polarization will change when the analyser is rotated.

1. A clear diaphragm image can be obtained after the specimen is in focus.

2. Adjust the aperture diaphragm until it is 2/3 of the field of view, then center the aperture diaphragm via the knurled knob on the top of the Epi illuminator.

3. Set the aperture diaphragm slightly bigger than the field of view by turning the adjustment wheel.

The condenser aperture diaphragm adjusts the numerical aperture (N.A.) of the illuminating system, which affects image resolution, contrast, depth of focus, and brightness. Stopping down the diaphragm will lower resolution and brightness but increase contrast and depth of focus. It is recommended that the aperture diaphragm is set at 2/3 of the objective N.A to get the best contrast and image quality.

To adjust the aperture diaphragm:

Adjust the condenser aperture diaphragm lever by referring to the condenser aperture scale, or by observing the diaphragm image visible on the exit pupil inside the eyepiece tube. You can also use a centering telescope after removing one of the eyepieces and focusing on the aperture diaphragm.

1. Fully open the field of view diaphragm and condenser aperture diaphragm.

2. Set the specimen on the stage with the cover glass facing up.

3. Bring the specimen image into focus, using the 10X objective.

4. Close the field of view diaphragm to its minimum setting by means of the field diaphragm ring.

5. Turn the condenser focus knob to bring the field diaphragm image into focus on the specimen plane.

6. Adjust the condenser centering screws so that the image of the field diaphragm appears at the centre of the field of view. At this time, stopping the field diaphragm image just short of the maximum field of view may be convenient for centering.

7. Adjust and centre the field diaphragm so that it is just outside the field of view for each magnification change.

The condenser aperture diaphragm is for adjusting the numerical aperture (N.A.) of the illuminating system, which determines the resolution, contrast, depth of focus, and brightness of the image. Stopping down will lower resolution and brightness but increase contrast and depth of focus. An image with appropriate contrast can be obtained with an aperture setting that is 2/3 of the objective N.A.

To adjust the aperture diaphragm:

• Adjust the condenser aperture diaphragm ring referring to the condenser aperture scale, or

• by observing the diaphragm image visible on the exit pupil inside the eyepiece tube, or

• by using a centering telescope after removing one of the eyepieces and focusing on the aperture diaphragm.

• The field diaphragm determines the illuminated area on the specimen. For normal observation, set the diaphragm slightly larger than the field of view. If set much larger, extraneous light will enter, creating flare and lowering contrast.

• The thickness of the glass slide must be 1.7mm or less, otherwise the field diaphragm may not be focused on the specimen plane.

• The diaphragm has no effect when the condenser top lens is swung out of the optical path (in Swing-out type condensers). In this case, fully open the field diaphragm, as the N.A. of the illuminating system will be reduced if the diaphragm is excessively stopped down.

• A blue filter is used for color temperature adjustment in routine microscopy and photomicrography.

• A frost filter reduces irregularity in the illumination field, but also reduces brightness. To ensure enough brightness, remove the frost filter from the light path when using high magnification objectives and low reflectivity samples.

• For the best contrast and image quality, adjust the condenser aperture diaphragm lever accordingly when the objective is changed.

• To ensure vibration-free operation, set the microscope on a sturdy vibration-free table or a bench with a vibration-proof device.

• Pull the optical path selection lever of the trinocular eyepiece tube all the way out to the limit; the ratio of light entering the observation tube and phototube will be 20:80.

• For the same total magnification, select a combination of the highest possible objective magnification and lowest possible projection lens magnification to achieve the utmost image definition and contrast.

• To ensure optimal illumination, check the position and centring of the lamp and position of the condenser.

• Select a blue filter for routine application. An additional colour-compensating filter can also be used depending on the colour rendition.

• Adjusting the field diaphragm is important for limiting extraneous light that may cause flare and lower the contrast. Stop down the diaphragm to achieve an illuminated area slightly larger than that of the field of view.

