METTLER TOLEDO RAININ MICROPRO MP 1250 (01) PDF MANUAL

The Status LED indicates the instrument’s connection status:

Blue: Instrument is on, and connected to a tablet.

Red: Instrument is on, but NOT connected to a tablet.

Flashing Red: Instrument is on, NOT connected to a tablet, and has gone into Power Save Mode.

Flashing Red and Blue: Instrument is on, but NOT connected to the correct power supply.

Loosen and remove the Nest Thumb Nut to convert the nest from 96 to 384 configuration, and vice versa. Refer to Section 4.7 for more details.

The instrument and tablet communicate wirelessly via a Bluetooth® connection, controlled through the MicroPro Controller app.

The application itself can be password protected. For higher security, standard tablet security features can be used to disable outside connections like WiFi or add password protection to the tablet. Ensure Bluetooth remains enabled for instrument connectivity.

Read all safety warnings and precautions prior to installing, operating or servicing the instrument. Always operate the MicroPro in accordance with the User Manual.

The system is for indoor use only and should be operated by qualified and appropriately trained personnel who have read the operating manual.

Keep control over the pipette head while lifting or moving the instrument, as it moves easily and could present a pinching hazard.

Only use the instrument for its intended purpose (semi-automated liquid transfer for research, development, training, education). Other uses may create hazardous conditions or damage.

Use only Rainin-approved accessories and spare parts to avoid hazardous conditions or damage.

After unpacking, check the box to ensure all the following items are present and there are no signs of shipping damage:

1. MicroPro 20, 300 or 1250 Instrument

2. Power supply and cable

3. Tablet + stand with pre-loaded MicroPro Controller app

4. Rack of tips (for shipping only)

5. Velcro strap

6. Clear polybag

If any items are damaged or missing, contact Rainin or your local Mettler Toledo office.

Carefully remove all items. Keep the box and packaging for transporting the instrument or for authorized service returns.

1. Choose an ideal workspace: a clean, dry bench near an electrical outlet, protected from vibration, drafts, and direct sunlight.

2. Set the instrument on the bench and remove the clear polybag and Velcro strap.

3. Plug in the provided power supply. The power supply is a small box with a cord on each end.

– Plug the round end into the back of the instrument.

– Plug the standard power plug into the electrical outlet.

4. Once plugged in, the instrument will automatically power up.

NOTE: The rack of tips used for shipping purposes can be ejected and discarded.

WARNING: To reduce the risk of electric shock, only use the power supply and cord provided. Do not modify the power supply or cord in any way. Ensure that the power supply, cord, and connector on the instrument are undamaged prior to use.

1. Read the user manual provided by the tablet manufacturer and follow the instructions for powering on and setting up the tablet.

2. It is recommended to fully charge the tablet prior to the first use. If possible, keep it plugged in during use to ensure no interruptions.

3. The MicroPro Controller app will already be pre-installed on the tablet. The app icon will be on the main screen upon startup.

4. To get started, simply press on the MicroPro Controller app icon to open the user interface.

When starting up, your tablet will try to connect to the last pipettor it was connected to by default. If it is unable to find that particular instrument or if you press the Cancel button, the screen will show a list of all available pipettors within range of the wireless connection. Select the desired instrument from the list and choose “Connect”. Alternatively, you can choose “Use Simulator”.

If you choose to use the simulator, all features of the app will still be available. You will be able to create, modify, and save programs as you normally would. While in Simulation Mode, the RUN button will always be available on the main screen, allowing you to dry-run any program without needing to be connected to an instrument.

Note that there is a version of the simulator equivalent to each MicroPro model number. You will need to select the desired version of the simulator that you wish to use.

The Program Graphical Area shows all steps of the program. The active step is highlighted in blue. To edit a specific step, simply click anywhere on that step, and a pop-up will appear. This pop-up will allow you to edit all the parameters of that step. Depending on the mode, various other options may be available, such as Add Mix, Add Dispense, Edit All, etc.

Custom: Shown as a placeholder during the creation of a custom program. Once the step type has been defined, this icon will be replaced by the appropriate symbol.

Aspirate: Represents a normal aspirate step.

Aspirate with Turnaround (Reverse Pipette): Similar to a normal aspirate step, but a small extra amount of liquid is added to the desired volume. Used when there are multiple dispenses to ensure accuracy.

Dispense: Represents a normal dispense step.

Dispense All: Similar to a normal dispense step, but the operation continues through the end of the dispense, purging any remaining liquid that may be in the tips.

Mix: Represents a normal mix step.

Instructions: Found only under custom programs, this step is used to display instructions for the user on the screen during the program.

Purge: Represents a purge step, where all liquid will be cleared from the tips.

