WOODLEY INSIGHT V5R (01) PDF Document

The main function buttons on the WOODLEY INSIGHT V5R display and their purposes are:

Main Menu/settings: Accesses the main menu and settings of the analyser.

Sample Analysis: Used to analyse a sample.

Review: Allows you to review previous results.

QC: Used to run Quality Control (QC), enter QC values, and review QC history.

Reagent Management: Allows you to change reagents.

Other areas of the screen include the Patient Information Area, Analysis Results Area, and Information of the next sample.

To run a sample on the WOODLEY INSIGHT V5R, follow these steps:

1. Use an EDTA K3 anticoagulated blood collection tube.

2. Fill 2/3’s of the tube with blood.

3. Ensure a background count and auto clean has been performed before analysis.

4. Use the touch screen to select the ‘Sample Analysis’ screen.

5. Select ‘Next Sample’.

6. Enter Patient details including species. If testing Reticulocytes, ensure to select the sample mode as CBC-DIFF-RETIC, then press ‘OK’ to save.

7. Thoroughly mix the blood on a roller mixer or by slowly inverting the tube 10 times.

8. REMOVE THE SAMPLE CAP and place the sample under the probe.

9. Push the sampling trigger.

10. Remove the sample when the analyser ‘beeps’.

11. Results will automatically display and print out once the analysis is complete.

When you receive a new haematology control vial with a different lot number and expiry date, you must enter the new normal ranges into the WOODLEY INSIGHT V5R control file. Follow these steps:

1. Select ‘QUALITY CONTROL’.

2. Select ‘QC settings’.

3. Select ‘New’.

4. Using the information supplied on the new QC assay sheet, enter the following information:

– The new QC batch lot number (e.g., DH2408)

– Expiry date (dd/mm/yyyy)

– Target values and limits

5. Press the ‘Save’ key to save the QC information.

6. Go into ‘QC Analysis’ and ensure the correct file number for the current lot number of QC is selected.

7. Run the QC.

NOTE: Keep QC vials refrigerated and allow at least 15 minutes to reach room temperature (18-30°C) before use. After sampling, wipe the threads of both the vial and cap with a lint-free tissue. Replace the cap and return to the refrigerator for maximum open vial stability, which is up to 14-28 days if stored at 2-8°C.

Running a Quality Control (QC) on your WOODLEY INSIGHT V5R is recommended at the start of each working day for the following reasons:

– To assess the quality and performance of the practice laboratory.

– To ensure that results generated by the InSight V5R are correct.

– QC monitors staff, equipment, reagents, and result reporting.

Follow these steps to run a QC on the WOODLEY INSIGHT V5R:

1. Navigate to the QC test screen by selecting ‘QUALITY CONTROL’.

2. Select ‘QC Analysis’.

3. Ensure the correct file number for the current lot number of QC is selected.

4. Ensure that the QC has been stored correctly, is in date, and check that the vial lot number and expiry date correspond to those on the screen. If it’s a new batch, enter the new QC reference ranges.

5. Thoroughly mix the blood by slowly inverting the tube 10 times. DO NOT use a rotary mixer. Make sure the sample is warmed to room temperature.

6. REMOVE THE SAMPLE CAP and place the QC sample under the probe.

7. Press the back plate to start the analysis.

8. Results will automatically display once the analysis is complete.

9. Check that the QC is within the ranges.

10. Press ‘QC Graph’ to open the Levey-Jennings graph screen.

11. Sign the QC log sheet under the appropriate date and return the QC to the fridge.

NOTE: Keep QC vials refrigerated and allow at least 15 minutes to reach room temperature (18-30°C) before use. After sampling, wipe the threads of both vial and cap with a lint-free tissue. Replace cap and return to the refrigerator for maximum open vial stability which is up to 14-28 days if stored at 2-8°C.

In the event of a QC failure on your WOODLEY INSIGHT V5R, follow these steps:

1. Reprocess the sample.

2. If it’s still out of limits, repeat with a fresh QC.

3. If the failure persists, contact your Woodley Sales Representative or Woodley Equipment Company Technical Support for assistance.

When the ‘Insufficient reagent’ error appears on your WOODLEY INSIGHT V5R, follow these steps:

1. Remove the cap from the new reagent and connect the reagent tubes from the old reagent bottle to the new bottle.

2. Select ‘Remove error’ on the screen.

3. Scan the RFID card.

4. The analyser will automatically prime the new reagents.

To change reagents using the Reagent Management screen on your WOODLEY INSIGHT V5R, follow this procedure:

1. Remove the cap from the new reagent and connect the reagent tubes from the old reagent bottle to the new bottle.

2. Select the ‘Reagent Management’ tab at the top of the screen.

3. Select ‘Setup’.

4. When indicated, place the RFID card for each reagent bottle on the RFID reader on the front of the analyser. (NOTE: the FDF-R AND DIL-F RF cards are smaller and attached to the side of the bottle).

5. The analyser will indicate that the reagent information has been set successfully.

6. Select ‘Replace’. The analyser will prime the reagent(s) and the consumption counters will be reset.

7. After the reagent change, run a Blank measurement to ensure no bubbles remain in the system.

**Instrument waste can be safely disposed from the waste container into a sink if accompanied by running water.

Standby: The WOODLEY INSIGHT V5R is automatically programmed to go into standby each day and does not need to be switched off manually.