• A change of depth of focus, contrast, and resolution of image is attainable with an aperture setting that is 2/3 of the objective N.A.

No. Disassembly may significantly affect the performance of the instrument, may result in electric shock or injury, and will void the terms of the warranty. Never attempt to dismantle any parts other than described in the instruction manual. If you notice any malfunction, contact your nearest Motic representative.

• To clean lens surfaces or filters, first remove dust using an air blower. If dust still persists, use a soft/clean brush or gauze.

• A soft gauze or lens tissue lightly moistened with a mixture of alcohol and ether (ratio: alcohol 3 and ether 7) should only be used to remove grease or fingerprints.

• Use the same mixture of alcohol and ether to clean immersion oil from objective lenses.

• Because the mixture of alcohol and ether is highly flammable, be careful handling it around open flame.

• Do not use the same area of gauze or lens tissue to wipe more than once.

• Do not use organic solvents (thinners, alcohol, ether, etc.). Doing so could result in discolouration or in the peeling of paint.

• For stubborn dirt, moisten a piece of gauze with diluted detergent and wipe clean.

• For plastic components, only moisten a piece of gauze with water and wipe clean.

• To avoid potential shock hazard, always set the power switch to “O” (OFF) and disconnect the power cord before replacing the bulb.

• The bulb, lamp housing, and areas near these will be extremely hot during and right after use. Please replace the bulb with a soft cloth or allow it to cool down completely before handling.

The applicable halogen bulb for the Epi-illuminator is the 12V/50W halogen long-life bulb.

1. Fully loosen the slotted head knurled screw on the back of the lamp house.

2. Lift the lamp house to remove it.

3. Remove the old bulb with a piece of gauze and insert the new bulb’s pins all the way into the pin holes on the lamp socket.

4. Fit the lamp house from above and tighten the slotted head knurled screw.

This is a Motic patent design to exchange a 12V/3W LED module and 12V/50W halogen bulb on the same socket directly.

1. Fully loosen the slotted head knurled screw on the back of the lamp house.

2. Lift the lamp house to remove.

3. Firmly insert the LED module into the socket pinholes until it reaches the limit.

4. After the LED module installation, secure it with the clamp screw by a 1.5mm hexagonal screwdriver supplied with the microscope.

The applicable halogen bulb for transmitted illumination is the 6V/30W halogen long-life bulb.

1. To prevent electric shock, always turn the power switch off and unplug the power cord before installing or replacing the bulb.

2. Place the microscope on its back and pull back the lamp house cover plate.

3. Firmly insert the bulb into the socket pinholes until it reaches the limit. Be careful not to tilt the bulb when mounting.

4. When installing the bulb, do not touch the glass surface of the bulb with bare fingers. Doing so will cause fingerprints, grease, etc., to burn onto the lamp surface, reducing the illumination. If the surface is contaminated, wipe it clean using lens tissue.

5. Close the lamp house cover plate and secure it until it snaps into position.

This is a Motic patent design to exchange an LED module and halogen bulb on the same socket directly.

1. To prevent electric shock, always turn the power switch off and unplug the power cord before installing or replacing the bulb.

2. Place the microscope on its back and pull back the lamp house cover plate.

3. Firmly insert the LED module into the socket pinholes until it reaches the limit.

4. After the LED module installation, secure it with the clamp screw by a 1.5mm hexagonal screwdriver supplied with the microscope.

5. Close the lamp house cover plate and secure it until it snaps into position.

Follow the standard procedures for your laboratory to disinfect the MOTIC BA310MET.

• When not in use, cover the instrument with the vinyl dust cover and store it in a place low in humidity where mould is not likely to form.

• Store the objectives, eyepieces, and filters in a container or desiccator with a drying agent.

Proper handling of the microscope will ensure years of trouble-free service. If repair becomes necessary, please contact your Motic agency or our Technical Service direct.

• If equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

• To avoid getting wet, do not use the microscope near water.