Barcode: Allows the user to capture and record a barcode using a Bluetooth-enabled scanner. Limited validation of the barcode can also be configured.

Tip Change: Represents a normal tip eject and/or load cycle. This step type is only available in custom programs.

Manual: Used to manually aspirate and dispense liquid in defined increments that are adjustable while running a program.

Across the bottom of the screen are the Lower Selection Tabs:

Program Name Area: Shows the name of the current program. An asterisk (*) appears if edited but not saved. Pressing this area takes you to a filtered view of the Favorites section for programs of the same type.

New Program / Purge Tab: If a program is being edited and not running, “New Program” is displayed. Pressing it clears the current program (after a warning). If a program has started, “Purge” is displayed. Pressing it cancels the program and purges liquid from tips (after a warning).

Save Program Tab: Saves the current program.

Help Tab: Opens the complete user manual with clickable links.

Settings Tab: Opens the Settings section.

Located on the left side of the screen, the Instrument Status Image shows the current status of the unit and actively updates during program execution.

Instrument Name: Displayed on the head of the pipettor image.

Liquid Level: Shown on the left in the tips, filling up with blue liquid in the image.

Container Name: At the bottom of the image, shows the container corresponding to the active step. Blank if no container is selected.

EJECT Button: To the right of the image, next to the tips.

RUN Button Color: Matches the model unit you are using (orange/yellow for MPR300, red for MPR20, gray for MPR1250 in the app).

The Pipette with Mix function allows a single aspirate followed by a dispense, with or without a mix. To set up:

1. Edit Aspirate Step: Press the Aspirate step. In the pop-up:

– Enter the desired volume in µL using the keypad.

– Select the source container (pre-existing, none, or create new).

– Select the speed (1-10).

– Optionally, select a liquid class.

– Press OK.

2. Edit Dispense Step: Press the Dispense step. In the pop-up:

– The volume will be carried over from the aspirate step (editing it here updates both).

– Select the destination container.

– Select the speed.

– Press OK.

3. Add Mix Step (Optional): Press “Add Mix” located below the dispense step. In the pop-up:

– Edit the mix volume.

– Edit the total number of mixes.

– Edit the speed.

– Press OK.

(To remove a mix step, change the number of mixes to zero and press OK).

To run the program, follow the general steps for running a simple pipette program.

1. Verify that the appropriate tips are loaded.

2. Ensure the selected aspirate container is either in the nest or in the reservoir area.

3. Grab the handle and slowly lower the head until it is at the desired height or all the way to the Z-Stop if you are using a saved container.

4. Press the RUN button on the front of the instrument to aspirate.

5. Raise the head up so that the tips are out of the liquid and you have room to change the container.

6. Ensure the selected dispense container is either in the nest or in the reservoir area.

7. Grab the handle and slowly lower the head until it is at the desired height or all the way to the Z-Stop if you are using a saved container.

8. Press the RUN button on the front of the base unit to dispense.

9. Raise the head up, so that the tips are out of the liquid. Then press the RUN button to return as directed.

CAUTION: The tips must be out of the liquid otherwise you risk sucking liquid back up into the tips. A pop-up window will state: “Remove Tips from Liquid then Press Run to Continue”.

The Repeat Dispense function allows a single aspirate, then dispensing a series of equal amounts. The default is two dispense steps.

1. Edit Aspirate Step: Press the Aspirate step. In the pop-up:

– Select the source container.

– Edit the speed.

– The aspirate volume is calculated directly from the dispense values and is not editable here.

– Press OK.

2. Edit Dispense Steps: Press any repeat dispense step. In the pop-up:

– Edit the repeated volume for each dispense.

– Select the destination container.

– Edit the speed.

– Choose how many repeat dispenses (up to 15, may be restricted by instrument volume/model).

– Press OK.

3. Add/Remove Dispense Steps: Swipe one of the dispense steps to the left. This reveals a green DUPLICATE button and a red REMOVE button. Press DUPLICATE to add a copy below, or REMOVE to delete. (Note: If only two steps, only DUPLICATE is available; a minimum of two dispenses is required).

Running a Repeat Dispense program involves multiple dispense steps of equal volume, followed by a Purge step to remove any extra aspirated volume for accuracy.

The Variable Dispense function allows a single aspiration, then dispensing a series of variable amounts. The default is two dispense steps.

1. Edit Aspirate Step: Press the Aspirate step. The pop-up and options are the same as for Repeat Dispense. The aspiration volume is calculated based on the dispense volumes.

2. Edit Dispense Steps: Press a Dispense step. A standard Dispense edit pop-up appears. Each dispense step adds to the total aspirate volume.