Wakeup/Start up cycle: Touch the screen to exit the standby mode and push the back plate to start the startup cycle.

Shutdown: At the end of the week, the WOODLEY INSIGHT V5R needs to be shut down. Select the ‘Off’ icon on the main screen. Click ‘Yes’ to perform the shutdown. The analyser will prompt you to place cleanser under the probe. When prompted, the analyser can be switched off at the back.

Startup Cycle: If the WOODLEY INSIGHT V5R is powered off, first switch the power on at the back of the analyser. Login as user: admin password: admin. The analyser will automatically complete the startup cycle and a background count. Check that the background measurement is acceptable, then the system is ready to process a QC and then blood samples.

The WOODLEY INSIGHT V5R uses the following flags to indicate potential issues or abnormalities in haematology results:

Proper sample handling is critical for accurate results from the WOODLEY INSIGHT V5R. Follow these guidelines:

Venipuncture: Perform a quick, non-traumatic venipuncture. Use a 22-gauge needle or larger to minimize cell damage (haemolysis) and clot formation. Avoid repositioning the needle and/or excessive suction. Fractious cats may have platelet aggregates; consult a blood film if in doubt.

Sample transfer: Immediately transfer the blood sample into an EDTA tube. Remove the needle and slowly express the sample down the side of the tube to prevent haemolysis and clots. DO NOT use heparin tubes.

Sample volume: Fill the tube adequately (at least ½ full). Under-filled tubes have an incorrect EDTA-to-blood ratio, which can cause cell shrinkage and lead to falsely decreased MCV and HCT.

Sample mixing: Thoroughly mix the sample either on a mixer or with 8-10 inversions by hand up until placing it in the analyser. Check the tube for clots before sampling. DO NOT test a clotted sample; take a repeat sample.

Time delay: Process blood samples as soon as possible after collection. Platelets have a short half-life and can clump over time. Platelet counts performed after 4-6 hours should be considered suspect. Prolonged storage, especially if not refrigerated, can cause RBC swelling and a falsely elevated MCV. If there is a delay, store the sample at 2-8°C. Excessive temperatures can lead to cell autolysis.

A Cleanser Soak should be performed daily or when background results exceed the range, you have bad differential results, or if a clot remains in the system after other maintenance procedures. The WOODLEY INSIGHT V5R analyser will prompt you when the soak is due.

To perform the soak:

1. Select ‘Service’ from the menu.

2. Select ‘Maintenance’ and then ‘Cleanser Soak’.

3. A prompt will appear asking ‘Perform Cleanser Soak?’. Select ‘Yes’.

4. The analyser will instruct you to “Present Cleanser to the sample probe, then press the aspirate key…”.

5. Present the Cleanser under the sample probe and press ‘Aspirate’.

6. Remove the Cleanser after a beep.

The Cleanser Soak will take approximately 20 minutes to complete.

The Red Blood Cell (RBC) histogram on the WOODLEY INSIGHT V5R provides a graphic representation of cell size (Mean Corpuscular Volume, MCV) versus cell number. The horizontal axis represents the size (Smallest to Largest in fL), and the vertical axis represents the number of cells. The position and shape of the peak can indicate the characteristics of the RBC population. It is possible to observe microcytic (small red cells) and macrocytic (large red cells) populations from the size and position of the peaks. You should be familiar with the appearance of a normal histogram to make a useful assessment of abnormal samples.

On the WOODLEY INSIGHT V5R, as each White Blood Cell (WBC) passes through the flow cell, a laser beam shines through it, and the light is scattered depending on the contents of the cell. The intensity of this scattered light reflects the blood cell size and intracellular density.

The scattergram plots these signals:

– The low-angle scattered light signal (LS) shows cell size.

– The middle-angle (MS) and high-angle scattered light signals show intracellular information (nucleus and cytoplasm information).

An optical detector converts this scattered signal into electrical pulses, creating a scattergram where different cell populations (e.g., Lymphocytes, Monocytes, Neutrophils, Eosinophils) form distinct clusters.

Platelet (PLT) histograms on the WOODLEY INSIGHT V5R are used to determine the volume distribution of platelet populations. The horizontal axis represents size (Mean Platelet Volume, MPV), and the vertical axis represents the number of platelets.

These histograms are useful for assessing thrombocytopenia. For example, increased consumption of platelets often leads to the production of large platelets, which are depicted on the right side of the histogram, increasing the Platelet Distribution Width (PDW).

Platelet histograms can also help assess if a low platelet count (thrombocytopenia) is genuine or due to a sample quality issue, such as aggregates. If platelets are aggregated, an atypical picture with an uneven distribution is seen on the histogram.

The recommended maintenance schedule for the WOODLEY INSIGHT V5R is as follows:

Daily Maintenance (Customer Maintenance)

– Startup/wakeup cycle (Refer to section 6)

– Shut down/sleep mode (Refer to section 6)

– Program for each evening

– Perform manual shutdown at the weekend then switch off the InSight V5R

– Visually check reagent levels

– Clean cover

– Blank Measurement

– Cleanser Soak

Weekly Maintenance (Customer Maintenance)

– Clean Probe

Monthly Maintenance (Customer Maintenance)

– Clean Fluidics

Yearly Maintenance (Woodley Service Maintenance)

– Full Service

Please contact Woodley Equipment Company Ltd for information about a fully comprehensive service contract.