EPPENDORF 5417 R (01) PDF Document

When removing the centrifuge from the packaging, take hold of it at the sides, support it from underneath and lift it onto the lab bench.

To install the device properly, follow these steps:

Remove the transport safety devices according to the instructions on the pack insert and save these for possible future transport of the centrifuge. Only tilt the device slightly; do not place it on its side.

To avoid damage to the compressor caused by incorrect transportation, wait four hours after installation before switching on the device.

Place the centrifuge onto a stable, vibration-free, horizontal surface. Make sure that the centrifuge is not exposed to direct sunlight.

To ensure sufficient ventilation, there should be clearance of 15 cm on both sides of the centrifuge to the wall or other devices. According to recommendations of the EN 61010-2-020 standard, a safety distance of 30 cm should be observed around the centrifuge during operation.

Connect the centrifuge to the main supply and press the main switch located on the right-hand side of the device, directly next to the main connection.

The centrifuge lid can now be opened by pressing Open. Insert the rotor before starting and tighten.

The rotor and the rotor cover must always be securely fastened. Do not begin centrifugation before the rotor has been securely fastened.

The rotor must be loaded symmetrically. Opposing tubes should be of the same type and should be filled equally.

Do not use buckets and rotors which show clear signs of corrosion or mechanical defects. Check accessories at regular intervals.

Do not move or knock against the centrifuge during operation.

The device must not be operated in a hazardous or flammable environment and must not be used to centrifuge explosive or highly reactive substances.

A liquid density of 1.2 g/ccm must not be exceeded at the maximum rotational speed.

Close the test tube lids before centrifuging. Open lids can be ripped off during centrifuging and damage the centrifuge.

Rotors are high-grade components subject to extreme mechanical strain. Ensure that the rotor is protected from mechanical damage. Even slight scratches and cracks can cause severe inner damage to the rotor materials.

Clean the motor axle and the rotor bores with a cloth before attaching the rotor.

When fastening the rotor onto the motor axle, ensure that the temperature of the rotor and the motor axle is between 10 °C and 30 °C.

Mount the rotor onto the motor axle and tighten the rotor nut by turning it clockwise using the hexagonal key supplied.

To dismount the rotor, turn the rotor nut counterclockwise using the hexagonal key.

The aerosol-tight rotor can also be removed from the centrifuge when the lid is closed; this is done by inserting the hexagonal key into the opening in the lid screw.

Do not centrifuge using rotors and buckets with visible corrosion or mechanical defects.

Switch on the mains supply. The nominal values of the last run appear in the display.

Load the rotor symmetrically and close the centrifuge lid. The green lamp in the Open key lights up. If “LID” lights up in the display field, close the lid again.

To modify the values for the rotational speed / rcf (relative centrifugal force), time and temperature: The new nominal value appears in the display.

When both arrow keys are pressed simultaneously, the centrifuge switches from “rpm” (rotational speed) to “rcf”. The rcf symbol appears in the display.

To set the run time (minutes / seconds), use the time selection keys. The remaining centrifugation time is displayed in minutes, with the last minute being counted down in seconds.

Press Start/Stop to start the run. The key flashes as long as the rotor is spinning.

During the run, the rotational speed of the rotor or the appropriate rcf value, the sample temperature and the remaining spin time in minutes are displayed. During the run, all parameters can be modified.

Press Start/Stop to end the run. The rotor is braked and brought to a standstill. During the braking process, the time display flashes and the elapsed spin time is displayed.

Press Open to open the lid when the control lamp in the key is lit up. The symbol shows that the rotor is not moving.

The Short key should be held down for the intended duration of the run. The time which has elapsed is displayed in seconds. A run which has already been interrupted can be restarted by pressing the key again, provided that the centrifuge has not come to a standstill.

Runs with a duration of 10, 20 or 30 seconds may be set using the time selection.

If the Short key is pressed when the lid is open, one of the following six status displays appears: “10 t” or “1-10 t” / “14 t” or “1-14 t”/”16 t” or “1-16 t”.

If the key is held down for longer than three seconds, the device switches to the other status and displays this for two seconds. The way which was last set is retained and appears in the display.

The meaning of the status displays is as follows:

“10 t”: The swing-bucket rotor runs to its maximum speed of 10,500 rpm.

“14 t”: The 30-position fixed-angle rotor runs to its maximum speed of 14,000 rpm.