3. Add More Dispense Steps: Press the “+ Dispense” row. A dispense pop-up appears. The system automatically calculates the total volume and restricts dispense steps if they exceed the instrument’s available volume.

4. Add/Remove Dispense Steps: Swipe one of the dispense steps to the left to reveal DUPLICATE and REMOVE buttons.

5. Edit All Dispense Steps (Optional): If enabled in Settings (Pipetting > “Allow Edit All”), press the “Edit All” row to modify all dispense steps simultaneously.

Running a Variable Dispense program is similar to Repeat Dispense, ending with a Purge step.

The Dilute with Mix function involves two aspirates with an air gap in between, followed by a single dispense, with or without a mix.

1. Edit First/Third Aspirate Step: Press either the first or third aspirate step. A pop-up identical to the standard simple pipette function will appear. Set volume, container, and speed.

2. Edit Second Aspirate Step (Air Gap): Press the second aspirate step. The container will be pre-defined as “none” and cannot be changed. Set volume and speed for the air gap.

3. Edit Dispense/Add Mix Step: Press the dispense or add mix step. Pop-up windows identical to the simple Pipette with Mix function will appear, except the dispense volume is not editable as it’s calculated automatically from the total aspirate volume.

When running, ensure tips are out of any liquid when aspirating the Air Gap. The dispense and mix steps are identical to a simple Pipette program.

The Multi-Aspirate function allows multiple aspiration steps, then a single dispense with or without mix.

1. Edit Aspirate Steps: Press any aspirate step. A pop-up identical to those in the Simple Pipette with Mix program appears. The default is two aspirate steps.

2. Add More Aspirate Steps: Press the “+ Aspirate” row to add more aspiration steps.

3. Add/Remove Aspirate Steps: Swipe an aspirate step to the left to reveal DUPLICATE and REMOVE buttons. (Note: If only two steps, only DUPLICATE is available; a minimum of two aspirates is required).

4. Edit Dispense/Add Mix Step: Press the Dispense or Add Mix step. Pop-up windows identical to the simple Pipette with Mix function will appear, but the dispense volume is not editable (calculated from total aspirate volume).

5. Edit All Aspirate Steps (Optional): If enabled in Settings, press the “Edit All” row to modify all aspirate steps simultaneously.

Running a Multi-Aspirate program involves multiple aspirations followed by a single dispense.

The Custom function allows you to create any type of program using unique step types like Aspirate with Turnaround, Dispense with Blowout, Purge, Instructions, Tip Change, Barcode, and Manual.

To edit a step in a Custom program, press on the step. The edit screen will appear. The main difference is that you first select the type of step you want to create. Once selected, the rest of the pop-up window options will display accordingly.

Unique Custom Step Types:

– Aspirate with Turnaround: For multiple dispenses from one aspiration. Aspirates beyond actual volume, then mechanically turnarounds. User must increase aspirate volume by a small discard amount (default 1% of nominal instrument volume, e.g., 3.0 µL for MPR300). This helps ensure final dispense accuracy.

– Instructions: Displays a headline and instructions to the user. Can include a countdown timer.

– Dispense All: Similar to standard dispense, but at the end, the unit goes to blowout, purging any remaining liquid.

– Tip Change: Inserts a Tip Load, Tip Eject, or full Eject/Load cycle. Options include specifying filter tips (instrument cannot verify), Manual Tip Load for partial cartridges.

– Mix: Define exact number of mixes. If starting at zero volume, default is to end with blowout (can be unchecked). If starting at non-zero volume, always stops where it started.

– Barcode: Capture/record barcode. Option for headline/instructions. Define barcode length. Add verification (Match Value, Match Variable, Set Variable, Check Digit).

– Manual: Incremental aspirate/dispense. Define initial increment, container, speed. During run, adjust increment and switch between aspirate/dispense.

Additional Step Options in Custom:

– Allow Return to This Step After Purge: If program is canceled/purged, allows returning to this step if logic permits.

– Run Automatically: Instrument performs step without pressing RUN button (not for Tip Change or Manual steps). CAUTION: For Dispense, Mix, Purge, ensure correct containers are in place.

Running a Custom program is similar to other programs, but with unique steps and flexible operation order, within instrument capabilities.

The Favorites section contains a scrollable list of all saved programs. Each program shows an icon (program type), name, description, and last modified date/time.

Sorting: Click the “Name” column title to sort. An arrow indicates sort direction; click again to reverse.

Searching: Use the search function at the bottom.

Loading a Program: Press a program to select it (row doubles in size, highlighted). Then press the “Load Program” tab at the bottom to open it for editing or running.

Deleting a Program: Swipe the program row to the left to reveal a red REMOVE button. Pressing it permanently deletes the program.

Import/Export: Access Import and Export functions directly from Favorites.

When editing or creating a program, press the SAVE tab at the bottom of the screen. A pop-up window will appear. Enter a name for the program and, if desired, a brief description. Then press SAVE. The program will be added to the list in Favorites.

NOTE: If you try to save a program with the same name as an existing saved program, you will be warned that the existing one will be over-written.

In Favorites, select a program, then press the “Versions” tab. This displays a list of all previously saved iterations of that program. From the Versions screen, you can:

– Select each version to view its details.

– Filter the list by a specific date range.

– Export some or all of the versions.

The “Load Tips” pop-up window will appear if there are no tips loaded upon startup (if that setting is enabled) or after a tip ejection.

To load tips:

1. Place the rack of new tips on top of the nest.

2. Bring the head down on top of the tips and press firmly. The instrument will automatically detect the presence of the tips and begin the loading process.

3. Continue holding the head down firmly for a few seconds until loading is complete.

NOTE: To skip tip loading and continue working without tips loaded, press the CANCEL button in the pop-up window. All programming and settings functions will be available. The “Load Tips” pop-up window will be displayed again once the RUN button is pressed.

The EJECT button is located on the Instrument Status Image on the left side of the screen, next to the tips image. Pressing this button will likely bring up a confirmation or initiate the tip ejection process.

Manual Tip Load is an option available in Custom programs under the Tip Change step. It is used for loading partial tip cartridges.

Refer to the “General Errors” section under Troubleshooting in the User Manual for guidance on resolving common issues.

Refer to the “Wireless Connection” section under Troubleshooting in the User Manual for steps to resolve connection issues between the MicroPro and the tablet.

To eject the current set of tips, press the EJECT button that is next to the image of the pipettor on the main screen. The pop-up window for ejecting tips will be displayed. First, place an empty rack on top of the nest. Then bring the head down such that the tips are just starting to enter the rack. Then press the RUN button on the instrument to eject the tips into the rack. Once ejected you will be prompted to load a new set of tips.

Caution: The tips will drop free. Place an appropriate container in the nest.

When enabled in Settings, the Manual Tip Load option allows you to load tips without relying on the automatic tip sensing and clamp mechanism. This allows for you to load a partial rack of tips in any configuration. When using Manual Tip Load, the pop-up window for manual tip loading will be displayed. The operation is identical to a normal tip load operation, except that after lowering the head onto the tips you will be required to push the Run button to initiate the clamp and sealing of the tips.

The “Load Tips” pop-up states: To load tips, place them in the nest and lower the head. Press firmly and hold; then Press Run to Seal. The instrument will clamp and seal onto the tips.

Filtered sterile and non-filtered sterile tips can be used with any MicroPro model. However, only the appropriate volume tips can be used with the matching model of the instrument.

CAUTION: In order to maintain proper performance, only use the tips approved for use with the particular MicroPro model that you are using.

The Pipetting Depth Recall (PDR) feature allows you to save the exact height offset relative to the bottom of a particular container. This provides a quick and reliable way of making sure that the tips are appropriately positioned away from the bottom surface. It also greatly increases efficiency and speed throughout all your operations.

After pressing CREATE NEW from within the container selection list or Settings, a pop-up window for finding the container bottom will be displayed. This window instructs: “Move the head of the pipettor until the tips touch the bottom of the container. Press Next to continue.”

First, place the container on top of the nest or below it (in the case of a reservoir). Then, bring the head of the base unit down such that the tips are touching the bottom of the container. Hold the head in this position and press NEXT to continue.

After pressing NEXT, a pop-up for adjusting tip offset will be displayed (showing 00.0 mm, Fine, Coarse buttons). This window instructs: “Press Save to confirm and save this container”. While continuing to hold the tips down, press the FINE and COARSE adjustment buttons until you have reached the desired distance between the tips and the bottom of the container. Then, press NEXT and the pop-up for entering the container name will be displayed.

After adjusting the tip offset and pressing NEXT, you will enter a name for the container and identify the nest location (Upper or Lower) on a screen with “Enter Container Name” and “Select Nest Location: Upper/Lower”. Then press SAVE to save this container. Afterwards you will be taken back to wherever you were when you started this process.

The nest platform can be easily converted to allow aspirating from or dispensing into 384-well microplates. Each side of the nest is labeled according to the size of microplate it is designed for. To change sides, simply loosen the nest mounting thumb nut, flip the nest platform over and re-secure with the thumb nut.

When using the nest for 384-well microplates, place the microplate onto the nest and push it into each corner. Each corner will align the tips with a specific quadrant of the 384-well microplate. You can aspirate or dispense normally.