“16 t”: The 24-position fixed-angle rotor runs to its maximum speed of 16,400 rpm.

“1-10 t”: The speed can be selected with the cursor keys (swing-bucket rotor).

“1-14 t”: The speed can be selected with the cursor keys (30-position fixed-angle rotor).

“1-16 t”: The speed can be selected with the cursor keys (24-position fixed-angle rotor).

When the Soft key is pressed before or during acceleration, the acceleration and braking phase is extended to approximately 60 seconds. This is indicated by the control lamp in the key.

The Soft mode cannot be switched off after acceleration has finished.

The nominal temperature can be set from – 9 °C to + 40 °C. It can also be modified during the run.

Once the nominal temperature set has been reached, a deviation greater than ± 3 °C is indicated by a flashing temperature display. If the temperature deviates by more than 5 °C, the centrifuge switches off with a periodic warning signal.

Fast Cool: To start a temperature-control run at a rotor-specific speed, press Fast Cool. Refrigeration enables the new, preset temperature in the rotor to be reached rapidly. The run ends automatically or by pressing the Stop key. A periodic warning signal is emitted. Cooling from RT to 4 °C lasts approximately 20 minutes.

Standby refrigeration: When the lid is closed, the rotor chamber is refrigerated to the preselected nominal temperature before or after a run as long as this value is below ambient temperature. The rotor does not spin during this procedure and the temperature is adjusted more slowly. The nominal temperature appears in the display.

To protect the device, refrigeration switches off automatically if the centrifuge is not used for longer than six hours or if the lid is not opened. “Standby off” and the actual temperature in the chamber appears in the display.

Please empty and clean the tray for condensation water (located below the device) at regular intervals. This tray can be removed by pulling it out towards the front.

If the magnetic lid latch cannot be activated because of a power failure, the emergency lid release can be activated manually.

1. Turn off the main switch. Wait until the rotor has come to a standstill.

2. Open the lid carefully, as the rotor may continue spinning for some time. Disengage the lid latch by pressing the lid down slightly.

3. A plastic knob is situated at the left front edge of the base plate (labeled E). After this knob has been pulled out and then downwards, the emergency lid release can be activated.

4. The lid latch is disengaged by pressing down the lid lightly. The knob must be then reinserted.

The outside of the centrifuge and the rotor chamber should be cleaned regularly with neutral detergent. This is for hygiene purposes as well as to prevent contamination caused by residual contamination.

Open the lid of the centrifuge and disconnect the main plug. Unscrew the rotor with the hexagonal key provided and clean separately.

Only neutral agents may be used for cleaning and disinfection (e.g. diluted Extran neutral, RBS neutral or 70% isopropanol/water mixture or an alcohol-based disinfectant).

The rotor chamber should only be cleaned with a moist cloth.

After cleaning with detergent, the rubber seals in the rotor chamber should be rinsed well with water and lubricated with glycerine.

For refrigerated centrifuges, remove any condensation water in the rotor chamber using a soft, absorbent cloth. Switch off the centrifuge after use, leave the lid open and empty the tray for condensation water, located below the device.

In the event of glass breakage, carefully remove all splinters and all ground glass from the rotor, the buckets, the adapters and the rotor chamber.

You may need to replace the rubber plates and adapters to prevent further damage.

Fine splinters of glass may otherwise scratch the surface of the rotors and buckets, reducing their resistance to chemicals. The air eddies in the rotor chamber produce a very fine black cloud of abraded metal which, in addition to causing damage to the rotor chamber, rotor, buckets and adapters, also results in samples becoming contaminated.

Check the rotor bores regularly for residues and damage.

If the above solutions are unsuccessful, please contact SERVICE.

Power requirement: Centrifuge 400 W; Compressor 300 W

Max. rotational speed: 16,400 rpm

Max. centrifugal force: 25,000 rcf

Max. load: 30 x 2.0 ml Safe-Lock Microcentrifuge Tubes

Max. density of material to be centrifuged: 1.2 g/ml

Range of control for refrigeration: – 9 °C to + 40 °C

Refrigeration time for “Fast Cool”: from 20 °C to 4 °C: approx. 20 min.

Permitted ambient temperature during operation: 10 °C–35 °C

Permitted maximum relative air humidity: 75 %

Max. kinetic energy: 6,100 Nm

Dimensions (H x W x D): 250 x 310 x 597 mm

Weight: 34.8 kg