Settings enable you to adjust a number of different options, view calibration status, setup liquid classes or containers, import/export files, view the data logs and access the advanced settings menu. The complete list of categories available is on the left side of the screen. Once selected, the detailed options appear on the right side. The BACK button on the bottom left will return you back to the main screen. Whenever a setting has been modified the SAVE button will become available on the bottom right. Once you are satisfied with the changes, simply press the SAVE button to apply those changes.

NOTE: If you attempt to leave a section after changes have been made without saving them, a warning pop-up will appear to inform you that the changes will be lost unless they are saved.

The first Settings category is Pipetting, where you can enable the “Edit All” function, show the RUN button and adjust the Purge Speed.

Checking the box to allow “Edit All” enables this function within the Variable Dispense and Multi-Aspirate programs.

Checking the box next to show RUN Button will display the RUN button on the main screen. It will be located at the top of the instrument status image. This feature allows you to run each step of the program remotely from the tablet. For example, this can be quite useful if the instrument is under a hood during operation.

The Show Elapsed Time option displays the total time that has elapsed while running a program. The clock starts as soon as you run the first step and will stop upon completion of the last step. This will allow you to track how long it takes to run a particular program or protocol.

The Purge Speed setting defines the speed at which all Purge operations are conducted (options shown are 1-10). Whenever there is a Purge step in any program, it will be done at the speed set here. There is also a Homing button that, when pressed, allows you to home the instrument.

The screen also shows checkboxes for “Allow Edit All”, “Show Run Button (When connected to a physical instrument)”, and “Show Elapsed Time”.

This section displays information about the instrument and the MicroPro Controller app. Specifically, it shows the current Instrument Name, Model Number, Serial Number, Firmware Version, App Software Version, Date and Time the current version of the app was installed, the Language and the total number of programs saved.

The two things you can do in this screen are change instruments and modify the instrument name. To edit the name of the current instrument, press the button that the name is in. This allows you to edit and rename the instrument to anything you wish. Pressing on the “Change Instrument” button allows you to connect to any other available instrument.

NOTE: The Instrument Name is what will appear in the list of available instruments in the Connection screen. This is also the name that will be displayed on the main screen after you are connected.

Here you can turn on and off the deck lighting, as well as control the brightness (options shown are off, 1, 2, 3, 4, 5). Finally, there are two sound effect options.

The Play Chime After Every Step option will trigger a simple ding sound after the completion of every step in any program that is being run.

The Play Chime When Instruction Timer Ends will only play the ding sound after the timer on an instruction step ends. This can be helpful if you have stepped away from the instrument while it was running, as it will notify you upon the completion of each step.

Checkboxes are shown for “Play Chime After Every Step” and “Play Chime When Instruction Timer Ends”.

This section displays the current calibration status, type, current values, date last modified and the name entered when it was last modified. You can also view the Calibration Log, which will have the complete history of any and all changes made to the instrument calibration. The status of the maintenance reminder is also shown on this screen (e.g., Reminder: Duration, Needed After, Last Reset: Disabled).

Example fields shown: Calibration Type, Current Values, Last Modified, Modified By, Factory Calibration Date. There is a “View Calibration Log” button.

Here you will create and maintain the calibration settings for all the special liquids that you use. The list contains the name of each liquid class, their settings and creation date. Liquid classes can be used during any sequence of pipetting operations in any program. Within each Aspirate step edit pop-up or screen you can select one of these liquid classes to use. Each following step in the sequence will continue to use the active liquid class specified unless changed. A “Versions” button is also available.

The screen shows a table with columns: Name, Calibration Values, Date. Example entries: “< Create New >”, “Liquid #1”, “Liquid #2”, “Liquid #3”.

To create a new liquid class, press on the top row labeled Create New in the Liquid Classes settings. The pop-up window for creating a new liquid class will be displayed. Here you can enter in the name of the special liquid and the desired calibration values (Selected High Calibration Point, Selected Low Calibration Point), along with the subsequent actual values (Actual High Value, Actual Low Value). Specify the values using either a One Point or Two Point calibration (checkbox for “One Point Only”). You can also specify the pipetting speeds (1-10 check boxes for Allowed Aspirate Speeds and Allowed Dispense Speeds) that are allowed to be used with each liquid class defined.

To modify or remove a liquid class that has already been created, swipe that row to the left in the Liquid Classes list to reveal the Modify and Remove buttons.

Once you select a liquid class, you can also press the Versions button which will display all the previously saved iterations of that liquid class. Here, you can also view and export detailed specifications of each version.

When actively using a liquid class other than the system default, a blue icon with the name of the selected liquid class is shown on the main screen next to the instrument image.

Here a complete list of all saved containers is organized by name and displays the offset distance and date it was created or last modified. It also shows the nest position (Upper or Lower) used when the container was created. A “Versions” button is also available.

The screen shows a table with columns: Name, Offset, Date. Example entries: “< Create New >”, “Plate #1”, “Plate #2”, “Plate #3”.

You can create new containers by simply pressing < Create New > on the top row in the Containers list. To modify or remove existing containers, swipe the desired row to the left to reveal the corresponding buttons.

This section offers four options: Import, Export Custom, Export All and Manage Files.

Selecting Import brings you to a new screen where you can import any combination of programs, containers and liquid classes from the JSON files on your iPad. The first screen lists those files (with Name and Date) and allows you to select any number of them to import. After selecting the desired file(s) and pressing the NEXT button, a new screen is shown. This screen has one tab each for programs, containers and liquid classes. After making a selection from the Programs Tab, press the Include Containers and Liquid Classes button to automatically select all containers and liquid classes used by the selected program(s). This helps ensure that you import all the items necessary to run the desired programs. Once finished, press the Import button to bring all the items into the app.

Choosing Export – Custom will take you to a screen where you can export any combination of programs, containers, liquids and logs. The screen for containers allows selecting plates with Name, Offset, and Date. The log screen allows you to select the log and the start/end date that you wish to export. If you do not specify a date range for the log, the entire log will be included.

After making your selection and pressing the Export button, a pop-up window (Export Summary) displays a summary of all items that are being exported (Programs, Containers, Special Liquids, Logs with counts). You can also modify the name for the JSON file and decide whether you would simply like to save it to Files on the tablet or send it to another device via Airdrop. If you choose Airdrop, the standard options are made available. Using Airdrop is an extremely simple way of sharing programs from one tablet to another.

If you choose Export All, you will be presented with a screen where you have the ability to select the complete set of files for each category. The categories include: Programs (All Versions), Containers (All Versions), Liquid Classes (All Versions), Pipetting Activity Log, Connection Log, Settings Log, Calibration Log, Error Log, System Log.

After making your selection and pressing the Export button, a similar export summary pop-up window will give you the option to modify the name of the JSON file and, again, choose whether to save it to Files on the tablet or use Airdrop. This is the fast and simple way of creating a complete backup of the entire app. If you ever lose the tablet, use this file to restore the replacement tablet to the state the lost tablet was in when the Export operation was executed.

NOTE: The logs cannot be imported, but the information is still available within the JSON file.

This section shows the data logs for the instrument. View each log by pressing on the name and specifying a date range (Start Date YYYY/MM/DD, End Date YYYY/MM/DD). If no date range is specified, the entire log is shown. The log types listed are: Pipetting Activity Log, Connection Log, Settings Log, Calibration Log, Error Log, System Log, Login/Logout Log.

The Pipetting Activity Log displays every program and tip eject/load operation that has been executed using this particular tablet. Each entry contains the type, name, version number, description, date and timestamp of when it was executed. Selecting each entry shows the details of the program or operation. From here you can also modify the date range of entries being displayed as well as sort them by date and time, name, version and type.

The Connection Log is a record of each connection or attempted connection that this tablet made to an instrument. Each entry contains the serial number of the instrument as well as the date and time of the event. Select any entry to view additional details.

The Settings Log is a record of every change made to any of the app settings, including the name of the setting, the value that was changed and the date/time of the modification. Selecting a particular entry will bring up the details on its own screen.

The Calibration Log is a record of each change made to the calibration of an instrument using this tablet. This includes factory and user calibrations. Each entry will identify the instrument serial number, type of calibration, calibration values, the name entered when the changes were made and the date/time of the change. Selecting an entry provides a detailed view.

The Error Log is a record of any errors that a connected instrument or the app detected. Each entry will include the serial number of the instrument, the name or description of the error, and the date/time that it was detected. Selecting a particular entry brings up the details on its own screen.

The System Log is a record of all the actions performed by the system. Each entry contains the serial number of the connected instrument, name of the action performed and the date/time it was executed. Selecting an entry provides a detailed view.

The Login/Logout Log is a record of each time the app was opened or started while the Require User Name setting was enabled. If that setting is enabled, every user will be required to make an entry under their username in order to start the app. These entries are recorded here and include the username entered and the date/time they started the app. Selecting a particular entry brings up the details on its own screen.

This section provides a new submenu of advanced options, including calibration and security. A complete list of sections is in the left column, just like the standard settings screen. Pressing the BACK button returns you to the standard Settings screen. Navigating and saving changes here works exactly like it does on the regular settings screen.

Advanced Pipetting provides a number of options that will affect the normal operation of the MicroPro Controller app. These include:

Allow Extreme Low Volumes: This option reduces the minimum pipetting volume from the standard 5 µL down to 2 µL for the MPR300 and from 1 µL down to 0.5 µL for the MPR20 and from 50 µL down to 20 µL for the MPR1250.

Enable Manual Tip Load.

Tip Load on Connect: This option immediately shows the tip load pop-up window upon connection to the instrument.

Use Start Screen.

Require Username.

Allow Run Step Automatically: Checking this option allows all users to enable this functionality on any custom program step where it is allowed.

Default Blowout Volume: You can modify the default blowout volume. The screen shows Current Default Blowout Volume and Factory Default Blowout Volume, with an editable field for Default Blowout Volume.

CAUTION: Allowing extreme low volumes will reduce pipetting accuracy at those volumes. It is strongly recommended that you verify performance prior to pipetting below the standard allowable volume range of your instrument.

The Use Start Screen setting shows a new screen that displays a large start button when initially connecting to an instrument. There is also an Exit button in the upper left hand corner of the main pipetting screen that takes you back to this start screen.

You also have the option to Require User Name. This provides a text box where all users must make some entry (e.g., “Jane Smith”) before progressing to the main pipetting screen. This entry is recorded in the logs for any operation performed by this user during this session.

NOTE: Username is not verified in any way, so the app will accept any entry of valid characters.

The Advanced Pipetting screen also provides the option to modify the default blowout volume. This will universally affect all programs where a blowout is performed and the actual value depends on the MicroPro model being used.

By default the instrument operates under Factory Calibration. This is the normal operating state that has been calibrated by the factory to ensure the accuracy and repeatability of the instrument across the normal operating volume range. These settings cannot be altered, however Rainin recommends that users follow Good Laboratory Practices (GLP) and perform maintenance checks at regular intervals to ensure proper operation. If desired, other types of calibration are available. You can select from either a Custom One Point or Two Point calibration. This will adjust the settings of the instrument on top of the factory calibration. Options shown are checkboxes for Factory Calibration, Custom One Point, Custom Two Point.

After selecting a Custom Two Point calibration, the screen for custom two point calibration is displayed. Here you can enter the desired calibration values (Selected High Calibration Point, Selected Low Calibration Point) along with the subsequent actual values (Actual High Value, Actual Low Value). You also enter the “Modified By” name (e.g., Jane Doe). This will adjust the instrument’s calibration curves accordingly. Current Values, Last Modified date/time, and Last Modified By are also displayed. Buttons for “Calibrate Upper Nest” and “Calibrate Lower Nest” are present.

A Custom One Point calibration is functionally the same as a Two Point calibration, but with only a single adjustment point.

There is an option to re-calibrate the upper and lower nest positions. The nest positions are calibrated at the factory, however you may need to re-calibrate the nest position if using a Dual Nest Accessory. The process is simple: to calibrate the lower nest, simply press on the Calibrate Lower Nest button in the Calibration settings to display a pop-up window.

The pop-up instructs: “Find Lower Nest Position. Move the head down until the tips pass through the upper nest and touch the table. Press Next to continue.”

Follow the instructions and lower the head until the tips pass through the upper nest and rest on the table or lower nest position. Continue to hold the head in this position and press the NEXT button. The instrument will automatically detect and re-calibrate the lower nest position. Once this is complete, a new pop-up window will tell you that the nest calibration is complete.

To calibrate the upper nest, simply press on the Calibrate Upper Nest button in the Calibration settings to display a pop-up window.

The first pop-up instructs: “Place Sticky Note. Place a standard sticky note or a small piece of paper on top of the upper nest, making sure it is completely within the recess. Press Next to continue.”

Follow the instructions and place a single sticky note or small piece of paper on the upper nest. Then, bring the head down until the tips are resting on the paper as shown in the second pop-up window. The second pop-up instructs: “Find Upper Nest Position. Move the head down until the tips gently touch the sticky note / paper. Press Next to continue.” Do not press so hard as to deform the paper.

Continue to hold the head in this position and press the NEXT button. The instrument will automatically detect and recalibrate the upper nest position. Once finished, a similar completion message will be displayed.

Here you can turn on/off the Maintenance Reminder (checkbox) and set its duration in days (e.g., 90 days). You can also reset the reminder (Reset button), see when it expires (Reminder Expires: YYYY/MM/DD), and see when it was last reset (Last Reset: YYYY/MM/DD). The screen also shows “Maintenance Due XX days”. This is a helpful tool that can ensure the instrument is checked at regular intervals.

When the reminder elapses, a red exclamation point symbol is shown next to the image of the pipettor on the main screen. Pressing on this symbol will tell you that it is time for maintenance.

This section provides access to a number of tools for analyzing and diagnosing potential issues with the instrument. Specifically, you can put the app into a “Debug Mode” (checkbox) that will display some additional information that might be helpful when investigating a particular issue. You can also view the Pipetting Errors log directly from here (button “Pipetting Errors”).

NOTE: Issues with the app and instrument are rare. Even so, Rainin Technical Support can assist you in resolving any issues as quickly as possible.

You can set two levels of password protection for the MicroPro Controller app. Check boxes allow you to turn each level of protection on/off independently.

Advanced Menu password protection: When turned on (checkbox “Advanced Menu” under “Set Password Protection:”), users will only be able to access the Advanced Settings menu if they enter a password. This helps protect some of the more critical settings, such as Calibration, from inadvertent changes. A field for “Password” is provided.

App Lockdown protection: When App Lockdown protection is enabled (checkbox “App Lockdown”), the user will only be able to run pre-existing programs. They will not be able to edit existing programs or create new ones. A field for “Password” is provided.

When activating this feature, ensure the box is checked, then enter the desired password and press SAVE. Once you exit this section you will be required to enter the password before you can return and modify the security settings.

Write down your password in a safe place. However, when this security feature is enabled, a Password Recovery button will also be displayed. If you forget your password, press this button and you will be provided with a special code. Write this code down and contact Rainin Technical Support at tech.support@rainin.com. They will use this code to recover your password.

This area is for service personnel only and is password protected. If you need assistance with your instrument, contact Rainin technical support at tech.support@rainin.com.

This section provides access to potential new MicroPro Controller app features being evaluated. These features are still in the beta test phase and therefore their performance and functionality cannot be guaranteed. For more information about these features or to provide your feedback, please contact your local sales representative.

Here, you can change the native language for the MicroPro Controller app. The language options include French, German, Italian, Spanish, Japanese, Korean, Chinese (Simplified) and Chinese (Traditional). When an alternate language to English is selected, the complete user manual found in the Help section will also be updated to match that language.

NOTE: The language selection within the app is separate from any language or keyboard preferences found in the main settings of the tablet itself.

The following table outlines common general errors, their possible causes, and solutions:

The following table outlines common wireless connection errors, their possible causes, and solutions:

We recommend that the instrument be cleaned at least once a month. All outer surfaces of the instrument can be cleaned using a clean cloth and a mild detergent. For easy cleaning of the base, remove the nest to easily clean both sides. The nest is autoclavable if necessary.

WARNING: The instrument must be disconnected from electrical power prior to cleaning in order to avoid the risk of electric shock and damage to the instrument.

CAUTION: Avoid spilling liquid onto the pipetting head or into the instrument tower. This could lead to instrument damage and/or improper functionality. Do not use any aggressive solvents or chemicals on the instrument. This could result in damage to the instrument and/or improper functionality.

Parts that may have been contaminated with potentially hazardous substances should be cleaned with a damp cloth and 10% bleach solution or any other appropriate disinfectant. Decontamination is the sole responsibility of the owner and/or operator of the instrument. For more information, or if you have any questions regarding decontamination contact, Rainin Technical Support at tech.support@rainin.com.

Prior to sending a unit in for service, or having the unit serviced in the field, it must be decontaminated if it has come into contact with radioactive or hazardous substances.

METTLER TOLEDO and Rainin provide service and calibration for the MicroPro. We recommend preventive maintenance and calibration be completed on your instrument at least once a year or according to your internal SOP. ISO 17025 certificates are available and can be requested from your local sales representative. Any service must be performed by a factory-trained technician with original parts to maintain instrument reliability and performance.

WARNING: Do not open the instrument enclosure. There are no user-serviceable parts inside.

Save all the packaging materials that came with your instrument. Follow the packing instructions when packing up your instrument for shipment or transport. The first step involves checking box contents, and the second is to unplug the instrument.

Details regarding the standard test conditions are available upon request. Please contact Rainin in the U.S. or your local METTLER TOLEDO office. In the U.S. dial +1 800 4 RAININ (800 472-4646) or email techsupport@rainin.com.

To order tips, accessories or additional instruments, please contact your local Rainin sales representative in the U.S. or your local METTLER TOLEDO office. In the U.S., call +1 800 4 RAININ (800 472-4646).

The following MPR tips are available in racks (96 tips/rack). LR: Low retention S: Sterilized F: Filtered

The following MPR tips are available in strips (8 tips/strip; 12 strips/rack). LR: Low retention S: Sterilized F: Filtered

The following Two-Tray Dual Nest accessories are available: