AGILENT 1290 INFINITY II MULTISAMPLER G7167B (01) PDF MANUAL

CAUTION

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

Unmatched flexibility – You choose how you want to introduce samples for injection, whether you prefer vials, microtiter plates, or any combination of formats. Sample drawers are available in three heights, and you can mix shallow drawers with deeper ones to accommodate different sample sizes.

High capacity – Using shallow well-plate drawers, the 1290 Infinity II Multisampler takes a maximum load of 16 microtiter plates and up to 6144 samples-the most of any single system.

Seamless automation – Internal robotics move microtiter plates and other sample containers from the sample hotel to the central workspace for sample processing steps and injections.

Dual-needle injection – By running samples alternately through one or the other injection path, you can reduce cycle times to mere seconds, virtually eliminating conventional wait times-whether for large volume loadings or flushing procedures.

Scalable injection volumes – The Agilent unique dual-needle setup also enhances flexibility by providing two differently optimized injectors in a single instrument. You can, for example, optimize one path for large volume injections and the other for low delay volumes.

Ultralow carryover – The 1290 Infinity II Multisampler is designed for low carryover, but you can take clean to a whole new level with our multi-wash capability, cleaning all relevant injection parts between runs. This sophisticated, integrated feature flushes the injection needle outside with three solvents, and uses seat backflush procedures to reduce carryover to less than 9 ppm.

Integrated sample thermostat – available as option or upgrade, providing cooling and heating in the range from 4 °C – 40 °C.

Instant information – Lights on each drawer tell you all you need to know about loading status, current activity, and accessibility.

Unmatched flexibility – You can choose how you want to introduce samples for injection, whether you prefer vials, microtiter plates, or any combination of formats. Sample drawers are available in three heights, and you can mix shallow drawers with deeper ones to accommodate different sample sizes.

High capacity – Using shallow well-plate drawers, the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates and up to 6144 samples-the most of any single system.

Seamless automation – Internal robotics move microtiter plates and other sample containers from the sample hotel to the central workspace for sample processing steps and injections.

Dual-needle injection – By running samples alternately through one or the other injection path, you can reduce cycle times to mere seconds, virtually eliminating conventional wait times-whether for large volume loadings or flushing procedures.

Scalable injection volumes – The Agilent dual-needle setup enhances flexibility by providing two differently optimized injectors in a single instrument. You can, for example, optimize one path for large volume injections and the other for low delay volumes.

Ultralow carryover – The 1260 Infinity II Multisampler has a low carryover, and a multi-wash capability, cleaning all relevant injection parts between runs. This integrated feature flushes the injection needle outside with three solvents, and uses seat backflush procedures to reduce carryover to less than 9 ppm.

Efficient temperature control – For temperature-sensitive samples, add Agilent’s compressor-based cooling system. It maintains temperature control on all vials and plates inserted into the 1260 Infinity II Multisampler.

Instant information – Lights on each drawer tell you about loading status, current activity, and accessibility.

Reliable analysis of biological samples – the metal-free sample flow path at 600 bar means that none of your precious sample touches metal surfaces.

Maintain perfect temperature control – for temperature-sensitive samples, simply add Agilent’s new highly efficient compressor-based cooling system. It allows you to efficiently control the temperature of all vials and plates inserted into the 1260 Infinity II Multisampler.

Ultralow carryover – the 1260 Infinity II Multisampler is designed for low carryover. But you can take clean to a whole new level with our multiwash capability, cleansing all relevant injection parts between runs. This sophisticated, integrated feature flushes the injection needle outside with three solvents, and uses seat backflush procedures to reduce carryover to less than 9 ppm.

Unmatched flexibility – you choose how you want to introduce samples for injection, whether you prefer vials, microtiter plates, or any combination of formats. Sample drawers are available in three heights, and you can mix shallow drawers with deeper ones to accommodate different sample sizes.

High capacity – using shallow well-plate drawers, the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates and up to 6144 samples. The most of any single system.

Seamless automation – internal robotics move microtiter plates and other sample containers from the sample hotel to the central workspace for sample processing steps and injections.

The Multisampler offers two main possibilities to reduce carry-over:

1. External Needle Wash (Standard configuration): The needle flush station uses a peristaltic pump to wash the outside of the needle with mobile phase from the solvent cabinet. This reduces low carry-over for sensitive analysis. Waste is channeled away through a drain.

2. Multi-Wash Configuration:

– External needle wash is performed by a micro piezo pump combined with a solvent selection valve, allowing selection between three different solvents.

– For even lower carry-over, an integrated high-pressure flush pump can perform seat backflushing. This pump can also select between three different solvents. It connects to port 4 of the injection valve (normally the waste line). When the Multisampler is in bypass mode, the flush pump flushes backwards through the needle seat into the waste line attached to the needle seat outlet port.

Additionally, in the standard configuration, the entire injection flowpath is always flushed by the mobile phase for minimum internal carry-over.

Temperature control is achieved using an optional Agilent Sample Cooler or Sample Thermostat module, which functions as a micro compressor-based refrigerator.

1. A fan draws air from the central workstation area above the sample container.

2. The air is blown through the cooling fins of the cooler/thermostat module, where it’s cooled according to the set temperature.

3. The cooled air enters the Sampler Hotel through a recess under the base plate.

4. The air is distributed evenly throughout the Sample Hotel, ensuring effective temperature control for all sample containers in the drawers.

In cooling mode, condensation forms on the cooled side of the Sample Cooler/Thermostat. This condensed water is guided into a dedicated waste bottle located underneath the working bench.

The standard sampling sequence occurs in the following order:

1. The robot loads the required sample container onto the central workspace.

2. The injection valve switches to the bypass position.

3. The plunger of the metering device moves to the initialization position.

4. The robot couples into the needle assembly from the needle park station.

5. The robot unlocks the needle assembly and moves up.

6. The coupled needle assembly/robot moves to the desired sample vial (or well plate) position on the central workstation.

7. The needle lowers into the sample vial (or well plate).

8. The metering device draws the preset sample volume.

9. The needle lifts out of the sample vial (or well plate).

10. The coupled needle assembly/robot is then moved to the park station onto the seat to close the sample loop. (If needle wash is required, it is done between step 9 and 10).

11. The needle assembly is locked into the park station and moves down.

12. The injection cycle is completed when the injection valve switches to the mainpass (main path) position.

13. The robot moves the sample container back into the sample hotel if the sampling sequence is done.

NOTE: For the needle seat backflush, the Multisampler must be in bypass mode. If an additional needle seat backflush is required, this step must also be done between step 5 and 9.

1. Mainpass (Ready/Run State):

– Before injection and during analysis, the valve is in the mainpass position.

– Mobile phase flows from the pump, through the valve (port 1 to 2), through the metering device, sample loop, needle/seat assembly, and then out through the valve (port 5 to 6) to the column.

– This flushes all sample-contacting parts during the run, minimizing carry-over.

2. Bypass (Drawing Sample):

– The sequence begins, and the valve switches to bypass.

– Mobile phase from the pump flows directly through the valve (port 1 to 6) to the column, bypassing the sampler components.

– The needle moves to the sample vial/plate, lowers into the liquid, and the metering device draws the set volume.

– The needle lifts and moves to the needle seat in the park station, closing the sample loop.

3. Flush Needle (Optional):

– Before returning to the seat, the needle can move to the flush port.

– The wash pump delivers solvent to clean the outside of the needle.

– The needle then moves to the needle seat.

4. Inject-and-Run (Mainpass):

– The valve switches back to the mainpass position.

– Mobile phase flow is directed through the sample loop (port 1 to 2, loop, needle/seat, port 5 to 6), transporting the drawn sample onto the column.

– Separation begins, and the system is flushed internally by the mobile phase flow.

5. Needle Seat Backflush (Optional – Multiwash in Bypass):

– After injection, the valve can switch back to bypass.

– An integrated flush pump delivers solvent (up to 3 selectable) through valve port 4, backflushing the needle seat.

– Waste exits via the needle wash port line.

– The valve returns to mainpass for the next run (often after a final rinse with mobile phase).

The dual needle system uses two flow paths (left and right) with separate sample loops and needles/seats, allowing one path to prepare while the other is running, minimizing cycle times.

1. Flushing the System (Initialization):

– Starting the pump or changing solvent composition triggers a purge routine.

– The hydraulic setup (metering device, sample loops, needles) is flushed with fresh mobile phase.

– Both valves are typically in the mainpass position initially.

– *Note: For pumps with manual purge valves, manually starting the purge routine before a run/sequence is mandatory.*

2. Prepare Inject and Run (e.g., Left Needle while Right is Running):

– The injection valve switches to the mainpass position for the *left* path (ports 2-1 and 5-6 connected).

– Mobile phase flows through the *left* loop and needle/seat to the column.

– Simultaneously, the robot moves a sample container to the workspace.

– The robot detaches the *right* needle assembly and moves it to the sample.

– The metering device draws the desired volume using the *right* needle.

– The *right* needle moves to its park station/seat, closing the *right* sample loop.

– (Optional) The *right* needle can be flushed externally before seating.

3. Inject and Run (e.g., Right Needle while Left Prepares):

– The eight-port valve switches to the mainpass position for the *right* path (ports 2-3 and 7-6 connected).

– Mobile phase flow is directed through the *right* sample loop, injecting the sample onto the column.

– Separation and analysis begin for the *right* sample.

– In the meantime, the *left* needle path starts its preparation sequence (drawing sample, etc.) as described in step 2, but for the left side.

4. Alternating Cycles:

– The system continues alternating between the left and right paths, preparing one while the other injects and runs.

Multi-load (for large volumes):

– The peripheral valve switches positions while the metering plunger moves back and forth, allowing multiple draws from the same vial/well without removing the needle, enabling large volume injections.

Bypass Mode:

– Similar to single needle bypass, one flow path is used regularly, while the other path is replaced by a bypass capillary, shortcutting it for faster reconditioning if only one path is needed.

The system is designed for safe leak and waste management:

Leak Handling:

– All modules feature a leak plane with outlets in a consistent position for stacking.

– Internal liquid leaks are caught by the leak plane.

– Leaks are guided to a leak sensor within the module.

– If the leak detection level is reached, the sensor stops the running system.

– If the sensor fails, leaks are passed down to the next module below.

– The waste tube from the leak pan outlet on the bottom-most instrument guides collected leak solvent to a suitable waste container.

Waste Handling:

– Waste tubes are guided through a channel on the right side of the instrument stack, keeping front access clear.

– Solvent waste and condensate are guided through the waste channel into the waste container.

– Sources typically include:

– Detector’s flow cell outlet

– Multisampler needle wash port (Standard or Multi-Wash)

– Sample Cooler or Sample Thermostat condensate

– Pump’s Seal Wash Sensor outlet (if applicable)

– Pump’s Purge Valve or Multipurpose Valve outlet

Solvent Cabinet:

– Designed to store a maximum of 8 L of solvent.

– Individual bottle volume should not exceed 2 L.

Mixed Configurations:

– Ensure the leak pan outlet of an upper module is vertically positioned above the leak tray of the lower module.

– For the Multisampler, flush solvent from the washport is guided out to the right of the instrument.

CAUTION: Solvent incompatibility

The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.

✓ Do not use DMF as mobile phase.

✓ Check the leak sensor regularly for corrosion.

The waste drainage must go straight down into the waste containers. The waste flow must not be restricted at bends or joints. Ensure the end of the tube does not immerse in the liquid waste.

Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (part number 5043-1221) for optimal and safe waste disposal. If you use your own waste solution, ensure that the waste tubes do not immerse in the liquid.

No. Do not place the multisampler directly on the bench if a sample cooler or sample thermostat is installed. This is likely due to the need for space underneath for condensate drainage from the cooling unit.

Power Supply:

– The module accepts a wide range of line voltages (see Physical Specifications table for exact range, typically 100-240 V~).

– There is no external voltage selector.

– Internal automatic electronic fuses are used; no externally accessible fuses.

Safety Warnings:

– Voltage: Connect the instrument only to the specified line voltage. Connecting to a higher voltage can cause electrical shock or damage.

– Partial Power: The module is partially energized when switched off as long as the power cord is plugged in. Do not open the cover to avoid electrical shock.

– Damaged Cover: Do not operate the instrument if the cover shows signs of damage. Disconnect the power cable and contact Agilent support.

– Emergency Disconnect: The power plug must be accessible at all times for emergency disconnection. Ensure the power connector is easily reachable and provide sufficient space behind the instrument to unplug the cable.

– Power Cords:

– Use only the power cord shipped with the instrument by Agilent.

– Do not use Agilent power cords for other equipment.

– Use only cables supplied by Agilent to ensure proper function and safety/EMC compliance.

– Grounding: Never operate the instrumentation from a power outlet without a ground connection to avoid electric shock or short circuits.

– Solvent Damage: Prevent electrical cables from contacting solvents. Exchange cables immediately if contact occurs.

Room Size and Ventilation (especially with Sample Thermostat):

– WARNING: Flammable Refrigerant (R600a in Sample Thermostat)

– Keep open flames or ignition sources away from the device.

– Ensure a minimum room size of 4 m³ (or 1 m³ for every 8 g of R600a refrigerant inside the thermostat).

– Ensure adequate ventilation: typical air exchange of 25 m³/h per m² of laboratory floor area.

– Keep all ventilation openings on the instrument enclosure clear of obstructions. Do not block openings on the circumference of the Sample Thermostat.

Bench Space:

– Refer to the Physical Specifications table for module dimensions and weight.

– Requires an additional 2.5 cm (1.0 inch) of space on either side.

– Requires approximately 8 cm (3.1 inches) of space in the rear for air circulation and connections.

– Ensure the bench can support the weight of all modules in the HPLC system.

– Operate the module horizontally, especially if a Sample Cooler/Thermostat is installed (use a bubble level to check).

– Agilent recommends using the InfinityLab Flex Bench rack to save space and facilitate relocation.

WARNING: Heavy weight

The module is heavy.

✓ Carry the module with at least 2 people.

✓ Avoid back strain or injury by following all precautions for lifting heavy objects.

✓ Ensure that the load is as close to your body as possible.

✓ Ensure that you can cope with the weight of your load.

CAUTION: Condensation within the module

Condensation can damage the system electronics.

✓ Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module.

✓ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.

¹ If a sample thermostat is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non-condensing operation.

¹ If a sample thermostat is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non-condensing operation.

¹ If a sample thermostat is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non-condensing operation.

The performance specifications are detailed in the table below:

The physical specifications for the Agilent Infinity II Sample Cooler are:

Note: The Agilent Infinity II Sample Cooler uses a fluorinated greenhouse gas (HCF-134a) as the refrigerant. For information on carbon dioxide equivalency (CDE) and global warming potential (GWP), see the instrument label.

Improper disposal of the media and components used pollutes the environment.

The disposal or scrapping of the Sample Cooler or the Sample Thermostat must be carried out by a qualified disposal company.

All media must be disposed of in accordance with national and local regulations.

Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check http://www.agilent.com for more info.

The performance specifications for the Agilent Infinity II Sample Cooler are:

Note: The Agilent Infinity II Sample Cooler is not available for trade sales anymore and has been replaced by the Agilent InfinityLab Sample Thermostat.

No, the Agilent Infinity II Sample Cooler is not available for trade sales anymore and has been replaced by the Agilent InfinityLab Sample Thermostat.

The physical specifications for the Agilent InfinityLab Sample Thermostat are:

The Agilent InfinityLab Sample Thermostat is a combination of an electric heater and a vapor-compression refrigeration system. It uses isobutane (R600a) as a non-Freon refrigerant, which is harmless to the environment and does not affect the ozone layer and global warming but is combustible. Please adhere to the warnings listed in the manual.

The Agilent InfinityLab Sample Thermostat uses Isobutane (R600a) as the refrigerant (0.030 kg). It is a non-Freon refrigerant that is harmless to the environment (ODP=0, GWP=3) but is combustible.

The performance specifications for the Agilent InfinityLab Sample Thermostat are:

Minimum firmware revision for the Sample Thermostat is D.07.22.

Minimum LC driver revision for the Sample Thermostat is A.02.14.

Magnets are located in the door of the multisampler and in the drawers of the multisampler.

This procedure shows an arbitrary LC stack configuration:

1. Locate the power switch on the front of the module(s).

2. To turn On: Ensure the power switch is pressed in (On position).

3. To turn Off using software: Use the On/Off button in the control software interface (e.g., Instrument Idle > Off).

4. To turn Off using power switch: Press the power switch so it stands out (Off position).

The module status indicator (typically a light bar) shows one of six possible module conditions:

1. Idle (e.g., green light)

2. Run mode (e.g., darker green/different color light)

3. Not-ready (e.g., yellow light): Waiting for a specific pre-run condition to be reached or completed.

4. Error mode (e.g., red light): Interrupts the analysis and requires attention (for example a leak or defective internal components).

5. Resident mode (blinking, e.g., yellow blinking): For example, during update of main firmware.

6. Bootloader mode (fast blinking, e.g., red fast blinking): Try to re-boot the module or try a cold-start. Then try a firmware update.

The module drawer status indicator (circular indicator near the drawers) indicates one of three possible module conditions:

• When the status indicator is OFF: No sample containers are loaded.

• When the upper, lower or both semi-circle status indicators are ON: Indicates the rear or front position of the drawer or both positions are loaded with sample containers.

• When semi-circle indicators are blinking: The robot interacts with a drawer.

During blinking of the drawer status indicator, do not try to open the drawer at this point, as the robot is interacting with it.

1. Pull the drawer out.

2. Place the vial tray or wellplate into the drawer.

3. Push the drawer back in partially.

4. Check the orientation of the vial tray/wellplate (e.g., position A1 towards the front) and ensure correct seat by pressing down on the plate. When the lever sensor has detected the plate correctly, the front LED lights up and the device recognizes the assignment.

5. Push the drawer completely closed.

6. The corresponding drawer status indicator LED should light up.

7. Configure the vial tray/wellplate type in the chromatographic data system.

1. Ensure the system is idle and the drawer is not being accessed by the robot (indicator not blinking).

2. Pull the drawer handle to open the drawer fully.

3. Lift the vial tray or wellplate out of the drawer.

4. Push the drawer closed.

If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment.

If there are signs of damage, please do not attempt to install the module. Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged.

Notify your Agilent sales and service office about the damage.

An Agilent service representative will inspect the instrument at your site and initiate appropriate actions.

Ensure that all parts and materials have been delivered with your module. Please report any missing or damaged parts to your local Agilent Technologies sales and service office.

Note: The Agilent Infinity II Sample Cooler is not available for trade sales anymore and has been replaced by the Agilent InfinityLab Sample Thermostat.

Tools required:

• Screwdriver Pozidrive Shaft (p/n 8710-0899) (for the Sample Cooler)

• Torx screwdriver T10 (p/n 5182-3466) (for the Sample Thermostat) OR Torx key set (p/n 5023-3089)

Parts required:

• 1 x Multisampler

• 1 x Sample Cooler (G7167-60005) OR Sample Thermostat (G7167-60101)

• 1 x Power cord

• 1 x Installation of the Infinity II Cooler/Thermostat Condensate Drainage Tubing Kit (G7167-90171)

Preparations:

• Sampler is installed in the stack.

WARNING: Flammable refrigerant

Formation of flammable gas-air mixtures inside the Sample Thermostat and laboratory.

✓ Keep open fire or sources of ignition away from the device.

✓ Ensure a room size of 4 m³ (1 m³ for every 8 g of R600a refrigerant inside of the Sample Thermostat).

✓ Ensure adequate ventilation: typical air exchange of 25 m³/h per m² of laboratory floor area.

✓ Keep all ventilation openings in the enclosure clear of obstructions. Do not block the openings on the circumference of the Sample Thermostat.

WARNING: Flammable refrigerant used

When handling, installing and operating the Sample Thermostat, care should be taken to avoid damage to the refrigerant tubing or any part of the Sample Thermostat.

CAUTION: Condensate inside the Sample Cooler/Sample Thermostat

Damage to the electronics of the module.

✓ After installation of the Sample Cooler/Sample Thermostat, wait at least 30 min before switching on the module.

✓ Make sure there is no condensate inside the module.

CAUTION: Damage to the Sample Cooler/Sample Thermostat

✓ Wait at least 30 min before switching on the compressor of the cooler/thermostat. This allows the refrigerant and system lubrication to reach equilibrium.

WARNING: In the event of a damage

✓ Keep open fire or sources of ignition away from the device.

✓ Ventilate the room for several minutes.

✓ Do not use the Sample Thermostat any more.

✓ Disconnect it from the power supply and call your local service center.

Do not open the Sample Thermostat. There are no serviceable parts inside.

If the sample cooler or thermostat is disconnected from the power supply, you should wait for at least five minutes before replugging and switching on the compressor again.

Even under average humidity conditions, a significant amount of condensed water gathers every day. A suitable container must be provided and emptied regularly in order to avoid overflow.

Depending on the ambient conditions in the lab, the amount of condensate can vary from 200 mL to 2 L per day. Do not fill waste containers for the condensate to the top. Regularly empty the waste container.

For best cooling performance of the thermostat, the 2H drawer must be installed in the lowest position. Use the dummy drawers (G4267-60024) if no full hotel configuration is needed.

WARNING

Module is partially energized when switched off, as long as the power cord is plugged in.

Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened and the module is connected to power.

✓ Make sure that it is always possible to access the power plug.

✓ Do not use the Sample Cooler/Sample Thermostat if it is not operating correctly or has been damaged. Disconnect it from the power supply and call your local service center.

✓ Remove the power cable from the module before opening the cover.

✓ Do not connect the power cable to the module while the covers are removed.

✓ If the Sample Cooler/Sample Thermostat is disconnected from the power supply, you should wait for at least five minutes before switching on the compressor.

CAUTION: Damaged electronics

✓ To avoid damages of the electronics of the module make sure the power cords are unplugged before disconnecting or reconnecting the sampler to the Sample Cooler/Sample Thermostat cables.

To ensure adequate drainage for condensate, the module should be operated in a proper horizontal position. Use a bubble level to check the leveling of the sampler during installation.

Adhere the tubing holder clamp to the side of the cooler/thermostat where the drain pipe is situated. Ensure a distance of 60 mm from the bottom edge.

1. Mount the condensate tubing assembly with the y-connector on the drainpipe.

2. Fix the venting tube in the tubing holder clamp (positioned 60 mm from the bottom edge, venting tube should extend approx. 100 mm above clamp).

3. Ensure the drainage tubing runs straight into the waste container without any bends or joints.

4. Ensure the free end of the drainage tube does not immerse in the liquid in the waste container.

5. The condensate handling system should be installed in a way that it allows a continuous slope for the drained liquid. Horizontal or uphill sections may hinder the drainage.

For more information, refer to “Leak and Waste Handling”.

Ensure preparations and safety warnings/cautions are followed.

1. Ensure the power switch on the front of the sampler module is OFF (switch stands out).

2. Disconnect the power cable from the sampler.

3. Loosen the four screws on the rear of the sampler module.

4. Remove the sheet metal back cover of the sampler.

5. Slide the Sample Cooler/Sample Thermostat halfway into the sampler.

6. Connect the power cable and the data cable from the sampler to the cooler/thermostat. Ensure power cords are unplugged during connection.

7. Carefully slide the Sample Cooler/Sample Thermostat all the way into the sampler. Do not bend or pinch the cables. Ensure it fits perfectly.

8. Fix the Sample Cooler/Sample Thermostat with the four screws loosened in step 3.

9. Use a bubble level to check the leveling of the sampler. Ensure it is operated in a proper horizontal position for condensate drainage.

10. Adhere a tubing holder clamp to the side of the cooler/thermostat (drain pipe side), 60 mm from the bottom edge.

11. Mount the condensate tubing assembly with the y-connector on the drainpipe and fix the venting tube in the tubing holder clamp.

12. Ensure the drainage tubing runs straight to waste, without bends/joints, and the end is not immersed in liquid. Ensure a continuous downward slope.

13. Connect the main power cable to the power connector at the rear of the sampler module.

14. Wait at least 30 minutes before switching on the compressor.

15. Configure the Sample Cooler/Sample Thermostat in the Chromatography Data System (CDS).

The status indicator of the Sample Cooler/Sample Thermostat is incorporated in the graphical user interface (GUI) of the hosting sampler. It appears automatically when the unit is configured in the chromatography data system (CDS).

When the cooler/thermostat is turned on, the set temperature and the actual temperature are also displayed on the dashboard, along with a status indicator (On/Off).

• Item 8: Cooler/Thermostat Status indicator (Off)

• Item 9: Cooler/Thermostat Status indicator (On)

• Item 10: Cooler/Thermostat Set temperature

• Item 11: Cooler/Thermostat Actual temperature

If the actual temperature differs by more than ± 2 °C from the set temperature, a yellow highlight is visible around the temperature reading. This, however, will not prevent the system from starting a new analysis, unless the ‘Enable Analysis > Temperature within +/- 2 °C’ function is selected (available for Sample Thermostat only).

Note: The actual temperature may deviate from the set temperature by up to 3 °C, depending on the temperature setting and ambient conditions.

Right-clicking the sampler GUI (dashboard) will prompt the control interface. From here, control and method parameters can be edited, configuration modified (e.g., Modify > Temperature Mode), and special commands executed.

With the Sample Cooler/Sample Thermostat installed, the Control dialog box of the hosting sampler includes these specific options:

• At Power On:

• Turn On Thermostat: The cooler/thermostat turns on automatically upon powering on the sampler.

• Thermostat:

• On: The cooler/thermostat turns on and the system starts to regulate the temperature inside the sample space towards the setpoint.

• Off: The cooler/thermostat turns off.

• Enable Analysis (available since LC & CE drivers A.02.19):

• With any temperature: The analysis starts regardless of the actual temperature inside the sampler.

• Temperature within +/- 2 °C: The analysis starts only when the actual temperature is within the ± 2 °C range of the setpoint temperature. (Note: This option is only available for the Sample Thermostat).

For the Sample Cooler, the set temperature must be at least 5 °C below ambient for proper temperature control.

The temperature control mode can be switched between being a method parameter or a system (control) setting. This is done by selecting Modify > Temperature Mode in the Control Interface (requires LC & CE drivers A.02.12 or higher).

• Constant Temperature Mode: The temperature control mode is defined as a system (control) setting. The temperature setting is independent of the method parameters and stays constant for all methods within a given sequence. This is the default option and recommended for most applications.

• Variable Temperature Mode: The temperature control mode is defined as a method parameter. The temperature setting is part of the method parameters, allowing the temperature to change from method to method within a given sequence. This mode is not recommended for most analytical workflows but might be used for special applications like degradation studies.

Before using the Variable Temperature Mode setting, consider the following:

• Changing the temperature setting from one method to another will affect all samples inside the sampler.

• Depending on the extent of the temperature change (e.g., from 4 to 40 °C or vice versa), it could take up to a couple of hours until the sample temperature stabilizes at the new setpoint.

• It might be beneficial to use the ‘Temperature within +/- 2 °C’ function (available for Sample Thermostat); otherwise, the next run will start without waiting for the new setpoint being reached.

In the Online Signals tab of the Chromatography Data System (CDS), the actual temperature of the sample space can be configured and plotted together with other instrument actuals. This allows you to have a better overview of how the temperature changes over time.

The actual and setpoint temperature can be included in the analysis report. To do this, the ‘Samples > Advanced Run Information’ field must be included in the report template.

For example, in OpenLab CDS 2.4, you would add this field to your template. The report would then show values like “Run start – Temperature” and “Run start – Temperature setpoint”.

Note: For OpenLab CDS ChemStation, this option is only available in Intelligent Reporting.

Depending on the ambient conditions and the sampler configuration (e.g., hotel configuration for the Multisampler), reaching the setpoint temperature can take from 30 minutes up to a couple of hours.

As an example, reaching the 4 °C setpoint from an ambient temperature of 22 °C takes about 45 minutes for the Vialsampler (G7129A/B/C or G7157A), as well as for the Multisampler (G7167A/B, G5668A, or G4767A) with a single 2H drawer installed.

This relatively slow ramping down of the temperature is necessary to avoid ice formation.

For the best performance of the Sample Cooler/Sample Thermostat, all drawers must be installed in the sampler. For the Multisampler, use dummy drawers if no full hotel configuration is needed.

Operating the cooler/thermostat at temperatures below ambient results in condensate formation, which is collected and drained. If the container for condensate collection is overfilled or the condensate tubing is blocked, the condensate sensor is triggered, rendering the HPLC system to enter the error state.

Setting the cooler/thermostat from a lower to a higher temperature setpoint, or just simply turning it off, can result in dew formation on the internal surfaces of the sampler. This is normal and should cease after a couple of hours at the most.

Opening the door(s) and/or the sample drawers frequently can compromise the temperature stability, as fresh warm and humid air will enter each time. In a highly humid environment, this could also lead to the formation of significant amounts of condensate on the internal surfaces of the sampler.

The Sample Cooler/Sample Thermostat was designed to operate without the risk of icing. In an unlikely event of ice formation, turn off the cooler/thermostat and wait until it defrosts.

Do not use mechanical devices or other means to accelerate the defrosting process.

When the Sample Cooler/Sample Thermostat needs to be turned off for the night or a longer period, the following best practices are recommended:

• Remove all sample containers and/or vials from the sampler.

• Let the system reach the ambient temperature. Opening the door(s) of the sampler facilitates this process.

• Remove any condensate that might appear on the sample drawers or the internal surfaces of the sampler.

• Make sure that all condensate is removed from the cooler/thermostat. Gently tapping on the sides of the sampler facilitates condensate removal. Tilting the module towards its right back corner is not recommended as it can damage the internal parts.

WARNING: Heavy weight

The module is heavy.

✓ Carry the module at least with 2 people.

✓ Avoid back strain or injury by following all precautions for lifting heavy objects.

✓ Ensure that the load is as close to your body as possible.

✓ Ensure that you can cope with the weight of your load.

CAUTION: Unsecured transportation

Mechanical damage

✓ Secure the transport assembly before transporting the sampler.

When the module needs to be transported or relocated:

1. Remove all sample containers from the sample hotel.

2. Move the robot arm to the park position using Instant Pilot or Lab Advisor (see “Arm Position” section).

3. Turn off the sampler.

4. Install all parts of the Transport Protection, see “Install the Transport Protection Foam”.

Ensure lifting and transport safety precautions are followed.

When the module with an installed Cooler/Thermostat needs to be transported or relocated:

1. Follow all heavy weight lifting precautions (carry with at least 2 people, proper technique).

2. Prepare for Condensate:

CAUTION: Condensate inside the cooler or thermostat (Damage to the electronics)

✓ Unplug the power cords.

✓ Drain off all condensate before dismounting the sample cooler or thermostat (see procedure below).

✓ Make sure that there is no condensate left.

3. Remove Condensate:

a. Place a suitable container underneath the outlet pipe.

b. Remove the drainage tube.

c. Gently tap the sides of the sampler several times to facilitate the drainage of the condensate from the system. Do not tilt the module to avoid damage to the internal parts.

4. Remove all sample containers from the sample hotel.

5. Move the robot arm to the park position using Instant Pilot or Lab Advisor.

6. Turn off the sampler.

7. Install all parts of the Transport Protection foam.

8. Consider Removal: Moving the sampler with the Cooler/Thermostat installed is possible for short distances (e.g., workbench to workbench). For longer transportation, remove the cooler/thermostat from the sampler and handle the units separately (see “Replace the Sample Cooler/Sample Thermostat” section).

9. Secure for Transport:

CAUTION: Unsecured transportation (Mechanical damage)

✓ Secure the transport assembly before transporting the sampler.

This procedure secures the transport arm before transporting or shipping the sampler.

Parts required: Transport Protection (p/n G4267-40033)

Preparations:

• All sample containers are removed from the sample hotel.

• Module is switched off.

Procedure:

1. Open the front door.

2. Manually move the robot arm to the right back corner of the lobby (park position).

3. Carefully slide the right protection foam piece into the sampler.

4. Position the right foam next to the hydraulic box such that it sits behind the needle port.

5. Slide the left protection foam piece into the sampler.

6. Firmly push the left foam piece into place until it snaps in behind the hotel support frame.

7. Place the protection foam for the hydraulic box in a way that it snaps onto the analytical head.

8. Verify that all three protection foams are in the correct position.

9. Close the front door.

Observe the following recommendations on the use of solvents:

• Follow the recommendations for avoiding the growth of algae, see the pump manuals.

• Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 µm filters.

• Avoid or minimize the use of solvents that may corrode parts in the flow path. Consider specifications for the pH range given for different materials such as flow cells, valve materials etc. and recommendations in subsequent sections.

Recommended Wash Solvents:

• water

• ethanol

• methanol

• water/acid (especially for basic compounds)

• water/base (especially for acidic compounds)

• water/acetonitrile

Note: For different wash solvents as mentioned above, verify that the wash solvent is suitable for the silicone wash tubing.

The table shows the chemical resistance properties of Silicone and PharMed tubing to different needle wash solvents:

For the Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (wetted parts). These materials are widely accepted by life science scientists for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.

Explicitly, the complete flow path is free of stainless steel and free of other alloys containing metals such as iron, nickel, cobalt, chromium, molybdenum or copper, which can interfere with biological samples.

The flow path downstream of the sample introduction contains no metals whatsoever.

To ensure optimum bio-compatibility of your Agilent 1260 Infinity II Bio-inert LC system, do not include non-inert standard modules or parts to the flow path. Do not use any parts that are not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see “Material Information”.

PEEK (Polyether-Ether Ketones) combines excellent properties regarding biocompatibility, chemical resistance, mechanical and thermal stability.

It is stable in the specified pH range (for the Bio-inert LC system: pH 1 – 13, see bio-inert module manuals for details), and inert to many common solvents.

Known incompatibilities include: chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %, sulfuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogens or aqueous halogen solutions, phenol and derivatives (cresols, salicylic acid, and so on).

When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions, normal PEEK capillaries are very sensitive to high pressure. Agilent uses stainless steel cladded PEEK capillaries in bio-inert systems to maintain a steel-free flow path while ensuring pressure stability up to at least 600 bar.

If in doubt, consult the available literature about the chemical compatibility of PEEK.

Agilent uses semi-crystalline polyimide (e.g., DuPont Vespel) for rotor seals in valves and needle seats.

Polyimide is stable in a pH range between 1 and 10 and in most organic solvents.

It is incompatible with concentrated mineral acids (e.g., sulphuric acid), glacial acetic acid, DMSO and THF.

It is also degraded by nucleophilic substances like ammonia (e.g., ammonium salts in basic conditions) or acetates.

Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow piston and wash seals in certain pumps.

Polyethylene has good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5.

It is compatible with many organic solvents used in chromatography like methanol, acetonitrile and isopropanol.

It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases.

For normal phase applications, the maximum pressure should be limited to 200 bar.

Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide.

It can be corroded by strong bases (e.g., hydroxide solutions > 10 %, diethylamine).

It is not recommended for the use with fluoric acid and fluorides.

Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5.

It can be corroded by acids below pH 2.3.

It can also corrode in the following solvents/conditions:

• Solutions of alkali halides, their respective acids (e.g., lithium iodide, potassium chloride) and aqueous solutions of halogens.

• High concentrations of inorganic acids (nitric acid, sulfuric acid) and organic solvents, especially at higher temperatures. (Phosphoric acid or phosphate buffer are less corrosive alternatives).

• Halogenated solvents or mixtures which form radicals and/or acids (e.g., unstabilized chloroform forming HCl).

• Chromatographic grade ethers containing peroxides (e.g., THF, dioxane, diisopropylether). Filter through dry aluminum oxide to remove peroxides.

• Solutions of organic acids (acetic acid, formic acid) in organic solvents (e.g., 1 % acetic acid in methanol).

• Solutions containing strong complexing agents (e.g., EDTA, ethylene diamine tetra-acetic acid).

• Mixtures of carbon tetrachloride with 2-propanol or THF.

Titanium is highly resistant to oxidizing acids (e.g., nitric, perchloric, hypochlorous acid) over a wide range of concentrations and temperatures due to a stabilizing thin oxide layer.

Non-oxidizing acids (e.g., hydrochloric, sulfuric, phosphoric acid) can cause slight corrosion, increasing with concentration and temperature (e.g., ~13 µm/year with 3% HCl at room temp; resistant to ~5% sulfuric acid at room temp).

Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates.

Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2.

Titanium is subject to corrosion in anhydrous methanol; adding a small amount of water (~3 %) avoids this.

Slight corrosion is possible with ammonia > 10 %.

Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fused silica and Quartz are used in Max Light Cartridges and classical flow cell windows respectively.

They are inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides.

They are corroded by strong bases and should not be used above pH 12 at room temperature. Corrosion of flow cell windows can negatively affect measurement results.

For a pH greater than 12, the use of flow cells with sapphire windows is recommended.

Gold is inert to all common HPLC solvents, acids and bases within the specified pH range.

It can be corroded by complexing cyanides and concentrated acids like aqua regia.

Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents.

FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons.

TFE/PDD copolymer tubings, used in all Agilent degassers except G1322A/G7122A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel.

They also have limited life time in the presence of Hexafluoroisopropanol (HFIP).

To ensure the longest possible life with HFIP when using degassers with TFE/PDD tubing, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let the chamber dry out. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.

The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethyl formamide).

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.

In some cases the multisampler has to be reset by the user in order for the system to resume working in normal operation mode.

WARNING: Risk of injury by uncovered needle

An uncovered needle is a risk of harm to the operator.

✓ Open the safety lock of the needle assembly only on the sample handler and for this particular procedure.

✓ Be careful working at the z-robot.

✓ Wear safety gloves when removing the needle assembly.

Follow all safety warnings regarding the uncovered needle.

1. Check the condition of the needle assembly and the sample loop. Replace them if necessary. Ensure the needle is installed properly (plastic adapter correct, loop not kinked).

2. Unlock the needle: This procedure is different from standard PM replacement. The safety lock of the needle assembly has to be released by carefully sliding the pusher upwards.

3. Verify that the needle assembly is unlocked after installation.

4. Reset the multisampler using the instrument control software (e.g., right-click menu > Reset Injector) OR turn the instrument Off and On again to start the initialization.

Next Steps:

5. Close the front door.

6. Wait until the initialization of the multisampler is completed.

7. If the error persists, contact your local service representative.

The Agilent InfinityLab Companion G7108AA provides complete local control, system monitoring, signal plotting, and diagnostic capabilities for a wide range of LC system modules.

It combines the conveniences of the Agilent Instant Pilot with state-of-the-art mobile technology, offering maximum flexibility and ease of use for controlling and monitoring LC systems.

It is available as a full package (including hardware/accessories like a preconfigured tablet and holder) or can be used with your own mobile devices (tablets, phones).

• Complete local control and monitoring of Agilent Infinity II Prime LC Modules.

• Excellent usability via a user interface tailored for mobile devices (simple, intuitive, touch-enabled, visual).

• High flexibility through a modern “Bring your own device” approach.

• Connection between LC module and mobile device either wirelessly via WLAN or wired over USB cable (with full package).

• Convenient, ergonomic operation: handheld or attached to a module at the stack with a secure tablet holder (included in the full package).

• Optional preconfigured tablet with all required software already installed (included in the full package).

• Centerpiece is a USB dongle that activates the complete intelligence on the instrument stack.

The InfinityLab Companion provides:

• fast and direct control in front of the instrument

• a clear overview of the system status

• control functionalities

• access to method parameters and sequences

• a logbook showing events from the modules

• diagnose tests

The Agilent 1200 Infinity Series Instant Pilot controller (G4208A) offers complete control, system monitoring, signal plotting, and diagnostic capabilities for LC systems. Key features include:

• Complete local control and monitoring of an Agilent 1200 Series, 1260 Infinity, and 1290 Infinity system or a single module from a single point (Note: Not for Agilent 1220 Compact LC).

• Support for mixed system configurations, including 1200 Series, 1200 Series SL-, and 1100 Series.

• Excellent readability and usability due to a large colored display with background light, high resolution, and contrast.

• Convenient, ergonomic operation, either handheld or securely attached at the stack.

• Flexibility to be used handheld or attached to a module in a stack to suit operator preferences.

The 1200 Infinity Series Instant Pilot provides several capabilities to enhance LC system operation:

• Easy automation: Features like recalibration intervals and multimethod sequences cater to stringent automation routines.

• Data management: Allows transfer and archiving of methods, sequences, and logbooks using standard USB memory sticks.

• User-friendly software: Factory-installed software with a flat dialog structure, user-configurable interface, and an enhanced sequence engine (e.g., wait for baseline stabilization, diagnosis with passed/failed results).

• GLP compliance: Includes System logbook and module logbooks that record errors, unusual events, and maintenance activities for Good Laboratory Practice (GLP) traceability.

Handling solvents, samples, and reagents involves health and safety risks. Observe the following precautions:

✓ Adhere to appropriate safety procedures (e.g., wearing goggles, safety gloves, protective clothing) as outlined in vendor-supplied material handling and safety data sheets, and follow good laboratory practice.

✓ Do not use solvents with an auto-ignition temperature below 200 °C (392 °F).

✓ Do not use solvents with a boiling point below 56 °C (133 °F).

✓ Avoid high vapor concentrations. Keep the solvent temperature at least 40 K below its boiling point. This applies to the sample compartment as well. For methanol and ethanol, maintain the temperature at least 25 K below their boiling points.

✓ Do not operate the instrument in an explosive atmosphere.

✓ Do not use solvents of ignition Class IIC according to IEC 60079-20-1 (e.g., carbon disulfide).

✓ Minimize the volume of substances used for analysis.

✓ Never exceed the maximum permissible solvent volume of 8 L in the solvent cabinet. Use bottles within the maximum permissible volume specified in the solvent cabinet usage guideline.

✓ Ensure the waste container is grounded.

✓ Regularly check the waste container’s filling level to ensure sufficient free volume remains to collect waste liquid.

✓ To maintain maximum safety, regularly inspect tubing for correct installation.

NOTE: For detailed information on solvent cabinet usage and correct installation, refer to the usage guideline shipped with the solvent cabinet (also available online) and separate installation documentation.

For optimal multisampler performance:

• If using the multisampler with a system that has a vacuum degassing unit, briefly degas your samples before use.

• Filter samples prior to use in an InfinityLab LC Series system. Consider using an inline High pressure filter kit (5067-4638).

• When using buffer solutions, flush the system thoroughly with water before switching it off.

• When changing the piston seal, inspect the multisampler plungers for scratches, grooves, or dents. Damaged plungers can cause micro leaks and shorten the seal’s lifespan.

• Follow recommendations for solvent use as detailed in the “Solvent Information” section.

• Prime and purge the system if solvents have been exchanged or if the system has been off for a period (like overnight), as oxygen can re-diffuse into the solvent channel. This is necessary before starting an application.

The choice of priming solvent depends on the task:

The following plates and closing mats are recommended:

Closing Mat Compatibility Notes:

1: Closing mat for all 96 Agilent plates (5042-1389)

2: Mat 96 wells, square, pierceable, silicone 50/pk (5043-9319)

3: Mat 96 wells, round, pierceable, silicone 50/pk (5043-9317), Mat 96 wells, round, piercable, silicone 100/pk (5043-9318)

4: Mat 384 wells, square, pierceable, silicone 50/pk (5043-9320)

The following vial plates are recommended:

Please consider the following notes for vials and wellplates:

• For good chromatographic results, the maximum filling should not exceed 3/4 of the total volume of the vial.

• Agilent Technologies recommends using preslit septa.

• Bottom sensing detects the depth of vials or plates. If used, the bottom material must resist the needle. Ensure the material supports this feature.

• For the Needle height position setting, an offset of 0 equates to 2 mm above the wellplate bottom.

• When using custom-made wellplates or vials, respect the physical height limitations of each drawer type:

• 1H: 19 mm

• 2H: 45 mm

• 3H: 50 mm

(Maximum total height includes sample container and vial caps, if present).

• Adhesive foils are not recommended for sealing wellplates. As an alternative, plates can be sealed with a Pierceable aluminium foil (06644-001).

If the plate you are using is not found in the “Recommended Mats and Vials” list, you can configure it as a custom plate. Measure the exact dimensions of the plate according to the diagrams provided (Figures 19 for straight and 20 for staggered layouts) and enter these values into the plate configuration table within the ChemStation software. Refer to the Well Plate Dimensions table for definitions of each required measurement.

The following dimensions are required to define a custom well plate in the configuration software:

NOTE: These distances must be measured with high precision. Using calipers is recommended.

The capillary description uses keys for Type, Material, and Fittings (Left/Right).

Type Key: Indicates primary function.

• Capillary: Connection capillaries
• Loop: Loop capillaries
• Seat: Autosampler needle seats
• Tube: Tubing
• Heat exchanger: Heat exchanger

Material Key: Indicates raw material.

• ST: Stainless steel
• Ti: Titanium
• PK: PEEK
• FS/PK: PEEK-coated fused silica*
• PK/ST: Stainless steel-coated PEEK**
• PTFE: PTFE
• FS: Fused silica

*Fused silica in contact with solvent
**PEEK in contact with solvent

Fitting Left/Fitting Right Key: Indicates fitting type on each end.

• W: Swagelok + 0.8 mm Port id
• S: Swagelok + 1.6 mm Port id
• M: Metric M4 + 0.8 mm Port id
• E: Metric M3 + 1.6 mm Port id
• U: Swagelok union
• L: Long
• X: Extra long
• H: Long head
• G: Small head SW 4 mm
• N: Small head SW 5 mm
• F: Fingertight
• V: 1200 bar
• B: Bio
• P: PEEK

At-a-glance color-coding keys for Agilent capillary tubing ID:

Tip: Use narrower ID tubing for smaller-volume, high-efficiency columns, and wider ID tubing for conventional HPLC instruments.

For correct installation of capillary connections, it’s important to choose the correct fittings (refer to the “Capillary Color Coding Guide”). Example capillaries include ST 0.12 mm x 150 mm SL/SX (5067-4650), ST 0.12 mm x 280 mm SL/SX (5067-4651), ST 0.17 mm x 150 mm SL/SX (5067-4720), ST 0.17 mm x 280 mm SL/SX (5067-4722). You will also need long fitting screws (5065-4454, 10/pk). The quantity needed depends on your module configuration.

Follow these steps:

1. Select a nut that is long enough for the fitting being used.

2. Slide the nut over the end of the tubing or capillary.

3. Carefully slide the ferrule components onto the capillary after the nut.

4. Finger-tighten the assembly while ensuring the tubing is completely seated in the bottom of the end fitting.

5. Use a column or injection valve port to gently tighten the fitting. This action forces the ferrule to seat onto the tubing or capillary.

NOTE: Do not overtighten. Overtightening will shorten the lifetime of the fitting.

6. Loosen the nut and verify that the ferrule is correctly positioned on the tubing or capillary (it should grip the capillary).

NOTE: The first time a Swagelok fitting is used on a column or injection valve, the ferrule’s position is permanently set. If this fitting is moved to a different column or valve port, it might leak or negatively impact separation quality by contributing to band broadening.

Preparations:

• Ensure the module is installed in the system.

• Use an appropriate wash solvent based on your sample and mobile phase chemistries. The wash solvent should be the most solubilizing compatible solvent (strongest diluent). Selecting the right wash solvent is part of method development.

• A mixture of 50% up to 100% organic solvent in distilled water is often a good choice.

NOTE: The standard silicone waste drainage tubing is suitable for most common wash solvents. For critical wash solvents, consider replacing it with a PE tube (Tubing, PE, 1.5 m, 5042-9974).

Steps:

1. Place a needle wash solvent reservoir into the solvent cabinet.

2. Connect the Needle Wash Bottle Head Assembly to the solvent reservoir and close the bottle.

3. Guide the tube from the Needle Wash Bottle Head Assembly through the cover opening and connect it to the peristaltic pump.

4. Route the drainage tubing from the washport outlet to the waste container.

5. Prime or use the auto clean function to fill the wash solvent tubings.

6. Verify the autosampler setup within the OpenLAB Chemstation software.

Refer to Figure 22 for a diagram of standard capillary connections, showing connections from the solvent bottle, to the washport, sample loop flex, pump, column, waste, and needle seat, interacting with the peristaltic pump, metering device, and injection valve.

Preparations:

• Ensure the module is installed in the system.

• Use an appropriate wash solvent based on your sample and mobile phase chemistries. The wash solvent should be the most solubilizing compatible solvent (strongest diluent). Selecting the right wash solvent is part of method development.

• A mixture of 50% up to 100% organic solvent in distilled water is often a good choice.

NOTE: The standard silicone waste drainage tubing is suitable for most common wash solvents. For critical wash solvents, consider replacing it with a PE tube (Tubing, PE, 1.5 m, 5042-9974).

Steps:

1. Place a needle wash solvent reservoir into the solvent cabinet.

2. Connect the Needle Wash Bottle Head Assembly to the solvent reservoir and close the bottle.

3. Guide the tube from the Needle Wash Bottle Head Assembly through the cover opening and connect it to the peristaltic pump.

4. Route the drainage tubing from the washport outlet to the waste container.

5. Prime or use the auto clean function to fill the wash solvent tubings.

6. Verify the autosampler setup within the OpenLAB Chemstation software.

Refer to Figure 23 for a diagram of dual-needle capillary connections, showing connections from the solvent bottle, to the washport, from the pump, sample loops (right and left needle), to the column, and needle seats (left and right), interacting with the peristaltic pump, peripheral valve, metering device, and injection valve.

The bypass capillary should be installed when the dual-needle configuration needs to be used in single-path mode. This setup allows for a minimized injection path, which helps reduce flush times. When the bypass capillary is installed on one needle’s flow path, that needle is no longer available for injection.

Tools required:

• p/n 8710-0510: Open-end wrench 1/4 – 5/16 inch

Parts required:

• p/n 5500-1238: Capillary ST 0.12 mm x 105 mm SL/SL

Preparations:

• Complete any ongoing acquisition jobs.

• Return any plate from the workspace back to the hotel.

• Remove the Sample Loop-Flex capillary that the bypass capillary will replace.

• Store the unused sample loop safely.

WARNING: Risk of injury by uncovered needle

An uncovered needle poses a risk of harm to the operator.

✓ Do not open the safety lock of the needle assembly.

✓ Be careful when working at the z-robot.

✓ Wear safety goggles when removing the needle assembly.

CAUTION: Mismatching sample loop configuration

Incorrect configuration can damage the system.

✓ Ensure that the sample loop configuration in the software matches the hardware installed.

NOTE: If you have installed or changed the bypass capillary, verify that the correct sample loop and bypass capillary settings are configured in the Chromatography Data System (CDS). Refer to “Setting up the Autosampler with Agilent OpenLab CDS ChemStation Edition”.

NOTE: For detailed instructions on setting up the dual-needle system, see “Modify Capillaries”.

1. Install the bypass capillary (p/n 5500-1238) onto either the left or the right flow path of the dual-needle system’s injection valve.

• For the left flow path, connect the bypass capillary from port 1 to port 5.

• For the right flow path, connect the bypass capillary from port 3 to port 7.

NOTE: Figure 24 illustrates the right bypass capillary installed, connecting port 3 to port 7.

2. To configure the bypass capillary in the Agilent OpenLab CDS ChemStation Edition:

• Right-click within the active area of the multisampler module display.

• Select Modify > Capillaries from the context menu that appears.

3. In the “Capillary Setup” dialog box:

• Locate the “Bypass capillary” dropdown menu.

• Select either Right or Left, corresponding to the flow path where you physically installed the bypass capillary.

4. Click Assign.

After assigning, you will observe that the syringe icon corresponding to the bypassed flow path is greyed out in the active area, indicating that only one flow path is now active for injection.

Preparations:

• Ensure the module is installed in the system.

• Use appropriate wash solvents based on your sample and mobile phase chemistries. Typically, one solvent is for needle wash and another for needle seat flushing. The composition should be the most solubilizing compatible solvent (strongest diluent). Selecting wash solvents is part of method development.

• A mixture of 50% up to 100% organic solvent in distilled water is often a good choice.

NOTE: The standard silicone waste drainage tubing is suitable for most common wash solvents. For critical wash solvents, consider replacing it with a PE tube (Tubing, PE, 1.5 m, 5042-9974).

Steps:

1. Place solvent reservoirs for both needle wash and needle seat flushing into the solvent cabinet.

2. Connect the Wash Bottle Head Assemblies to their respective solvent reservoirs and close the bottles.

3. Guide the tubes from the Wash Bottle Head Assemblies through the cover opening and connect them to the appropriate ports (S1, S2, S3) of the solvent selection valve (SSV).

4. Route the drainage tubing from the washport outlet to the waste container.

5. Prime or use the auto clean function to fill the wash solvent tubings.

6. Verify the autosampler setup within the OpenLAB Chemstation software.

Refer to Figure 25 for a diagram of multiwash capillary connections, showing connections from solvents on the cabinet, to the washport, flush pump, column, sample loop flex, washport/needle seat, metering device, and needle seat, interacting with the SSV/piezo pump, flush pump, metering device, and injection valve.

The setup for the Multisampler is displayed within the Agilent OpenLab CDS ChemStation Edition C.01.06 (and similar controllers like Local Controller, EZChrom Edition, MassHunter, though screens may vary).

NOTE: This information covers only the autosampler settings. For details on the ChemStation software itself or other InfinityLab LC modules, consult their respective documentation.

After successfully loading the OpenLab CDS ChemStation Edition, the Multisampler module should appear as an active item in the graphical user interface (GUI), typically shown in the “Method and Run Control” view (as depicted in Figure 26). This view provides an overview and control points for the entire LC system, including the Multisampler, pump, column compartment, valve, and detector.

The Multisampler user interface in ChemStation Edition features several active areas and displays key information:

Active Icons/Areas:

1. Power Icon: Click to turn the autosampler on or off.

2. Tray/Hotel Icon (+): Click to configure the sample hotel (assign wellplates).

3. EMF Button: Click to get the status of the EMF (Early Maintenance Feature).

4. Temperature Display (if cooler/thermostat installed): Shows current temperature; may be clickable for settings.

Displayed Information:

• Current Injection Volume: Shows the volume set for the next injection (e.g., 5.00 µl).

• Current Sample Location: Shows the vial or well location for the next injection (e.g., P1-A-7).

Moving the mouse cursor over these active icons (like the tray or EMF button) will typically change the cursor, indicating they are clickable for further actions or configuration.

Right-clicking within the active area of the Multisampler module in the ChemStation GUI opens a context menu with the following options:

• Control…: Opens the special module settings User Interface.

• Method…: Opens the Method User Interface (same as navigating via menu: Instrument > Set up Instrument Method > Setup G7167B).

• Injector Program…: Allows setting up a pretreatment/injector program, which replaces the standard injection cycle when activated.

• Identify Device: Initiates a device identification process.

• Home All: Sends all movable parts to their home positions.

• Switch on Tray Illumination: Toggles the sample area light.

• Auto-clean…: Opens settings for the automated cleaning procedure.

• Prime…: Opens settings for priming the sampler.

• Modify:

• Drawer Configuration: Allows changing the load capacity configuration of the Sample Hotel.

• Capillaries: Opens configuration for Sample Loop, Needle Seat, and bypass capillary.

• Reference Vial Rack: Configures the reference vial rack.

• Assign Wellplates: Opens the Wellplate Configuration (same action as clicking the Tray icon).

The Module Status indicator next to the module name (e.g., “Multisampler”) displays the current state using color coding and text:

• Error (Red): Indicates an error condition. “Error text” provides details.

• Not ready (yellow): Module is not ready for injection. “Not Ready text” provides the reason.

• Ready (green): Module is ready for the next run/injection.

• Pre run, Post run (purple): Indicates the module is in a pre-run or post-run state.

• Run (blue): Module is currently executing a run/injection.

• Idle (green): Module is powered on and ready but not actively running a sequence or method.

• Offline (dark gray): Module is not communicating with the software or is powered off.

• Standby (light gray): Module is in a low-power or standby state.

NOTE: For customizing wellplates, use the “Define Sample Containers” option in the main instrument configuration view, not through the right-click menu’s “Assign Wellplates”.

The EMF (Early Maintenance Feature) status indicator, usually represented by the “EMF” button, provides information about the maintenance status using color coding:

• Offline (gray): EMF feature status is not available or module is offline.

• Ok (Green): No Maintenance required.

• Warning (yellow): EMF warning. Maintenance might be required soon.

• Maintenance Required (red): EMF warning indicates maintenance is required.

The control settings for the multisampler are accessed by right-clicking on the active area of the graphical user interface for the module within the ChemStation software and selecting “Control…”.

NOTE: The configuration specific to the multisampler (like installed hardware options) is typically done through the module dashboard’s context menu (right-click options like Modify), not within the main instrument configuration window.

The Sampler control parameters, accessed via the “Control…” menu, include the following sections:

• Missing Vial:

• Ignore missing vial: Check this box to have the injector ignore a missing vial, log a message (“Missing vial “), and proceed with a 6-second dummy run before continuing to the next injection. If unchecked, a missing vial will likely cause an error.

• Illumination:

• Toggles the sample area illumination On or Off.

• At Power On (Available if cooler/thermostat installed):

• Turn on Thermostat: Check this box to automatically switch on the cooler/thermostat when the instrument is powered on.

• Thermostat (Available if cooler/thermostat installed & Constant temp mode selected):

• Select On to activate the cooler/thermostat and specify the target temperature. (Note: Temperature must be at least 5 °C below ambient for proper control).

• Select Off to deactivate the cooler/thermostat.

• Automatic Turn On (Available if cooler/thermostat installed):

• Allows setting a specific date and time for the cooler/thermostat to switch on automatically.

• Pump connected to Sampler:

• Specifies which pump (if multiple are configured) is used with the Vialsampler/Multisampler. Select the appropriate pump from the drop-down list.

• Clear Workspace:

• Immediately: Returns the sample container to the hotel immediately after injection completion (allows quick retrieval).

• At End of Analysis: Returns the sample container after the current run or sequence/worklist is finished (default setting).

• Never: Leaves the sample container on the workspace until a different container is needed.

• Enable Analysis (Requires LC & CE Drivers A.02.19+ and Sample Thermostat):

• With any temperature: Analysis can start regardless of the sample temperature.

• Temperature within +/- 2 degrees Celsius: Analysis will only start when the sample temperature is within ± 2 °C of the setpoint temperature.

NOTE: This section is disabled if the Thermostat is set to “Not controlled”.

Method Parameter Settings for the Multisampler are accessed either through the main menu via Instrument > Set up Instrument Method > Multisampler or by right-clicking on the Multisampler’s active area in the GUI and selecting Method….

These settings define the injection parameters for the method and typically include sections for:

• Injection Volume

• Needle Wash (Standard Wash, Multi-wash, Flush Port options)

• Stoptime / Posttime

• Advanced Settings (Sampling Speed, Needle Height Position, High Throughput options like Sample Flush-Out Factor, Bypass for Delay Volume Reduction, Overlapped Injection)

• Injection Cleaning Path

For additional help on specific fields, highlight the desired area in the settings dialog and press the F1 key to open context-sensitive help.

• Injection Volume: Set the desired volume for injection. The available range depends on the installed hardware configuration (e.g., default is 0.1 – 20 µL).

• Needle Wash: This section controls how the needle is cleaned.

• You can choose between Standard Wash or disabling it (Standard Wash off). Using a needle wash helps minimize carry-over.

• The Injection Cleaning Path section (often under an “Advanced” tab) provides further options:

▪ Standard Wash Mode: When Standard Wash is selected, you can choose between two modes:

– Flush Port: Uses the dedicated flush port for washing.

– Wash Vial: Uses a designated wash vial (if configured).

▪ Multi-wash Mode: If the Multi-Wash hardware option is installed, this mode becomes available, allowing more complex cleaning sequences using multiple solvents and steps (detailed separately).

NOTE: Usually, the default draw offset for needle height is 0, which corresponds to 2 mm above the wellplate bottom.

NOTE: For help on specific settings, highlight the cell and press F1.

The Multi-wash feature, available if the corresponding hardware is installed, allows for customized, multi-step cleaning procedures within the Injection Cleaning Path settings to minimize carry-over.

• Configuration: A table allows defining up to four cleaning steps, plus a “Start Cond.” step.

• Steps: For each step (1-4):

• Solvent: Select the solvent to use (S1, S2, S3) or turn the step Off using the dropdown arrow.

• Time [s]: Specify the duration for the step in seconds.

• Seat Back Flush: Check this box to include flushing of the needle seat during this step.

• Needle Wash: Check this box to include washing of the needle exterior during this step.

• Comment: Add an optional comment for the step.

• If both Seat Back Flush and Needle Wash are selected for a step, they occur simultaneously. If neither is selected, the step is ignored (same as selecting Off for the solvent).

• Start Cond. Step: This step (using solvent S1, S2, or S3) is intended to ensure the flow path is filled with the desired starting solvent for the next sample. It is recommended to check the box for this step, as it’s not always executed automatically.

• Stoptime: This setting determines the total duration of the analysis run. You can set a specific time in minutes or select “As Pump/No Limit” to let the pump’s program control the run time.

• Posttime: This setting defines a period after the Stoptime during which the system can perform actions like column equilibration before the next run starts. It can be set to a specific duration in minutes or turned “Off”.

It takes approximately 30 seconds to fully exchange one solvent for another in the flushport. Flushing and exchanging solvent in the needle seat takes about 18 seconds.

It is strongly recommended to use the Auto-Clean function regularly to flush the module with all installed wash solvents. This helps maintain cleanliness and prevent issues related to solvent incompatibility or residue buildup.

The Module Configuration settings for the Multisampler are accessed via the main menu: Instrument > Instrument Configuration > Multisampler Configuration.

NOTE: Changes to the sampler’s hardware configuration (e.g., changing loops, seats, installing options) can only be done in the online view of the CDS system, typically through the right-click context menu (Modify options) on the module’s dashboard, not through this Instrument Configuration window which primarily displays the currently recognized setup.

The Module Configuration view (accessed via Instrument > Instrument Configuration) displays the hardware and firmware details recognized by the system. For a single needle setup, it typically shows:

• Communication:

• Device name (e.g., Multisampler)

• Type ID (Product number, e.g., G7167B)

• Serial number

• Firmware revision

• Connection settings…

• Options / Drawer: Lists installed options recognized by the system.

• Metering: Shows the installed metering device (e.g., G4267-60042: 40 µL Analytical Head).

• Needle Information (Typically Right Needle for Single Setup):

• Loop: Displays the installed sample loop (e.g., G4267-60300: Sample Loop-Flex 20 µL).

• Seat: Displays the installed needle seat assembly (e.g., G4267-87012: Seat assembly 0.12 mm 129).

• Max. Injection Volume: Shows the maximum volume based on the installed loop and metering device (e.g., 20 µL). It may also indicate if Multi-draw is disabled.

• Installed Options Checkboxes:

• Cooler installed: Indicates if a sample cooler/thermostat is present.

• Mode: Shows the selected temperature control mode (if cooler installed).

• Multi-wash installed: Indicates if the multi-wash hardware is present.

• Reference vial rack: Displays the type of reference vial rack installed (e.g., G4267-40071: Reference vial rack (5)).

• Bypass capillary: Shows if a bypass capillary is installed (Typically “None” for standard single needle operation).

• Define Sample Containers…: Button to access wellplate configuration.

When working with a Dual Needle System, right-clicking into the Active Area of the Multisampler module in ChemStation provides a context menu with options including:

• Control…: Opens special module settings.

• Method…: Opens the Method setup interface (same as Instrument > Set up Instrument Method > Setup G7167B).

• Injector Program…: Configures pretreatment/injector programs.

• Identify Device: Identifies the connected module.

• Home All: Moves all axes to their home positions.

• Reset Injector: Resets the injector mechanism.

• Switch to Mainpass Left: Configures the system to use the left needle loop connected to the pump device as the main injection path.

• Switch to Mainpass Right: Configures the system to use the right needle loop connected to the pump device as the main injection path.

• Switch on Tray Illumination: Toggles the sample compartment light.

• Auto-clean…: Opens the automated cleaning procedure settings.

• Prime Solvents…: Opens solvent priming settings.

• Start Purge: Manually initiates the system purge routine.

• Modify: (Submenu for changing hardware configuration like Drawers, Capillaries, Reference Vial Bar)

• Assign Wellplates: Opens the wellplate configuration interface.

Accessed via the right-click menu for the Dual Needle system:

• Auto-clean…: Opens the “Auto-clean Settings and Start” dialog. This allows configuring and initiating an automated cleaning routine. Options typically include:

• Switching the Injection Valve.

• Washing the Right Needle and/or Left Needle for a specified Duration. Each selected needle is purged during this time.

• Option to Purge the Sampler (requires setting pump composition accordingly beforehand).

• Prime Solvents…: Opens the “Prime Solvents Settings” dialog. This allows priming the wash solvent lines. Options include:

• Setting the Duration for priming (e.g., 5 seconds).

• Note: The Multisampler primes the wash port using the first configured solvent (S1).

The Start Purge option, available in the right-click context menu for the Dual Needle system, manually initiates the purge routine. The duration of this purge is determined by the hydraulic setup of the system (e.g., pump type, flow rate, installed volumes). See the “Purge” details for typical times and further information.

The Modify submenu, accessed via the right-click context menu, allows configuration of the installed hardware:

• Drawer Configuration: Changes the loading capacity setup of the Sample Hotel (e.g., number and type of drawers).

• Capillaries Setup: Configures the installed sample loops, needle seats, and bypass capillaries specific to the dual needle option.

• Reference Vial Bar: Configures the type of reference vial bar installed.

Custom wellplates are defined using the Assign Wellplates option from the right-click menu (or by clicking the Tray icon) which opens the Wellplate Configuration interface.

NOTE: For more advanced customization or defining new plate types not already in the system’s library, you typically need to use the Define Sample Containers option found within the main Instrument Configuration view (Instrument > Instrument Configuration > Multisampler Configuration).

Typical purge times depend on the pump and flow rate:

• 1290 Binary pump, 2x 20 µL setup, flow rate: 0.5 mL/min ≈ 125 seconds

• 1290 Binary pump, 2x 20 µL setup, flow rate: 1.0 mL/min ≈ 85 seconds

Other configurations, especially those with larger volumes, will take longer.

To monitor the remaining purge time, expand the Multisampler module window in the user interface. The “Purge Status” line will display the remaining time in seconds (e.g., “107 s”). The interface will also indicate the “Not ready condition” (e.g., “Cleaning”) and if a bypass capillary is installed.

The purge routine is automatically triggered by the start of the pump or by changes in the solvent composition being delivered by the pump.

The purpose of the purge routine is to flush the entire hydraulic setup of the multisampler (including the metering device, sample loops, and needles) with fresh mobile phase. This ensures the flowpath is clean and conditioned with the correct mobile phase before an injection or sequence begins.

The only way to significantly speed up the purge routine is to increase the flow rate from the pump during the purge.

Best practice involves creating a dedicated “purge method” within the sequence table. This method should set a high flow rate. Ideally, it should also include control of a column switching valve (if available) to direct the high flow rate to waste, bypassing the column during the purge, before switching back for the analytical run.

NOTE: For pumps with a manual purge valve, it is mandatory to manually start the purge routine before beginning a run or sequence to ensure the complete dual needle flow path is flushed with fresh mobile phase.

Capillary configuration for the Dual Needle system is accessed by right-clicking the Multisampler module in the ChemStation GUI and selecting Modify > Capillaries. This opens the “Capillary Setup” dialog box (shown in Figure 30).

In this dialog, you can view and potentially select (if configuring offline or if options allow) the installed components for both the Left and Right flow paths:

• Loop Capillary Left/Right: Select the appropriate sample loop installed for each path (e.g., G4267-60301: Loop 20 µL left Dual-Needle).

• Seat Capillary Left/Right: Select the appropriate needle seat assembly installed for each path (e.g., G4267-87012: Seat assembly 0.12 mm 1290 Infini).

• Bypass capillary: Select whether a bypass capillary is installed on the Left, Right, or None.

The dialog also displays associated physical parameters like Physical Volume, Injection Volume limits, Inner Diameter, and Length for the selected components.

• Match Hardware: To avoid system damage, the configuration selected in the software (especially the sample loops) MUST accurately match the physically installed hardware.

• Bypass Capillary Type: If using a bypass capillary (either left or right), only the specified Agilent capillary PN 5500-1238 (Capillary ST 0.12 mm x 105 mm SL/SL) should be used.

• Sample Loops Type: For the dual needle setup, use only the correct dual needle sample loops, such as Sample Loop 20 µL right Dual needle (G4267-60311). These loops are specifically manufactured for dual-needle systems.

The Instrument Configuration View (accessed via Instrument > Instrument Configuration) for a dual needle setup configured for alternating use with identical sample loops (e.g., two 20 µL loops) will display the details for both the Left and Right needles. Key indicators include:

• “Left needle installed” checkbox is ticked.

• “Alternating needle usage possible” is indicated (enabled because left and right hardware configurations match).

• Details for both “Left needle” and “Right needle” sections show the identical Loop (e.g., G4267-60301 and G4267-60311, both 20 µL Dual-Needle types) and Seat assemblies (e.g., G4267-87012).

• The Max. Injection Volume will be the same for both (e.g., 20 µL).

Figure 31 provides a visual example of this configuration screen.

The Instrument Configuration View for a dual needle setup with non-identical flow paths (e.g., a 20 µL loop on the left and a 500 µL loop on the right) will display:

• “Left needle installed” checkbox is ticked.

• “Alternating needle usage not available” is indicated (because the left and right configurations differ).

• Details under “Left needle” show the components for that path (e.g., Loop G4267-60301: 20 µL, Seat G4267-87012).

• Details under “Right needle” show the different components for that path (e.g., Loop G7167-68511: 500 µL, Seat G4267-87012).

• The Max. Injection Volume will differ between the left and right needles (e.g., 20 µL and 500 µL respectively).

Figure 32 provides a visual example of this configuration.

NOTE: In this view, it is not possible to change the online configuration of the sample loops or seat capillaries. It only shows the currently installed and detected devices and their status.

The Instrument Configuration View provides the following details:

• Communication:

• Device name (e.g., Multisampler)

• Type ID (e.g., G7167A/B)

• Serial number (e.g., DEBAR00101)

• Firmware revision (e.g., D.06.75)

• Connection settings (e.g., LAN connection or hostname)

• Options: Lists installed hardware options.

• Metering: Specifies the installed metering device. NOTE: For dual needle, only the 100 µL metering device (G4267-60043) is available. Single needle can use 40 µL, 100 µL, or 900 µL devices.

• Left Needle installed: Check box indicating if the dual-needle option is equipped. If checked, the Left Needle section is enabled. NOTE: If Left and Right Needle parameters are equivalent, Alternating Needle Usage is possible, increasing sampling efficiency. NOTE: With dual-needle installed, multi-load (not multi-draw) is used for larger volumes. Multi-draw is unavailable with multi-wash and dual-needle.

• Left Needle: (Enabled if ‘Left Needle installed’ is checked)

• Loop: Shows the currently installed loop capillary for the left path. NOTE: Configuration must match installed hardware to avoid damage.

• Seat: Shows the currently installed seat capillary for the left path.

• Right Needle: (Always enabled)

• Loop: Shows the currently installed loop capillary for the right path.

• Seat: Shows the currently installed seat capillary for the right path.

Continuing from Part 1, the Instrument Configuration View also details:

• Loop (Left/Right): Shows the currently installed loop capillary. NOTE: For dual needle, only correct dual needle sample loops must be configured and used (e.g., G4267-60311, Sample Loop 20 µL right Dual needle). These are specifically made for dual-needle systems.

• Seat (Left/Right): Shows the currently installed seat capillary. Its volume is used for Automatic Delay Volume Reduction (ADVR) calculations and ISET (Intelligent System Emulation Technology).

• Thermostat installed: Check box marked if a sample cooler or thermostat is installed.

• Mode (if Thermostat installed):

• Constant temperature mode: Set temperature via Multisampler Control parameters. Recommended for constant temperature across runs.

• Variable temperature mode: Set temperature via Advanced Method Setup parameters, allowing temperature changes between runs.

• Multi-wash installed: Check box marked if the multi-wash option is installed. If installed, the Multi-wash option appears in the Needle Wash section of Method parameters. (Note: Multi-wash is not available for dual-needle operation).

• Reference vial rack: Drop-down list to select the type of reference vial rack installed in the multisampler.

Concluding the details from the Instrument Configuration View:

• Bypass capillary: Drop-down selection (None, Left, Right) indicating if and where a bypass capillary is installed. When installed (Left or Right), it enables a minimized injection path for reduced flush times and allows using the dual-needle setup in single-path mode. The needle corresponding to the bypassed path is then unavailable for injection. NOTE: Only the listed capillary p/n 5500-1238 can be used as a bypass capillary (either left or right). NOTE: For dual needle setup, only the correct hardware must be configured and used, e.g., Capillary ST 0.12 mm x 105 mm SL/SL (5500-1238) or Sample Loop 20 µL right Dual needle (G4267-60311).

• Define Sample Containers…: Button that opens the “Define and edit Wellplates configuration” dialog box. This allows managing the list of standard preconfigured wellplates and any custom wellplates that have been added.

The Method Setup screen for a Multisampler with the Dual Needle option installed (accessed via Instrument > Set up Instrument Method or right-click > Method…) includes specific fields related to dual-needle operation. Key areas shown in Figure 33 include:

• Injection Section:

• Injection volume

• Needle selection (Dropdown: Alternating Needle, Right Needle, Left Needle)

• Needle Wash Section: (Standard Wash options)

• Stoptime / Posttime Section:

• Advanced Section:

• Sampling Speed (Draw, Eject, Wait Time After Draw)

• Needle Height Position (Offset, Use Vial/Well Bottom Sensing checkbox)

• Smart Overlap Section: (Available for alternating needle)

• Enable Smart Overlap checkbox

• After Period of Time setting

• Injection Path Cleaning Button: Accesses detailed wash settings.

Key Method Setup parameters for Dual Needle (DN) include:

• Injection: Specifies the Injection volume.

• Needle selection:

• Alternating Needle: Needles are toggled for injection (only possible if both flow paths are configured identically).

• Right Needle: Only the right needle is used.

• Left Needle: Only the left needle is used.

• Needle Wash: Governed by settings in the Standard Wash section of the Injection Path Cleaning parameters.

• Stoptime: Sets the end time for the analysis run. Limits: 0.01 to 99999 min or As Pump/No Limit.

• Posttime: Defines a period after the Stoptime for equilibration or other post-run activities before the next analysis starts. Limits: 0.01 to 99999 min or Off (0.0 min).

• Sampling Speed:

• Draw Speed: Rate at which the plunger draws sample. Use a slower speed for viscous samples.

• Eject Speed: Rate at which the plunger ejects sample from the metering device. Higher speeds shorten injection cycle time for large volumes. Use a slower speed for viscous samples.

• Wait Time After Draw: Ensures temporary vacuum from drawing liquid dissipates. The needle stays on seat, then remains in the vial for this specified time after drawing.

Continuing the Method Setup parameters for Dual Needle (DN):

• Needle Height Position:

• Offset: Vertical offset (in mm) from the needle’s standard position. Useful for sampling specific layers (e.g., top layer) or very small volumes. (Default 0 offset ≈ 2 mm above wellplate bottom).

• Use Vial/Well Bottom Sensing: Checkbox to enable detection of non-uniform well bottoms. Adjusts needle depth to 2 mm (default) above the detected bottom. Can be combined with Offset. Default is off. Turn off to increase injection speed or if sample precipitate might clog the needle.

• Smart Overlap: (Available only for dual-needle with identical flow paths and Alternating Needle selected)

• Enable Smart Overlap: Checkbox to enable overlapped injection. Allows preparation of the next injection (on the alternate needle) while the current injection is running in the main path, increasing sample throughput.

• After Period of Time: Specifies the time (in minutes) the Multisampler waits after injecting one sample before picking up and injecting the next sample in overlapped mode.

NOTE: It is important to calculate this time accurately (close to the start point of the next run’s gradient or data acquisition) to avoid unnecessary waiting time with a filled sample loop.

The High Throughput settings section is available for a dual-needle Multisampler under specific conditions (e.g., non-identical capillaries, or Right/Left Needle selected). It contains parameters to optimize speed and reduce delay volume:

NOTE: This section is specifically applicable under conditions where Smart Overlap (for identical alternating needles) is not available, i.e., when using different seat/loop capillaries or when Right Needle or Left Needle mode is selected.

The Not ready timeout is a setting found in the Sequence Parameters (Sequence output tab). It defines the maximum time (in minutes) the system will wait for all modules to become ready before starting the next run in a sequence.

This setting is particularly important when automated purge routines are triggered (e.g., by pump start or solvent changes) at the beginning of a run. The purge routine takes a certain amount of time. The “Not ready timeout” value must be set longer than the duration of the purge routine. If the timeout value is shorter than the purge time, the system will abort the run before the purge completes and the instrument becomes ready, preventing the sequence from proceeding correctly.

• Delay volume: This is defined as the system volume between the point where solvents are mixed in the pump and the top (inlet) of the analytical column.

• Extra-column volume: This is defined as the volume between the point of injection (typically the injection valve) and the point of detection (detector flow cell), but it excludes the volume within the column itself.

In gradient separations, the delay volume causes a time lag between when the mobile phase composition changes at the pump and when that change actually reaches the column inlet. This effectively adds an initial isocratic segment to the start of every gradient run, the duration of which depends on the flow rate and the delay volume.

While gradient profiles are usually reported based on the pump settings, the delay volume itself isn’t typically quoted, yet it influences the actual separation. This effect is more pronounced at lower flow rates and with smaller column volumes. It can significantly impact the transferability of gradient methods between different LC systems. Therefore, for fast gradient separations, especially with narrow-bore columns (like 2.1 mm i.d.) often used in LC/MS, minimizing delay volume is important.

The delay volume contributed by the multisampler (flow path through metering device, needle, seat, capillaries back to injection valve – approx. 78 µL for G7167B or 265 µL for G7167A) can be effectively reduced without physical hardware changes by using the Automatic Delay Volume Reduction (ADVR) function.

Normally, after injection, the valve stays in the mainpass position, meaning the gradient must flow through the entire autosampler loop volume to reach the column. ADVR changes this behavior.

By selecting the ADVR function in the autosampler setup menu (often found under High Throughput settings as “Injection Valve to Bypass for Delay Volume Reduction”), the injection valve is switched from mainpass to bypass shortly after the injected sample has been flushed onto the column. The system ensures sufficient flush time based on the “Flush-out Factor” setting (typically 5 times the injection volume plus loop/seat/valve contributions).

Switching to bypass removes the autosampler’s internal loop volume from the flow path during the remainder of the gradient run. This significantly reduces the overall system delay volume (e.g., by approx. 50 µL for G7167B or 240 µL for G7167A with a 1 µL injection under standard conditions), which is beneficial for fast gradients (e.g., under 0.5 min).

The schematic injection steps for a single needle Multisampler involve switching the injection valve between mainpass and bypass positions:

1. Mainpass Flow (Figure 34): In the default state or during analysis, the injection valve is in the mainpass (position 1-6, 2-3, 4-5). Mobile phase flows from the pump (via port 1), through the sample loop (ports 6 to 5), out to the column (via port 4). The metering device and needle seat path (ports 2 and 3) are isolated or flushed by the wash pump.

2. Bypass – Drawing Sample (Figure 35): To draw sample, the valve switches to bypass (position 1-2, 3-4, 5-6). The pump flow now goes directly from port 1 to port 2, through the needle seat path (port 3), and out to the column (port 4). The sample loop (ports 6 and 5) is isolated from the main flow. The metering device draws the sample from the vial through the needle into the sample loop via port 6.

3. Bypass – Washing Needle (Figure 36): While still in bypass, the needle can be washed externally at the wash port. The flow path remains 1-2-3-4 to the column. The wash pump delivers solvent to the wash port.

4. Mainpass – Sample Injected (Figure 37): The valve switches back to mainpass (position 1-6, 2-3, 4-5). The pump flow now enters at port 1, pushes the drawn sample out of the sample loop (from port 6 through port 5), and carries it to the column via port 4. The needle seat path (ports 2 and 3) is again isolated or connected to the wash system.

When using ADVR, the gradient starts at the pump at the instant of injection. Consider if the gradient has reached the autosampler, which can cause a small step in the gradient results. This occurs if the delay volume is less than the flush-out volume. This might be a factor in method transfer.

With a flush-out factor of 5 and a 10 μL injection volume, the autosampler allows 50 µL to pass before switching to bypass. If the delay volume is 50 µL, the gradient just reaches the injection valve. Smaller injection volumes have no effect, but larger volumes introduce a small gradient step.

Yes, the flow rate impacts the decision. At 0.2 mL/min, the delay time saved is 21 seconds, while at 1.0 mL/min, it is 4 seconds.

ADVR is unlikely suitable for applications with compounds known to cause carry-over problems.

To reduce delay volume, install the 40 µL Analytical Head and the 20 μL Loop. For best results, use the Low Dispersion Heat Exchanger and the micro flow cell for UV. This reduces the delay volume by 60 μL or 250 μL.

The standard configuration can inject a maximum of 20 μL (G7167B) or 100 µL (G7167A) with standard loop capillaries.

To increase injection volume, a Multidraw Kit can be used. This involves installing an extension capillary between the needle seat and injection valve. Available kits are the Large Volume Injection Kit (G4216-69711, 80 µL extra volume) and the Multidraw Kit (G7167-68711, 400 or 1400 µL extra volume).

For higher injection volumes, install larger analytical heads and sample loops: 100 µL Analytical Head with 40 or 100 µL loops (for G7167B), and 900 µL Analytical Head with 500 or 900 µL loops (for G7167A and G7167B).

Double the volume of the extended capillary. The system delay volume increases accordingly.

Reduce the injection volume proportionally to the column volume to maintain performance. This keeps the injection volume at the same percentage volume relative to the column.

It’s particularly important if the injection solvent is stronger (more eluotropic) than the starting mobile phase. An increase affects separation, especially for early running peaks (low retention factor), potentially causing peak distortion.

Keep the injection solvent the same or weaker than the starting gradient composition.

Check for signs of increased dispersion, such as wider or more skewed peaks and reduced peak resolution.

If injection is made in a weak solvent, the volume can likely be increased as it concentrates the analyte on the column head at the start of the gradient.

Increased injection volume will spread the analyte band down the column ahead of the gradient, resulting in peak dispersion and loss of resolution.

The column diameter is a main consideration as it significantly impacts peak dispersion. Narrower columns can yield higher peaks with less dispersion compared to wider columns with larger injections.

Typical injection volumes might range from 5 to 10 µL, but it depends heavily on analyte chemistry and mobile phase.

Injection volumes of about 5% of the column volume might be achievable while maintaining good resolution and peak dispersion.

Use a trapping column selected by a switching valve to capture and concentrate the injection before switching it onto the analytical column. This valve can be located in the Multicolumn Thermostat.

Optimizing injection speed involves balancing speed and reproducibility. Drawing the sample too fast can reduce reproducibility. Marginal gains are typical as sample volumes are usually small.

Needle movements to and from the vial and into the flush port take a significant portion of injection time.

Overlapped injection performs needle manipulations (aspirating next sample, preparing for injection) while the previous separation is running. This can be turned on in the Multisampler setup software. The Multisampler switches flow to bypass after injection and starts preparing the next sample during the run (e.g., 3 minutes into a 4-minute run).

Overlapped injection can typically save 0.5 to 1 minute per injection.

Increased resolution improves qualitative and quantitative data analysis, allows more peaks to be separated, or offers scope for speeding up the separation.

Optimize selectivity

Smaller particle-size packing

Longer columns

Shallower gradients, faster flow

Rs = (1/4) * √N * ( (α – 1) / α ) * ( (k₂ + 1) / k₂ )

Rs = resolution

N = plate count (measure of column efficiency)

α = selectivity (between two peaks)

k₂ = retention factor of the second peak (formerly called capacity factor).

Selectivity (α) has the most significant effect on resolution.

Varying selectivity involves changing the stationary phase type (e.g., C18, C8, phenyl, nitrile), the mobile phase, and temperature to maximize differences between solutes.

It’s best done with an automated method development system, assessing a wide range of conditions on different columns and mobile phases using an ordered scouting protocol, often starting with short columns for fast analysis.

The plate count or efficiency (N) is the next most significant term.

N is inversely proportional to particle size and directly proportional to column length. Smaller particle size and longer columns result in higher plate numbers.

Pressure rises with the inverse square of the particle size and proportionally with column length. The 1290 Infinity II LC System is designed for up to 1300 bar to run sub-2-micron particles and longer columns (100 mm, 150 mm, or even linked columns up to 250 mm).

Resolution increases with the square root of N. Doubling the column length increases resolution by a factor of 1.4.

Achievable resolution depends on mobile phase viscosity as it relates directly to pressure. Methanol mixtures generate more backpressure than acetonitrile mixtures.

Acetonitrile is often preferred for better, narrower peak shapes and lower viscosity. Methanol generally yields better selectivity, especially for small molecules (<500 Da).

Increasing temperature reduces viscosity but can change separation selectivity. Experimentation is needed to see if selectivity increases or decreases.

Frictional heating inside the column increases with flow and pressure, potentially leading to slightly increased dispersion and small selectivity changes, both possibly reducing resolution. This might be offset by slightly reducing thermostat temperature.

The van Deemter curve shows optimum flow rate is higher and flatter for STM columns compared to larger particles. Typical near-optimum flow rates are 2 mL/min for 4.6 mm i.d. and 0.4 mL/min for 2.1 mm i.d. columns.

Increasing the retention factor (k) results in better resolution because the solute is retained longer.

Retention in gradient separations is described by k* (mean k value).

k* = (tG / Vm) * F * (100 / Δ%B) * (1 / S)

k* = mean k value

tG = time length of gradient (or segment) (min)

F = flow (mL/min)

Vm = column delay volume

Δ%B = change in fraction of solvent B during the gradient

S = constant (approx. 4–5 for small molecules)

Increase k* by using a shallower gradient (e.g., 2-5%/min change), a higher flow rate, and a smaller column volume.

Double the flow rate (F) and halve the gradient time (tG). k* remains constant, and the separation looks the same but occurs in half the time.

Yes, research shows shorter STM columns (especially at temperatures > 40 °C) can generate higher peak capacity than longer STM columns by running them faster.

Sensitivity is linked to the choice of stationary and mobile phases (for good separation, narrow peaks, stable baseline, minimal noise) and instrument configuration, especially detector setup.

Pump mixer volume

Narrower columns

Detector flow cell

Detector parameters

Selectivity and linearity are related topics.

Sensitivity is specified as signal-to-noise ratio (S/N). Maximize peak height and minimize baseline noise.

Minimize extra-column volume by using short, narrow internal diameter connection capillaries and correctly installed fittings.

Using smaller i.d. columns should result in higher peak height, ideal for limited sample amounts. Injecting the same sample amount on a smaller i.d. column reduces dilution due to column diameter, increasing sensitivity.

Decreasing column i.d. from 4.6 mm to 2.1 mm results in a theoretical peak height gain of 4.7 times due to decreased dilution.

The lower flow rates used with narrow columns can result in higher ionization efficiencies, leading to higher sensitivity in mass spectrometry.

Carryover is measured by residual peaks from a previous active injection appearing in a subsequent blank solvent injection. It’s reported as the peak area in the blank relative to the area in the previous active injection (as a percentage).

Optimized by careful flow path design and use of materials minimizing sample adsorption. A carryover figure of 0.001% should be achievable, even with a triple quadrupole MS detector.

Use appropriate operating settings and functions of the Multisampler.

Internal needle wash

External needle wash

Needle seat backflush

Injection valve cleaning

The flow path, including the inside of the needle, is continuously flushed during normal operation.

ADVR reduces both delay volume and flushing of the standard Multisampler, so it should not be used with analytes where carryover might be a problem.

Use a wash vial in a specific location or the flush port.

The wash vial should have no septum and contain a suitable solvent for washing the sample off the needle. No septum is used to avoid wiping contamination off on the way down and reapplying it on the upstroke. The needle can be dipped multiple times.

Using the flush port is more effective than using a wash vial.

Located above and behind the needle seat. In standard configuration, a peristaltic pump delivers wash solvent. It has a volume of 0.68 mL, and the pump delivers 5 mL/min, refilling the port completely in 7 seconds.

The duration can be set by the user. It can last 2-3 seconds for routine situations or 10-20 seconds for complete washing if carryover is a greater concern.

It’s recommended to avoid contaminating the needle seat.

It must be back-flushed.

In the standard setup, it’s done manually by changing flow connections. It can be automated using the Flexible Cube (G4227A). In the Multiwash setup (G4757A), it’s automated and can use up to three different solvents.

Regularly flush the port, solvent delivery pump, and tubing. For example, prime the flush pump for three minutes daily with appropriate solvent before use.

Analyte might be adsorbing to the inner surfaces of the injection valve. Activate the auto clean feature in the CDS; the valve will make additional switching movements to clean the flow path.

Switch the injection valve at the high organic percentage after the last peak elutes. Also, switch it again after initial mobile phase conditions have stabilized.

It ensures the bypass groove in the rotor seal contains the gradient start conditions, crucial for flow rates below 0.5 mL/min.

Use wash vials with an appropriate solvent if the outside of the needle cannot be cleaned sufficiently with water or alcohol from the flush pump. An injector program allows using several wash vials.

After a run-in period for new instruments or after exchanging consumable parts (needle, needle seat, valve parts), during which surfaces adjust.

Back-flush the needle seat to clean the sealing areas. Regular preventive maintenance is recommended as carry-over performance depends on the integrity of consumables.

Chapter 6 gives an overview of troubleshooting and diagnostic features and the different user interfaces.

Yes, available tests and screens/reports may vary depending on the user interface.

The preferred tool should be Agilent Lab Advisor Software.

No, Agilent OpenLAB ChemStation C.01.03 and above do not include maintenance/test functions.

Screenshots are based on the Agilent Lab Advisor Software.

It’s a standalone product (basic license shipped with an Agilent LC pump) usable with or without a CDS. It helps manage the lab for high-quality results by providing a system overview of connected instruments, status, EMF counters, configuration info, and diagnostic tests.

A detailed diagnostic report can be generated, which can be sent to Agilent for improved troubleshooting and repair.

Lab Advisor Basic and Lab Advisor Advanced.

Lab Advisor Basic is included with every instrument.

Advanced features (unlocked by license key) include real-time monitoring of instrument actuals, signals, state machines. Diagnostic/calibration results and signal data can be uploaded to a shared folder. The Review Client allows examining uploaded data from any instrument.

It’s ideal for internal support groups and users tracking instrument history.

It provides an easy-to-use, step-by-step multimedia guide for preventive maintenance on Agilent 1200 Infinity and InfinityLab LC Series instruments.

Yes, they may differ. Refer to the Agilent Lab Advisor software help files for details.

Chapter 7 describes the meaning of error messages and provides information on probable causes and suggested actions to recover from error conditions.

Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs that requires attention before analysis can continue (e.g., repair or consumable exchange).

The red status indicator at the front of the module switches on, and an entry is written into the module logbook.

Other modules will not be informed about the error.

All connected modules get a notification, all LEDs turn red, and the run will be stopped. The stop implementation differs by module type (e.g., pump flow stops for safety; detector lamp stays on to avoid equilibration time).

Depending on the error type, the next run can only be started if the error has been resolved (e.g., leak dried). Errors from presumably single events can be recovered by switching the system on in the user interface.

Leaks are potential safety issues. A leak always causes a shutdown of all modules, even outside a method run, as it might have originated from a different module than where it was detected.

Error propagation is done via the CAN bus or via an APG/ERI remote cable.

General error messages are generic to all Agilent series HPLC modules and may appear on other modules as well.

The timeout threshold was exceeded.

Probable Cause 1: The analysis completed successfully, and the timeout function switched off the module as requested.

Suggested Action 1: Typically, no action is required if analysis completed successfully. Check the logbook if necessary.

Probable Cause 2: A not-ready condition was present during a sequence or multiple-injection run for longer than the timeout threshold.

Suggested Action 2: Check the logbook for the occurrence and source of the not-ready condition. Restart the analysis where required.

An external instrument generated a shutdown signal on the remote line. A LOW signal on pin 4 of the remote connector triggers this.

Probable Cause 1: Leak detected in another module with a CAN connection.

Suggested Action 1: Fix the leak in the external instrument before restarting the module.

Probable Cause 2: Leak detected in an external instrument with a remote connection.

Suggested Action 2: Fix the leak in the external instrument before restarting the module.

Probable Cause 3: Shut-down occurred in an external instrument with a remote connection.

Suggested Action 3: Check external instruments for a shut-down condition.

Probable Cause 4: The degasser failed to generate sufficient vacuum for solvent degassing.

Suggested Action 4: Check the vacuum degasser for an error condition. Refer to the Service Manual for the degasser or the pump with the built-in degasser.

A not-ready condition is still present on the remote input. The system expects not-ready conditions to switch to run conditions within one minute of starting analysis. If still present after one minute, this error occurs.

Probable Cause 1: Not-ready condition in one of the instruments connected to the remote line.

Suggested Action 1: Ensure the instrument showing not-ready is installed correctly and set up correctly for analysis.

Probable Cause 2: Defective remote cable.

Suggested Action 2: Exchange the remote cable.

Probable Cause 3: Defective components in the instrument showing the not-ready condition.

Suggested Action 3: Check the instrument for defects (refer to its documentation).

During analysis, internal synchronization or communication between modules failed. One or more modules are no longer recognized as connected.

Probable Cause 1: CAN cable disconnected.

Suggested Action 1: Ensure all CAN cables are connected correctly. Ensure all CAN cables are installed correctly.

Probable Cause 2: Defective CAN cable.

Suggested Action 2: Exchange the CAN cable.

Probable Cause 3: Defective mainboard in another module.

Suggested Action 3: Switch off the system. Restart the system and determine which module(s) are not recognized.

The leak sensor has failed (short circuit). Leak sensor current changes when cooled by solvent; if the current increases above the upper limit, this error occurs.

Probable Cause 1: Defective leak sensor.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Leak sensor incorrectly routed, pinched by metal.

Suggested Action 2: Please contact your Agilent service representative.

Probable Cause 3: Power switch assembly defective.

Suggested Action 3: Please contact your Agilent service representative.

Probable Cause 4: Cable or contact problem.

Suggested Action 4: Please contact your Agilent service representative.

The leak sensor has failed (open circuit). Leak sensor current changes when cooled by solvent; if the current falls outside the lower limit, this error occurs.

Probable Cause 1: Leak sensor not connected to the Power Switch board.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Defective leak sensor.

Suggested Action 2: Please contact your Agilent service representative.

Probable Cause 3: Leak sensor incorrectly routed, pinched by metal.

Suggested Action 3: Please contact your Agilent service representative.

Probable Cause 4: Power switch assembly defective.

Suggested Action 4: Please contact your Agilent service representative.

The ambient-compensation sensor (NTC) on the power switch board has failed (open circuit). This sensor compensates for ambient temperature changes in the leak detection circuit. Error occurs if resistance increases above the upper limit.

Probable Cause 1: Loose connection between the power switch board and the mainboard.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Defective power switch assembly.

Suggested Action 2: Please contact your Agilent service representative.

The ambient-compensation sensor (NTC) on the power switch board has failed (short circuit). This sensor compensates for ambient temperature changes in the leak detection circuit. Error occurs if resistance falls below the lower limit.

Probable Cause 1: Defective power switch assembly.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Loose connection between the power switch board and the mainboard.

Suggested Action 2: Please contact your Agilent service representative.

The fan in the autosampler module or Sample Cooler/Thermostat has failed. The main board monitors fan speed via a hall sensor; error occurs if speed drops below 2 revolutions/second for longer than 5 seconds.

Depending on the module, assemblies (like the detector lamp) are turned off to prevent overheating.

Probable Cause 1: Fan cable disconnected.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Defective fan.

Suggested Action 2: Please contact your Agilent service representative.

Probable Cause 3: Defective Sample Cooler/Sample Thermostat fan.

Suggested Action 3: Replace the Sample Cooler/Sample Thermostat.

Probable Cause 4: Defective main board.

Suggested Action 4: Please contact your Agilent service representative.

A leak was detected in the module. The leak algorithm uses signals from the leak sensor and the board-mounted temperature-compensation sensor. A leak cools the leak sensor, changing its resistance, which is sensed by the circuit.

Probable Cause 1: Loose fittings.

Suggested Action 1: Ensure all fittings are tight.

Probable Cause 2: Broken capillary.

Suggested Action 2: Exchange defective capillaries.

Probable Cause 3: Leaking rotor seal or needle seat.

Suggested Action 3: Exchange the rotor seal or seat capillary.

Probable Cause 4: Defective metering seal.

Suggested Action 4: Exchange the metering seal. Make sure the leak sensor is thoroughly dry before restarting the autosampler.

Probable Cause 5: Leaking peristaltic pump.

Suggested Action 5: Exchange the peristaltic pump.

Please verify the first errors in the list. The last error message could be a subsequent error.

The robot (sample handler) failed to move correctly during the injection sequence.

Probable Cause 1: Missing vessel.

Suggested Action 1: Check if the sample vial is installed in the correct position, or edit the method or sequence accordingly.

Probable Cause 2: Needle command failed.

Suggested Action 2: Check the status of the needle assembly. Perform an autoreferencing.

No vial was found in the position defined in the method or sequence. When the needle moves to a vial and lowers, an encoder monitors the needle position. If no vial is present, the encoder detects an error.

Probable Cause 1: No vial in the position defined in the method.

Suggested Action 1: Install the sample vial in the correct position. Edit the method or sequence accordingly.

Probable Cause 2: Defective needle assembly.

Suggested Action 2: Exchange the needle assembly.

Probable Cause 3: Sample container missing or not correctly installed.

Suggested Action 3: Install the sample container correctly on the tray.

The autosampler failed to complete initialization correctly. During initialization, the robot moves to reference positions, and the processor monitors sensors and encoders. It also checks the status of the sample hotel and hydraulic box. If movements or status checks fail, the error occurs.

Probable Cause 1: Front door not installed correctly.

Suggested Action 1: Check if the front door is installed correctly. Check if the magnet is in place in the front door.

Probable Cause 2: Sample handler not aligned correctly.

Suggested Action 2: Do an autoreferencing.

Probable Cause 3: Mechanical obstruction of the sample handler.

Suggested Action 3: Ensure unobstructed movement.

Probable Cause 4: Defective sample handler motors.

Suggested Action 4: Please contact your Agilent service representative.

Probable Cause 5: Loose connection between hydraulic box and adapter board.

Suggested Action 5: Please contact your Agilent service representative.

Probable Cause 6: Defective sample hotel electronic.

Suggested Action 6: Please contact your Agilent service representative.

Probable Cause 7: Defective specific main board or fusion board.

Suggested Action 7: Please contact your Agilent service representative.

The autosampler failed to initialize the injection valve correctly. During initialization, the processor monitors sensors and the actuator motor for the valve. If movements or status checks fail, the error occurs.

Probable Cause 1: Injection valve not installed correctly.

Suggested Action 1: Check if the injection valve is installed correctly.

Probable Cause 2: TAG and TAG reader not aligned correctly.

Suggested Action 2: Check if the TAG or the TAG Reader are aligned correctly.

Probable Cause 3: Electrical connection or components are defective.

Suggested Action 3: Please contact your Agilent service representative.

The autosampler failed to complete the alignment correctly.

Probable Cause 1: Mechanical obstruction of the sample handler.

Suggested Action 1: Ensure unobstructed movement.

Probable Cause 2: Defective sample handler motors.

Suggested Action 2: Please contact your Agilent service representative.

The autosampler failed to complete initialization correctly, specifically related to robot movement. The processor monitors sensors and encoders during the initialization routine. If movements or status checks fail, the error occurs.

Probable Cause 1: Sample handler not aligned correctly.

Suggested Action 1: Switch off the instrument and do an autoreferencing.

Probable Cause 2: Mechanical obstruction of the sample handler.

Suggested Action 2: Ensure unobstructed movement.

Probable Cause 3: Defective sample handler motors.

Suggested Action 3: Please contact your Agilent service representative.

During initialization, the autosampler recognized the front door as open.

Probable Cause 1: Front door is not closed properly.

Suggested Action 1: Check if the front door is closed or if the magnet is missing.

During parking or movements of the needle assembly, the status information of subparts was not read out successfully.

Probable Cause 1: The sample loop capillary was squeezed in the needle parkstation.

Suggested Action 1: Check if the sample loop is installed correctly. Do an autoreferencing afterwards (needle assembly must be installed in the parkstation during this procedure).

Probable Cause 2: The needle assembly was not installed correctly in the needle parkstation.

Suggested Action 2: Check if the needle assembly is installed correctly. Install the needle assembly on the sample handler. Do a reset of the sample handler. Do an autoreferencing (the needle assembly must be installed in the parkstation during this procedure). If this does not help, please contact your Agilent service representative.

Probable Cause 3: Needle parkstation is loose.

Suggested Action 3: Carefully tighten the needle parkstation.

Probable Cause 4: For G5668A (Bio-inert Multisampler): Ceramic part of the needle is broken.

Suggested Action 4: Replace the needle assembly.

The autosampler failed to complete the injection sequence correctly; specifically, the needle hit the bottom of the vial/container.

Probable Cause 1: Sample container is not installed correctly in the pallet.

Suggested Action 1: Check if the sample container is installed correctly.

Probable Cause 2: Sample container definition in the CDS is not correct.

Suggested Action 2: Check if the correct sample container is selected in the CDS. Verify if the dimension of the sample container matches the database of your CDS.

Probable Cause 3: Sample handler not aligned correctly.

Suggested Action 3: Check if the sample handler can move freely. Do an auto referencing (needle assembly must be installed in the needle parkstation during this procedure). If this does not help, please contact your Agilent service representative.

The autosampler failed initialization because it couldn’t move the sample handler motors to their reference positions due to high current draw.

Probable Cause 1: Sample handler is blocked.

Suggested Action 1: Check if the sample handler can move freely. Switch off the instrument. Do an auto referencing (needle assembly must be installed in the needle parkstation during this procedure).

Probable Cause 2: Defective sample handler motors.

Suggested Action 2: Please contact your Agilent service representative.

The autosampler failed initialization because the electronics detected an increasing internal limit (overcurrent).

Probable Cause 1: Bad electronic connections.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Defective mainboard/fusion board.

Suggested Action 2: Please contact your Agilent service representative.

The cleaning procedure failed. The parameter indicates the type: 1 = Wash, 2 = Prime, 3 = Autoclean, 4 = Clogged seat.

Probable Cause 1: Solvent lines not installed correctly (valve block or flushpump).

Suggested Action 1: Check status of the solvent lines. Use isopropanol for verification.

Probable Cause 2: Clogged needle seat.

Suggested Action 2: Replace the needle seat.

The metering device failed to initialize correctly.

Probable Cause 1: Hydraulic box not in place.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Metering device not properly installed.

Suggested Action 2: Check the correct positioning of RFID tag and tag reader.

The flush pump device failed to initialize correctly.

Probable Cause 1: Hydraulic box not in place.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Flush pump not properly installed.

Suggested Action 2: Check the correct positioning of RFID tag and tag reader.

A peripheral valve failed to initialize correctly.

Probable Cause 1: Hydraulic box not in place.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Valve not properly installed.

Suggested Action 2: Check the correct positioning of RFID tag and tag reader.

The needle seat back flushing procedure failed.

Probable Cause 1: Clogged needle seat.

Suggested Action 1: Replace the needle seat.

The needle failed to move to the parkstation correctly.

Probable Cause 1: Autoreferencing values missing or outdated.

Suggested Action 1: Manually install the needle into the parkstation, clear current autoreferencing values (use Clear data on Lab Advisor), power cycle the module and perform autoreferencing.

The system failed to correctly remove the needle from the parkstation.

Probable Cause 1: Parkstation is loose.

Suggested Action 1: Carefully tighten the parkstation. Avoid overtightening, as this could damage the baseplate of the module.

Probable Cause 2: Needle assembly is defective.

Suggested Action 2: Replace the needle assembly.

Probable Cause 3: Autoreferencing needed.

Suggested Action 3: Manually install the needle into the parkstation, clear current autoreferencing values (use Clear data on Lab Advisor), power cycle the module and perform autoreferencing.

The system failed to remove the sample tray from the hotel position.

Probable Cause 1: Mechanical obstruction of the sample handler by reference vial holder.

Suggested Action 1: Please contact your Agilent service representative.

The index of a transport motor cannot be found. The motor ID is given in the event parameter: 0=A, 1=B, 2=Z1, 3=Z2.

Probable Cause 1: Defective fuse.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: Defective mainboard.

Suggested Action 2: Please contact your Agilent service representative.

The tag data of a transport motor cannot be read. The motor ID is given in the event parameter: 0=A, 1=B, 2=Z1, 3=Z2.

Probable Cause 1: One of the sample handler cables is not properly connected.

Suggested Action 1: Please contact your Agilent service representative.

Probable Cause 2: One of the sample handler cables is damaged (corroded or chipped off).

Suggested Action 2: Please contact your Agilent service representative.

Probable Cause 3: Defective mainboard.

Suggested Action 3: Please contact your Agilent service representative.

The peristaltic pump failed to move correctly.

Probable Cause 1: Pump tubing blocked.

Suggested Action 1: Verify that the solvent tubing is not blocked.

Probable Cause 2: Pump motor is defective.

Suggested Action 2: Replace the pump motor.

Probable Cause 3: Pump is defective.

Suggested Action 3: Replace the peristaltic pump.

The compressor voltage is below the lower threshold value.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The cooler/thermostat was switched off due to a condensate event.

Probable Cause 1: Overfilled container.

Suggested Action 1: Empty the condensate container. Verify that the open end of the tubing doesn’t immerse in the liquid.

Probable Cause 2: Drainage issues.

Suggested Action 2: Verify the correct plumbing of the condensate drainage system. Make sure that no kinks or mechanical blocks are present in the drainage system. Avoid the formation of the siphoning effect. Make sure that the hosting sampler is level.

The pressure in the refrigerant circuit exceeded the maximum allowed level, and the compressor was turned off to prevent damage.

Probable Cause 1: Overheated condenser.

Suggested Action 1: Turn off the cooler/thermostat and wait 15 min for the system to cool down. Verify adequate space around the sampler for ventilation and ensure the cooler/thermostat is not exposed to direct sunlight.

Probable Cause 2: Potential hardware error.

Suggested Action 2: Please contact your Agilent service representative.

The system is in an error state because the calibration of the analog temperature sensor failed.

Probable Cause 1: Sampler incompatibility.

Suggested Action 1: If the hosting sampler is a Vialsampler, verify compatibility with the Sample Cooler. Units with serial number DEBAT02000 or below have an analog sensor incompatible with the Vialsampler.

Probable Cause 2: Potential hardware error.

Suggested Action 2: Please contact your Agilent service representative.

The compressor turned off due to an unexpected drop in the supply voltage.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The condensate sensor of the cooler/thermostat is not working properly.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The system is in an error state because the compressor control board encountered an unexpected error.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The condenser fan of the cooler/thermostat is not working properly.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The system is in an error state because communication between the sampler and the thermostat failed.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The heating function of the thermostat is not working properly.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The system is in an error state because the thermostat heater encountered an unexpected error during operation.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

One of the digital temperature sensors of the cooler/thermostat is not working properly.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The system is in an error state because the control board of the compressor encountered an unexpected error.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

The sample thermostat type is unknown or unrecognized.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

One of the cooling fans of the cooler/thermostat is not working properly.

Probable Cause 1: Potential hardware error.

Suggested Action 1: Please contact your Agilent service representative.

Chapter 8 describes the built-in test functions.

All tests are described based on the Agilent Lab Advisor Software B.02.06 or above. Other user interfaces may provide fewer or no tests.

For details on the use of the interface, refer to the interface documentation.

Interface: Agilent Lab Advisor

Comment: All tests are available. Adding pressure to chromatographic signals possible.

Available Functions:

System Pressure test

Maintenance

Drawer Detection/Auto Referencing

Sample Cooler Function Test

Sample Thermostat Function Test

Sample Handler Function Test

Interface: Agilent ChemStation

Comment: No tests available. Adding pressure to chromatographic signals possible.

Available Functions:

Drawer Detection/Auto Referencing

Temperature mainboard

Pressure/Pressure ripple

The test determines the leak rate of the system between the pump outlet valves and a blank nut.

The blank nut can be positioned at different locations before the flow cell to determine and verify the leak rate of individual modules and components.

It allows setting the test pressure. It is recommended to perform the test at a pressure corresponding to the normal operating pressure of the system, as leak rates are not always linear.

In case of a suspected leak.

To verify successful execution of maintenance.

Part Number: 5067-6127, Description: Blank Nut SL. For the 1290 Infinity II Multisampler, the Blank Nut SL must be used as it fits the special port size of the VICI valve. This nut is backward compatible and can also be used for the 1260 Infinity II Multisampler.

Run the System pressure test using the Agilent Lab Advisor software. Follow the steps provided in the test procedure within the software (Prepare pump pressure test, Enter test pressure, Flush system, Check pump leak rate, Insert blank nut, Check system leak rate, Evaluate results, Restore configuration). Input the desired test pressure when prompted.

Check the probable causes and perform suggested actions:

Probable Cause 1: Damaged blank nut (poorly shaped from over-tightening).

Suggested Action 1: Ensure the blank nut is in good condition and properly tightened before investigating other sources.

Probable Cause 2: Pump leakages.

Suggested Action 2: Perform the Pump Head Leak test.

Probable Cause 3: Loose or leaky fittings.

Suggested Action 3: Tighten the fittings or replace capillaries.

Probable Cause 4: Autosampler leakages.

Suggested Action 4: Perform the Autosampler Leak test.

Probable Cause 5: Thermostatted Column Compartment valve leakages.

Suggested Action 5: Replace the TCC valve rotor seal.

An ‘error’ is caused by an abnormal termination during the test operation. A ‘failed’ result indicates that the test result was not within the specified limits.

Auto referencing uses predefined positions on the base plate and sample hotel to calibrate the positioning of the needle parkstation and sample hotel.

It compensates for deviations in positioning the needle assembly and sample tray.

After disassembling the module, or when exchanging the sample handler, sample hotel, needle parkstation, needle assembly, or one of the main boards. It is also implemented in the drawer detection and needle exchange routines.

Workspace of the multisampler is empty.

All drawers are closed properly.

All drawers have two sample trays installed, but no sample containers.

All drawers have been properly configured.

Needle assembly is installed in the needle parkstation.

1. Open the CDS of the instrument.

2. Right-click in the Active Area of the Multisampler to open the Modify menu.

3. Select ‘Drawer Configuration’, ‘Right Capillaries’, or ‘Reference Vial Array’.

4. Follow the software instructions displayed for the chosen configuration option. Auto referencing is performed as part of these procedures.

5. After completion, click the ‘Back’ button to leave the Service & Diagnosis menu if applicable.

Yes, alternatively, the Local Controller can be used for auto referencing.

In the Agilent Lab Advisor Software, the maintenance positions can be selected in the Service & Diagnostics view. To perform maintenance on the module, run the Maintenance Positions in the Service & Diagnostics View in the Agilent Lab Advisor (for further information see Online-Help of user interface).

The “Change Needle Assembly” maintenance position moves the Sample handler to position the needle assembly for easy access when changing the needle assembly or needle seat. The position is far to the left of the needle parkstation, and the current to the motors are off, allowing the Z-drive of the robot to be moved while servicing the module.

For safety reasons, you must lock the needle assembly before you detach the needle from the robot. Refer to “Remove the Needle Assembly” and “Install the Needle Assembly” procedures for details.

No, during normal operation the needle assembly has to be unlocked.

The Change Loop command positions the Z-drive of the robot arm far to the left of the needle parkstation to enable easy exchange of the sample loop cartridge.

The home position of the multisampler ensures better access to the workspace. When transporting the module, it is highly recommended to use the Instrument Control > Park Position command to place the Sample Handler in a position for safe transport.

If the transport assembly is not parked and not protected by the transport foam, the module could be damaged due to excessive shock of the shipping container during transport.

The “Change Metering Device” maintenance position should be used when removing the metering device is necessary, for instance, when exchanging the metering seal.

This command moves the metering drive to a position at the far back to prevent seal and/or piston damage during removal or maintenance of the metering device.

Injector Steps allow each movement of the sampling sequence to be done under manual control. This is useful during troubleshooting, where close observation of each sampling step is required to confirm a specific failure mode or verify successful completion of a repair. Each injector step command consists of a series of individual commands that move the multisampler components to predefined positions.

Run the Injector Steps in the Service & Diagnostics View in the Agilent Lab Advisor (for further information see Online-Help of user interface).

Select the individual step command required, such as needle selection and needle position. Use the interface controls for Tray Selection, Needle Selection, Needle Position, Draw Parameters, and Valve settings. Follow the action/result log to track commands. It is important to follow a logical order when using the injector steps function.

The Sample Cooler Function Test is used as a simple verification that the Sample Cooler is functioning correctly.

After the test starts, it acquires data from the cooler’s PT1000 temperature sensor. Once the temperature equilibrates (changes by less than 0.5 °C over 10 seconds), the cooler is turned on, and measurement begins. For the test to succeed, three temperature checkpoints must be reached within a specified time. If a Sample Thermostat with a heating function is available, the electrical resistance is also checked.

The Sample Handler Function Test is designed to check that the Multisampler’s sample handler unit operates as expected. The test collects current and position signals while the arm moves, comparing the data to built-in limits to verify if the sample handler is defective. The result screen indicates Passed or Failed, providing reasons and comments for any errors.

This test should be run in case of failed auto referencing or errors related to the sample handler.

Before running the test, ensure the following conditions are met:

Workspace of the multisampler is empty

All drawers are closed properly

All drawers have two sample trays installed, but no sample containers

Needle assembly is installed in the needle parkstation

Pumps are turned off

1. Run the Sample Handler Function Test using the Agilent Lab Advisor (refer to the Online-Help of the user interface for more details).

2. In the test dialog, check all items in the preparation checklist to confirm the conditions are met. The test can only start once all boxes have been checked.

3. Click Start.

Click the Back button to leave the Service & Diagnostics menu.

This chapter describes the maintenance of the Multisampler, including:

Introduction to Maintenance

Warnings and Cautions

Overview of Maintenance

Clean the Module

Removal and Installation of the Front Door

Remove the Needle Assembly

Install the Needle Assembly

Exchange the Needle Seat

Replace the Rotor Seal

Replace the Injection Valve

Remove the Metering Seal

Install the Metering Seal

Replace the Peristaltic Pump Cartridge

Replace the Flushhead Seal

Remove the Sample Loop-Flex

Installing the Sample Loop-Flex

Replace the Dummy Drawer

Optional Configurations (Installing and Replacing Drawers, Configuration of Hotel Drawers)

Replace the Sample Cooler/Sample Thermostat

Replace the Module Firmware

The main user-accessible assemblies that can be accessed from the front for simple repairs on a standard multisampler are:

Door

Peristaltic pump

Metering device

Dummy drawer

Needle assembly

Drawer

Needle seat

Injection valve

The main user-accessible assemblies for a multiwash multisampler are:

Door

SSV/piezo pump

Flush head

Metering device

Dummy drawer

Needle assembly

Drawer

Needle seat

Injection valve

Agilent is not responsible for any damages caused, in whole or in part, by improper use of the products, unauthorized alterations, adjustments or modifications to the products, failure to comply with procedures in Agilent product user guides, or use of the products in violation of applicable laws, rules or regulations. Use your Agilent products only in the manner described in the Agilent product user guides.

Repair work at the module can lead to personal injuries, e.g., shock hazard, when the cover is opened. Do not remove the cover of the module. Only certified persons are authorized to carry out repairs inside the module.

Sharp-edged parts of the equipment may cause injuries. To prevent personal injury, be careful when getting in contact with sharp metal areas.

The handling of solvents, samples and reagents can pose health and safety risks.

When working with these substances, observe appropriate safety procedures (e.g., wearing goggles, safety gloves, protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

The volume of substances should be reduced to the minimum required for the analysis.

Do not operate the instrument in an explosive atmosphere.

If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

Metal parts in the flow path can interact with bio-molecules, leading to sample degradation and contamination.

For bio-inert applications, always use dedicated bio-inert parts, identifiable by the bio-inert symbol or other markers described in the manual.

Do not mix bio-inert and non-inert modules or parts in a bio-inert system.

It is necessary to perform periodic inspections to ensure safe use. These can be performed by Agilent service representatives on a contractual basis.

To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent.

Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module.

Do not use an excessively damp cloth during cleaning.

Drain all solvent lines before opening any connections in the flow path.

The front door should be removed or replaced if it is defective or a hinge is damaged.

Tools required: Flat screwdriver

Parts required: #1 p/n 5067-5415 Door Assy OR #1 p/n G7167-68718 Light Protection Kit

Preparations: Finish any pending acquisition job and return any plate on the workspace back to the hotel.

Magnetic Fields: Magnets produce a far-reaching, strong magnetic field. They can damage items like televisions, laptops, computer hard disks, credit cards, and magnetic cards. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

Heart Pacemakers: Magnets could affect the functioning of pacemakers and implanted heart defibrillators. A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. Bearers of heart pacemakers or implanted defibrillators must stay off at least 55 mm from the magnets.

Removal:

1. Open the front door.

2. Press the release buttons and pull the front door out.

Installation:

3. Insert the hinges into their guides and move the door in until the release buttons click into their final position.

Remove the needle assembly when the limit in the needle into seat counter in the EMF is exceeded, or when the needle shows indications of damage, blockage, or leaks.

Note: For bio-inert modules use bio-inert parts only!

Tools required: p/n 8710-0510 Open-end wrench 1/4 – 5/16 inch

Parts required: #1 p/n G4267-87201 Needle Assembly OR #1 p/n G4267-87210 Needle Assembly (slotted) for high injection volumes OR #1 p/n G5668-87200 Needle Bio-Sampler (for G5668A)

Preparations: In order to avoid leaks, stop the pump running and remove the tubings from the solvent bottles. If available close the shutoff valves.

An uncovered needle is a risk of harm to the operator.

Do not open the safety lock of the needle assembly.

Be careful working at the z-robot.

Wear safety goggles when removing the needle assembly.

The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Yes, it is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage.

1. Start the maintenance mode: In the Local Controller, select the Change Needle, Loop and Seat function. OR In the Agilent Lab Advisor software, select Service & Diagnostics > Maintenance Positions > Change Needle, Loop and Seat, click Start and wait until the needle assembly is in the maintenance position.

2. Open the front door.

3. Lock the needle in the safety position. WARNING: Sharp needle. Uncovered needles may cause injuries. Make sure the needle is in the safety lock position.

4. Remove the needle assembly by slightly pulling the needle cartridge.

5. The Z-Robot (Z-arm coupler) is now without the needle assembly.

6. The needle assembly is still connected to the loop capillary via the loop plastic adapter. CAUTION: Damage of the loop. The loop shape may be damaged if the loop is stretched or bent too far. Avoid changing the loop shape. Do not pull or bend the loop too far.

7. Remove the loop plastic adapter following the sequence shown in the illustration. NOTE: Do not open the rear plastic clamp. NOTE: If the plastic adapter is damaged the sample loop has to be replaced.

8. Use a 1/4 inch wrench to loosen the fitting of the loop capillary.

9. Remove the needle assembly.

Note: For bio-inert modules use bio-inert parts only! It is recommended to always exchange the needle assembly and the needle seat at the same time.

1. Install the loop capillary on top of the needle cartridge (1.) and tighten the fitting hand tight (2.). NOTE: If the sample loop is changed, we recommend changing the needle as well.

2. Use a 1/4 inch wrench to tighten the fitting of the loop capillary. CAUTION: Blockages inside of the needle assembly union. Do not overtighten the fitting. A quarter turn should be sufficient.

3. Install the loop plastic adapter following the sequence in the illustration. NOTE: Verify the sample loop info on the plastic adapter. A left or a right sample loop must be installed in the correct slot of the needle parkstation. For single needle, the default position is on the right. NOTE: If the plastic adapter is damaged the sample loop has to be replaced.

4. Pinch and reinsert the needle assembly and the connected loop capillary into the z-arm coupler. NOTE: Check the tension of the loop capillary. This must be forced and guided to the hydraulic box to prevent it from being caught by the Z-drive.

5. Close the front door.

Next Steps:

6. Exit maintenance mode: In the Local Controller close Change needle /seat. OR In the Agilent Lab Advisor software Change needle/loop> End, click End and wait until the needle assembly is in the needle park station.

7. Perform a pressure test.

Exchange the needle seat when it is visibly damaged, blocked, or leaks.

Note: For bio-inert modules use bio-inert parts only!

Tools required: p/n 8710-0510 Open-end wrench 1/4 – 5/16 inch, Flat head screwdriver

Parts required: #1 p/n G4267-87012 High Pressure Needle Seat, 0.12 mm (PEEK) OR #1 p/n G4267-87020 High Pressure Seat Assembly 0.075 mm (PEEK) OR #1 p/n G5668-87017 Bio Seat ID 0.17 (for G5668A)

Preparations: In order to avoid leaks, stop the pump running and remove the tubings from the solvent bottles. If available close the shutoff valves. Refer the Agilent 1290 Infinity II Ultra Low Dispersion Kit Technical Note (p/n 01200-90105) for further details.

WARNING: Risk of injury by uncovered needle. An uncovered needle is a risk of harm to the operator. Do not open the safety lock of the needle assembly. Be careful working at the z-robot. Wear safety goggles, when removing the needle assembly.

1. Start the maintenance mode: In the Local Controller start the maintenance mode and select Change needle/seat function. OR In the Agilent Lab Advisor software select Service & Diagnostics in the system screen Maintenance Positions> Change Needle, click Start and wait until the needle assembly is in maintenance position.

2. Open the front door.

3. Disconnect the seat capillary from the Injection valve.

4. Slightly pull (1.) the front clip which holds the needle seat in position. Then carefully lift up (2.) the complete leak tube needle assembly from the holder.

5. Insert the new Needle seat (1.). Press it firmly in position (2.). NOTE: Verify that the needle seat clip is locked in the needle park station.

6. Reconnect the seat capillary to the injection valve.

7. Close the front door.

Next Steps:

8. Exit maintenance mode: In the Local Controller close Change needle /seat. OR In the Agilent Lab Advisor software Change needle click End and wait until the needle assembly is in the needle park position.

9. Perform a pressure test.

Replace the rotor seal when there is poor injection volume reproducibility or when the injection valve is leaking.

Note: For bio-inert modules use bio-inert parts only! Please bear in mind that depending on which valve you have installed the images may slightly differ from the actual item.

Tools required:

• p/n 8710-0510 Open-end wrench 1/4 – 5/16 inch
• p/n 8710-2394 Hex key 9/64 inch 15 cm long T-handle
• Cleaning tissue and appropriate solvent like isopropanol or methanol

Parts required (select appropriate):

• #1 p/n 5068-0198 Rotor Seal 1300 bar (PEEK) for 1290 Infinity II Injection Valve
• #1 p/n 5068-0209 Rotor Seal (PEEK) for 1260 Infinity II Injection Valve and Bio-inert injection valve
• #1 p/n 5068-0229 Rotor Seal (PEEK) for 3Pos/6Port Peripheral Valve Dual Needle
• #1 p/n 5068-0232 Rotor Seal (PEEK) for 2Pos/8Port Injection Valve Dual Needle
• #1 p/n 0100-1851 Stator face, ceramic for the bio-inert injection valve

CAUTION: Component cleanliness is crucial for the life time of the injection valve. Replace the rotor seal in a clean environment.

1. Open the front door.

2. Remove all capillaries from the injection valve with a 1/4 inch wrench. NOTE: Remember the correct plumbing. Check the drawing on the side cover of the hydraulic box for correct plumbing.

3. Use a 9/64 inch hex driver to unscrew the two socket screws which hold the stator head in place.

4. Carefully remove the stator head. CAUTION: Damage to the stator head. The polished sealing surface can be easily damaged. Avoid touching the polished surface. Never place the polished surface on a hard surface. To ensure that the sealing surface of the stator head is not damaged, place it on its outer face.

5. Remove the rotor seal. Use a small tool to gently pry the rotor seal away from the drive. NOTE: Examine the rotor sealing surface for scratches and nicks. If scratches are visible the rotor seal must be replaced. If no scratches are visible clean all the parts with an appropriate solvent, taking care that no surfaces get scratched.

6. Install the new rotor seal. CAUTION: Damage to the rotor seal and cross-port leaks. Before you replace the rotor seal, clean the stator. Inspect the stator head and swab it with the appropriate solvent. If more stringent cleaning is required, use a sonicator. Inspect the remaining valve components for contamination. Clean them as necessary. If the stator head is scratched, replace it. NOTE: Make sure that the rotor sealing surface with its engraved flow passages is facing out. The pattern is asymmetrical to prevent improper placement. NOTE: The Bio-inert injection valve additionally has a stator face installed.

7. Reinstall the stator head. The index pins on the drive and the stator head must engage in the corresponding holes. Insert the two socket head screws.

8. Using a 9/64 in. L-Hex wrench, tighten each screw gently until you feel resistance (approximately fingertight). Tighten each screw by 1/8 turn, and then tighten each screw again, until the stator is secured to the driver. NOTE: Do not over-tighten the screws. The screws hold the assembly together and do not affect the sealing force. The sealing force is automatically set as the screws close the stator head against the valve body.

9. Reconnect all capillaries to the proper injection valve ports with a 1/4 inch wrench.

10. Close the front door.

11. Perform a pressure test.

Replace the injection valve when adding a new injection valve or if the existing one is defective.

Note: For bio-inert modules use bio-inert parts only! Please bear in mind that depending on which valve you have installed the images may slightly differ from the actual item.

Tools required: Wrench 9/64

Parts required (select appropriate):

• #1 p/n 5067-4232 2pos/6port Injection Valve (VICI) 1300 bar (G7167B)
• #1 p/n 5067-6698 2ps-6pt RC Injection Valve 800 bar (G7167A)
• #1 p/n 5067-4260 2pos/8port Injection Valve Dual Needle 1300 bar
• #1 p/n 5067-4263 2pos/6port Injection Valve Bio-inert 600 bar for bio inert solution

Preparations: Switch off the power of the Multisampler.

1. Disconnect the capillaries.

2. Turn the spanner nut counter clockwise until the injection valve head detaches from the hydraulic box (Do not use wrenches on the spanner nut).

3. Remove the spanner nut from the injection valve head.

4. Take the replacement injection valve head and insert it into the open actuator slot of the hydraulic box. Rotate until the unions at the base of the replacement injection valve head and the valve actuator engage. OR If the outside pin does not fit into the outside groove, you have to turn the valve head until you feel that the two pins snap into the grooves. Now you should feel additional resistance from the valve drive while continue turning the valve head until the pin fits into the groove. NOTE: Check the orientation of the rear side. Verify the correct position of the Valve TAG.

5. Continue to rotate until the clocking pin in the injection valve head align with the notch in the housing and press the replacement injection valve head into the actuator.

6. Replace the spanner nut (1.) and tighten clockwise (2.). Hand tighten only, do not use wrenches on the spanner nut.

7. Reconnect the capillaries.

Remove or replace the metering seal when experiencing poor injection volume reproducibility or when the metering device / analytical head is leaking.

Note: For bio-inert modules use bio-inert parts only!

Tools required:

• p/n 8710-0510 Open-end wrench 1/4 – 5/16 inch
• p/n 8710-2392 4 mm Hex key
• p/n 01018-23702 Insert tool OR p/n G4226-43800 Seal insert tool for 100 µL or 40 µL

Parts required (select appropriate seal, piston if needed):

• #1 p/n 0905-1717 Metering Seal, 40 µL
• #1 p/n 0905-1719 Metering Seal, 100 µL
• #1 p/n G5611-21503 Metering Seal PTFE (Bio-inert) for bio inert solution
• #1 p/n 5067-5920 Piston, 40 µL, Zirconia (If previous piston is scratched for 40 µL head)
• #1 p/n 5067-5678 Piston, 100 µL, Zirconia (If previous piston is scratched for 100 µL head)

1. Enter maintenance mode: In the Local Controller start the maintenance mode and select Change metering device function. OR In the Agilent Lab Advisor software select Service & Diagnostics in the system screen (Tools)> Maintenance Positions> Change Metering Device, click start and wait until the metering device is in maintenance position.

2. Open the front door.

3. Disconnect all capillaries from the metering device.

4. To release the bayonet lock, push (1.) and rotate (2.) the analytical head a quarter turn left. Then you can pull and detach the analytical head assembly from the actuator (3.).

5. Remove the metering device.

6. Take the metering device. Push against the rear side of the metering device and rotate a quarter left to release the bayonet lock.

7. Now you can separate the analytical head and head body.

8. Remove the piston out of the head body.

9. Inspect the piston for cleanliness and scratches. If dirty: Clean the piston with an appropriate solvent. If scratched: Replace the piston by a new one.

10. Take the analytical head and remove the three screws on the rear side, which holds the support ring in place. Check the support ring for any damages.

11. Carefully remove the metering seal using the steel side of the insert tool. Clean the chamber with an appropriate solvent and ensure that all particulate matter is removed.

Note: For bio-inert modules use bio-inert parts only!

Preparations: Complete the steps for “Remove the Metering Seal”.

1. Install the new metering seal using the plastic side of the insert tool. Press it firmly into position. Avoid any offset angle as it might deform the seal.

2. Reassemble the support ring.

3. Make sure to comply to the following order of actions: a. Tighten the three screws fingerthight, then b. Tighten the screws a little at a time to keep the support ring surface parallel (important!) to the surface of the analytical head.

4. Use the twist and lock bayonet mechanism to reassemble the analytical head assembly. Push the two parts together to couple the head body with the analytical head. Once the pin reaches the bottom of the slot, one or both parts are rotated so that the pin slides along the horizontal arm of the L until it reaches the serif. The spring then pushes the male connector up into the serif to keep the pin locked into place.

5. Press the piston carefully into the housing of the head body and the seal.

6. Reinstall the complete analytical head with the actuator housing. NOTE: For proper installation, check the correct position of the tag.

Follow these steps to install the metering seal:

1. Fix the analytical head by pushing (1.) and rotating (2.) via the twist and lock bayonet mechanism.

2. Reconnect the capillaries.

3. Close the front door.

Next Steps:

4. In the Local Controller exit the maintenance mode and select Change metering device function.

OR

In Agilent Lab Advisor software system screen exit Service & Diagnostics (Tools)> Maintenance Positions> Change Metering Device click End and wait until the metering device is in Home position.

5. Perform a pressure test.

Replace the Peristaltic Pump Cartridge when the tubing is blocked or broken.

Parts required:

Preparations:

To avoid spilling of solvents, remove the solvent lines from the bottles.

WARNING: When opening capillary or tube fittings solvents may leak out. The handling of toxic and hazardous solvents and reagents can hold health risks.

✓ Please observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used.

NOTE: The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not replaceable.

1. Open the front door.

2. Press the two clips on the front of the peristaltic pump cartridge.

3. Pull the cartridge forward off the motor shaft.

4. Disconnect the tubing coupler leading to the wash port and the tubing coupler coming from the solvent bottle.

5. Push the new cartridge onto the motor shaft until the clips click into place.

6. Connect the wash port tubing to the upper tubing of the new cartridge (use sand paper to get a good grip on the tubing).

7. Connect the inlet filter of the solvent bottle again. Use the syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump.

8. Close the front door.

Replace the Flushhead Seal when the flush head is leaking.

NOTE: For bio-inert modules use bio-inert parts only!

Tools required:

Parts required:

Preparations:

• Cleaning tissue

• Appropriate solvent like isopropanol or methanol

1. In the Local Controller start the maintenance mode and select Change metering device function.

OR

In the Agilent Lab Advisor software select Service & Diagnostics in the system screen (Tools)> Maintenance Positions> Change Metering Device, click start and wait until the metering device is in maintenance position.

2. Open the front door.

3. Remove capillaries and valves from the flush head.

4. Press and turn the Flush Head a quarter left (bayonet fitting) and detach the metering device from the actuator.

5. Pull the flush head away from the hydraulic box.

6. Press against the rear side of flush head and turn a quarter left (bayonet fitting) and separate the flush head, head body and the piston.

NOTE: Be careful not to break the piston.

7. Remove the piston from the head body.

8. Carefully remove the metering seal from the tip of the piston.

9. Reassemble the flush head and the head body (without piston).

10. Carefully insert the piston with the new metering seal into the flush head assembly.

11. Reinstall the flush head to the actuator housing.

NOTE: For proper installation, check the correct position of the tag.

12. Fix the flush head.

13. Connect the capillaries.

14. Close the front door.

Remove or replace the Sample Loop-Flex if it is defective or damaged.

NOTE: For bio-inert modules use bio-inert parts only!

Tools required:

Parts required:

NOTE: Further sample loops for the Dual Needle option are available, see “Sample Loops and Capillaries (Dual Needle)”.

Preparations:

Finish any pending acquisition job and return any plate on the workspace back to the hotel.

WARNING: Risk of injury by uncovered needle. An uncovered needle is a risk of harm to the operator.

✓ Do not open the safety lock of the needle assembly

✓ Be careful working at the z-robot.

✓ Wear safety goggles, when removing the needle assembly.

1. In the Local Controller start the maintenance mode and select Change Needle, Loop and Seat function.

OR

In the Agilent Lab Advisor software select Service & Diagnostics in the system screen Maintenance Positions> Change Needle, Loop and Seat, click Start and wait until the needle assembly is in maintenance position.

2. Open the front door.

3. The needle assembly is still connected to the loop capillary. Use a 1/4 inch wrench to loosen the fitting of the loop capillary connected to the analytical head.

4. Lock the needle in the safety position.

NOTE: During normal operation of the Multisampler the needle assembly has to be unlocked.

CAUTION: Damage of the loop. The loop shape may be damaged if the loop is stretched or bent too far.

✓ Avoid to change the loop shape.

✓ Do not pull or bend the loop too far.

WARNING: Sharp needle. Uncovered needles may cause injuries.

✓ Make sure the needle is in the safety lock position.

5. Remove the needle assembly by slightly pulling the needle cartridge.

6. Remove the cartridge out of its proper position. By gently tilting and pulling it out of the work space of the multisampler.

7. Remove the loop plastic adapter following the sequence (1.) through (4.) indicated in the diagram.

NOTE: Do not open the rear plastic clamp.

NOTE: If the plastic adapter is damaged the sample loop has to be replaced.

8. Use a 1/4 inch wrench to loosen the fitting of the loop capillary.

9. Remove the needle assembly.

CAUTION: Mismatching sample loop configuration. Damage to the system.

✓ Make sure, that the sample loop configuration matches to the hardware installed.

NOTE: If you have changed the sample loop, verify that the correct sample loop is configured in the CDS.

NOTE: For details on the setup of the dual-needle system, refer to “Modify Capillaries”.

1. Install the loop capillary on top of the needle cartridge (1.) and tighten the fitting hand tight (2.).

CAUTION: Blockages inside of the needle assembly union.

✓ Do not overtighten the fitting. A quarter turn should be sufficient.

2. Then use a 1/4 inch wrench to tighten the fitting of the loop capillary.

NOTE: If the sample loop is changed, we recommend changing the needle as well.

3. Install loop plastic adapter following the sequence (1.) through (4.) indicated in the diagram.

NOTE: Verify the sample loop info on the plastic adapter. A left or a right sample loop must be installed in the correct slot of the needle parkstation. For single needle, the default position is on the right.

NOTE: If the plastic adapter is damaged the sample loop has to be replaced.

4. Click the sample loop cartridge in the designated location and keep the right orientation.

5. Install the shorter capillary of the sample loop cartridge to the analytical head.

6. Pinch and reinsert the needle assembly and the connected sample loop capillary into the z- arm coupler.

NOTE: Check the tension of the loop capillary. This must be forced and guided to the hydraulic box to prevent it from being caught by the Z-drive.

7. Close the front door.

Next Steps:

8. In the Local Controller close Change needle /seat.

OR

In Agilent Lab Advisor software Change needle/loop. Click NEXT and wait until the needle is in the needle park station. Click Back to leave the Maintenance window.

NOTE: If you need an autoreferencing step included you must choose the change needle procedure.

NOTE: If you have changed the sample loop, verify that the correct sample loop is configured in the CDS.

Table 26: Overview on optional configurations (examples for uniform types)

NOTE: Mixed configurations are possible (for example 1x3H- with 1x2H- and 3x1H-drawer).

NOTE: All positions in the Sample Hotel must be filled either with dummies or drawers. The drawers must be installed from bottom to top.

Tools required:

• Screwdriver

Parts required:

NOTE: Before you start the new drawer installation you have to remove the lower drawer (2H drawer = default configuration) from the Sample Hotel.

NOTE: For best cooling performance the 2H drawer must be installed in the lowest position.

NOTE: More detailed video information is available on the Agilent Information Center.

1. Open the drawer.

2. Pull the drawer completely out.

3. Unlatch the drawer: Use a screwdriver to press the clamping lever lightly to the left.

4. Remove the drawer from the rail guide. The drawer is now out of the hotel.

5. If replacing a dummy drawer: Grab in the recession below the dummy drawer front panel (1.) and lift the left side (2.).

6. Remove the dummy drawer.

NOTE: At this stage remove all other dummies that will be replaced by hotel drawers.

7. Place the new drawer horizontally into the sample hotel. Check that the drawer matches the middle bracket of the sample hotel.

8. Push until the complete drawer locks in place.

NOTE: Take care that the clamping lever locks.

NOTE: Always fill sample hotel completely (no empty drawer slots). Otherwise the drawers can’t be configured in the software.

9. Configure the hotel drawers in the controller software (see the Online Help of the software for details).

The configuration of your drawers is necessary to detect the new drawer configuration for your CDS system. When a wrong configuration is detected there will be a mismatch in your CDS system and you are not able to use the new drawers. The new drawer configuration is active and stored after you have done the Drawer Configuration.

Software required:

• OpenLAB (A.02.01 or above)

• LC driver (A.02.10 or above)

Preparations:

• Stop the acquisition run.

• Remove the sample containers (trays and well plates) from workspace.

• Complete the drawer installation.

• Remove the sample containers (trays and well plates) from the drawers.

• Verify that all sample trays (palettes) are installed in their drawers.

• All open drawers and dummies have to be closed and installed properly.

Procedure:

1. Start OpenLAB CDS ChemStation Edition.

2. Right-click on the Multisampler GUI.

3. Select Modify > Drawer Configuration in the GUI screen.

NOTE: For correct detection, it is necessary to remove all sample containers (for example 54 vial tray or well plates).

4. Follow the Setup or Change configuration screen.

5. System is ready after the robot has done Auto Referencing.

Software required:

• Lab Advisor (B.02.05 or above)

Preparations:

• Stop the acquisition run.

• Remove the sample containers (trays and well plates) from workspace.

• Complete the drawer installation.

• Remove the sample containers (trays and well plates) from the drawers.

• Verify that all sample trays (palettes) are installed in their drawers.

• All open drawers and dummies have to be closed and installed properly.

Procedure:

1. Start the Lab Advisor Software.

2. Connect the instrument and select Instrument Control in the system screen.

3. Switch In the Configuration menu of the Multisampler. Select Detect Drawers in the Hotel Configuration.

4. Follow the Detect Hotel Configuration screen to detect the physically available drawers.

NOTE: For correct detection, it is necessary to remove all sample containers (for example 54 vial tray or well plates).

5. System is ready after the robot has done Auto Referencing.

Replace the Sample Cooler/Sample Thermostat if it is damaged or defective.

Tools required:

Parts required:

WARNING: Flammable refrigerant. Formation of flammable gas-air mixtures inside the Sample Thermostat and laboratory.

✓ Keep open fire or sources of ignition away from the device.

✓ Ensure a room size of 4 m³ (1 m³ for every 8 g of R600a refrigerant inside of the Sample Thermostat).

✓ Ensure adequate ventilation: typical air exchange of 25 m³/h per m² of laboratory floor area.

✓ Keep all ventilation openings in the enclosure clear of obstructions. Do not block the openings on the circumference of the Sample Thermostat.

WARNING: Flammable refrigerant used.

✓ When handling, installing and operating the Sample Thermostat, care should be taken to avoid damage to the refrigerant tubing or any part of the Sample Thermostat.

WARNING: In the event of a damage:

✓ Keep open fire or sources of ignition away from the device.

✓ Ventilate the room for several minutes.

✓ Do not use the Sample Thermostat any more.

WARNING: Heavy weight. The module is heavy.

✓ Carry the module at least with 2 people.

✓ Avoid back strain or injury by following all precautions for lifting heavy objects.

✓ Ensure that the load is as close to your body as possible.

✓ Ensure that you can cope with the weight of your load.

CAUTION: Routing of the condensation tubing. Proper routing of the condensation tubing is critical for correct condensate drainage.

✓ Do not place the sampler directly on the bench.

CAUTION: Condensate inside the cooler or thermostat. Damage to the electronics.

✓ Unplug the power cords.

✓ Drain off all condensate before dismounting the sample cooler or thermostat.

✓ Make sure that there is no condensate left.

1. Ensure that the power switch on the front of the module is OFF (switch stands out).

2. Disconnect the power cable from the sampler.

3. Ensure that no condensate remains inside the cooler/thermostat before proceeding forward.

NOTE: Gently tapping on the sides of the sampler can help to remove the last traces of condensate from the system.

4. Remove the condensate tubing.

NOTE: If there is still some condensate inside the cooler/thermostat, place a suitable container underneath the outlet pipe and keep tapping on the sides of the sampler until no water is coming out.

5. Remove the fixation screws on the back of Sample Cooler/Sample Thermostat.

6. Pull the cooler/thermostat halfway out, disconnect the power and the data cable and then remove the unit completely from the sampler.

7. Slide the new cooler/thermostat halfway into the sampler and connect the power and the data cable.

CAUTION: Damage to the cables.

✓ Do not bend or pinch the cables.

✓ Make sure that the Sample Cooler/Sample Thermostat fits perfectly in the sampler.

8. Slide the cooler/thermostat all the way into the sampler, making sure that the cables don’t get jammed between the metal parts.

9. Fix the unit with the four screws.

10. Reconnect the condensate tubing.

NOTE: For information on proper condensate handling, refer to “Install the Sample Cooler/Sample Thermostat”.

11. Connect the power cable to the power connector at the rear of the module.

CAUTION: Damage to the Sample Cooler/Sample Thermostat.

✓ Wait at least 30 min before switching on the compressor of the cooler/thermostat.

✓ This allows the refrigerant and system lubrication to reach equilibrium.

12. Switch on the sampler and perform the Sample Cooler Function Test to verify the correct functioning of the new cooler/thermostat.

When to Update/Downgrade Firmware:

• Installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same (validated) revision.

• Installation of older firmware might be necessary to keep all systems on the same (validated) revision or if a new module with newer firmware is added to a system or if third party control software requires a special version.

Tools required:

• Agilent Lab Advisor software

Parts required:

• Firmware, tools and documentation from Agilent web site

Preparations:

• Read update documentation provided with the Firmware Update Tool.

Procedure:

To upgrade/downgrade the module’s firmware carry out the following steps:

1. Download the required module firmware, the latest FW Update Tool and the documentation from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761

2. For loading the firmware into the module follow the instructions in the documentation.

Module Specific Information:

There is no specific information for this module.

The general safety precautions listed in the manual must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements.

Ensure the proper usage of the equipment. The protection provided by the equipment may be impaired if not used as specified.

The operator of this instrument is advised to use the equipment in a manner as specified in this manual.

Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Make all connections to the unit before applying power.

Note the instrument’s external markings described under “Symbols”.

Missing electrical ground can lead to electrical shock.

If your product is provided with a grounding type power plug, the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard.

The ground pin must be firmly connected to an electrical ground (safety ground) terminal at the power outlet. Any interruption of the protective (grounding) conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury.

Removing instrument covers can lead to electrical shock.

Do Not Remove the Instrument Cover.

Only Agilent authorized personnel are allowed to remove instrument covers. Always disconnect the power cables and any external circuits before removing the instrument cover.

Damage to the module can cause personal injury (for example electrical shock, intoxication).

Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel.

Toxic, flammable and hazardous solvents, samples and reagents present risks. The handling of solvents, samples and reagents can hold health and safety risks.

When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

Do not use solvents with an auto-ignition temperature below 200 °C (392 °F). Do not use solvents with a boiling point below 56 °C (133 °F).

Avoid high vapor concentrations. Keep the solvent temperature at least 40 K below the boiling point of the solvent used. This includes the solvent temperature in the sample compartment. For the solvents methanol and ethanol keep the solvent temperature at least 25 K below the boiling point.

Do not operate the instrument in an explosive atmosphere.

Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

Reduce the volume of substances to the minimum required for the analysis.

Never exceed the maximum permissible volume of solvents (8 L) in the solvent cabinet. Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for solvent cabinet.

Ground the waste container.

Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid.

To achieve maximal safety, regularly check the tubing for correct installation.

The following table explains the symbols found on the apparatus:

The following table explains additional symbols found on the apparatus:

A WARNING alerts you to situations that could cause physical injury or death. Do not proceed beyond a warning until you have fully understood and met the indicated conditions.

A CAUTION alerts you to situations that could cause loss of data, or damage of equipment. Do not proceed beyond a caution until you have fully understood and met the indicated conditions.

This product complies with the European WEEE Directive marking requirements. The affixed label (crossed-out wheeled bin symbol) indicates that you must not discard this electrical/electronic product in domestic household waste.

Do not dispose of in domestic household waste.

To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information.

The refrigerant HFC-134a is used only in the Agilent Infinity II Sample Cooler.

The physical properties of refrigerant HFC-134a are:

The physical properties of refrigerant R600a (isobutane) are:

Refrigerant HFC-134a is known as a safe refrigerant, however accidents can occur if it is handled incorrectly. For this reason, the following instructions must be observed:

Avoid contact with liquid refrigerant HFC-134a. At atmospheric pressure HFC-134a evaporates at approximately -26 °C and causes frost bite.

After skin contact, rinse the affected area with water.

After eye contact, rinse the eye(s) with plenty of water for at least 15 minutes and consult a doctor.

HFC-134a must not be allowed to escape in enclosed areas. Although HFC-134a is not toxic, there is a danger of suffocation as gaseous refrigerant is heavier than air.

Please observe the following first aid instructions. After inhalation, move the affected person to fresh air, keep him warm and allow him to rest. If necessary, he should be supplied with oxygen. If he has stopped breathing or is breathing erratically, he should be given artificial respiration. In the case of cardiac arrest, carry out heart massage. Send for a doctor immediately.

Moreover, it must be noted that HFC-134a must always be extracted from the system and collected. It must never be discharged into the atmosphere on environmental grounds (greenhouse effect).

General hazards and improper disposal: Improper disposal of the media and components used pollutes the environment.

The disposal or scrapping of the Sample Cooler or the Sample Thermostat must be carried out by a qualified disposal company.

All media must be disposed of in accordance with national and local regulations.

Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check http://www.agilent.com for more info.

Risk of fire or explosion applies to flammable refrigerant.

Dispose of properly in accordance with federal or local regulations. Flammable Refrigerant Used.

Do not dispose of in domestic household waste.

To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Test and Measurement: If test and measurement equipment is operated with unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

Manufacturer’s Declaration: This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991.

This product has a sound pressure emission (at the operator position) < 70 dB.

• Sound Pressure Lp < 70 dB (A)

• At Operator Position

• Normal Operation

• According to ISO 7779:1988/EN 27779/1991 (Type Test)

Observe the following recommendations on the use of solvents.

• Brown glass ware can avoid growth of algae.

• Avoid the use of the following steel-corrosive solvents:

• solutions of alkali halides and their respective acids (for example, lithium iodide, potassium chloride, and so on),

• high concentrations of inorganic acids like sulfuric acid and nitric acid, especially at higher temperatures (if your chromatography method allows, replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel),

• halogenated solvents or mixtures which form radicals and/or acids, for example: 2CHCl3 + O2→ 2COCl2 + 2HCl. This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol,

• chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropyl ether) should be filtered through dry aluminium oxide which adsorbs the peroxides,

• solvents containing strong complexing agents (e.g. EDTA),

• mixtures of carbon tetrachloride with 2-propanol or THF.

• Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF.

This installation procedure applies for capillaries and corresponding fittings used in modules delivered before January 2013.

The 1260 Infinity Bio-inert LC system uses PEEK capillaries that are cladded with stainless steel. These capillaries combine the high pressure stability of steel with the inertness of PEEK. They are used in the high pressure flow path after sample introduction (loop/needle seat capillary) through the thermostatted column compartment/heat exchangers to the column. Such capillaries need to be installed carefully in order to keep them tight without damaging them by over-tightening.

The installation consists of two steps. In the first step, the fitting is installed finger-tight without using tools. Finger-tight means that the fitting will grip and hold the capillary. This brings the fitting to the appropriate start position (marked as 0°) for the second step.

First Step: Finger-tight Fitting

1 Tighten the fitting using your fingers.

Finger-tight means that the fitting will grip and hold the capillary. This brings the fitting to the appropriate start position (marked as 0°) for the second step.

Second Step: Installation to Connector

In the second step, a wrench is used to rotate the fitting relative to the finger-tight position by a defined angle. For each of the cases mentioned (Hard or Soft Connectors), there is a recommended range in which the fitting is tight.

Staying below this range could create a leak, either a visible one or a micro-leak, potentially biasing measurement results. Exceeding the recommended range could damage the capillary.

Alternatively, a torque wrench may be used. The target torque for all connections is about 0.7 Nm. When using a torque wrench, read instructions for that tool carefully, as wrong handling may easily miss the correct torque.

Second Step: Installation to Hard Connectors

Use this procedure for hard connectors made from metal (titanium) or ceramics. In the system, these are connections to and from the analytical head of the autosampler (connections to injection valve and needle), and to a metal column.

First installation of a capillary to a hard connector:

1 When tightening a fitting for the first time, start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 135 – 180 °. Staying below 135 ° (grey arrow) will be insufficiently tight, more than 180° (red arrow) could damage the capillary.

Second and subsequent installations of a capillary to a hard connector:

1 When tightening the fitting for the second and subsequent times, again start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 90 – 135 °. Staying below 90 ° (grey arrow) could be insufficiently tight, more than 135 ° (red arrow) could damage the capillary.

Second Step: Installation to Soft Connectors

Use this procedure for soft connectors, which are typically made from PEEK. These are the following connections:

• to and from all bio-inert valves (injection valve in the autosampler and valves in the thermostatted column compartment and 1290 Infinity Valve Drive),

• bio-inert ZDV unions (detector flow cells, multi-draw upgrade kit, capillary to capillary connections, for example, for heat exchangers),

• to the autosampler needle and

• to PEEK columns (like many bio-inert columns).

For the installation of bio-inert ZDV unions, refer to the Technical Note “Installation of stainless steel cladded PEEK capillaries” (p/n G5611-90120).

First installation of a capillary to a soft connector:

1 When tightening a fitting for the first time, start from the finger-tight position (which does not necessarily need to be a vertical wrench position) and rotate the wrench by 180 – 210 °. Staying below 180° (grey arrow) will not be sufficiently tight, more than 210 ° (red arrow) could damage the capillary.

Second and subsequent installations of a capillary to a soft connector:

1 When tightening the fitting for the second and subsequent times, again start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 135 – 180 °. Staying below 135° (grey arrow) could be insufficiently tight enough, more than 180 ° (red arrow) could damage the capillary.

Yes, the required rotation angles for the second step (after finger-tightening) are summarized below:

Note: Staying below the recommended range may cause leaks (‘possibly leaky’). Exceeding the range (‘STOP’) may damage the capillary.

Potential damage of capillaries. Do not remove fittings from used capillaries.

To keep the flow path free of stainless steel, the front end of the capillary is made of PEEK. Under high pressure, or when in contact with some solvents, PEEK can expand to the shape of the connector where the capillary is installed. If the capillary is removed, this may become visible as a small step. In such cases, do not try to pull the fitting from the capillary, as this can destroy the front part of the capillary. Instead, carefully pull it to the rear. During installation of the capillary, the fitting will end up in the correct position.

Dual needle Sample Loops right:

NOTE: It is mandatory that the configuration of the dual needle system, especially sample loops, must match to the installed hardware to avoid damage to the system.

Dual needle Sample Loops left:

NOTE: It is mandatory that the configuration of the dual needle system, especially sample loops, must match to the installed hardware to avoid damage to the system.

Capillaries for the Dual Needle Option:

3Pos/6Port Peripheral Valve Dual Needle parts:

2Pos/8Port Injection Valve Dual Needle parts:

Needle Port Assembly parts:

Door Assy parts:

Accessory Kit parts:

Tools included in the standard accessory kit (Figure 65):

Tubing Connector Leak Kit (5067-6137):

Bottles:

Tubing Kit Sampler Standard parts:

NOTE: The components of the kit are not orderable separately.

Accessories not included in the kit, orderable separately:

Tubing Kit Sampler Multi-Wash parts (Kit: G4267-60081):

Multidraw Kit for InfinityLab Sampler parts (Kit: G7167-68711):

NOTE: At the moment, multidraw is only possible with the Standard Multisampler.

Large Volume Injection Kit for 1290 Infinity II Samplers parts (Kit: G4216-68711):

Bio-Inert Multi-Draw Kit parts (Multidraw upgrade kit (Bio-inert): G5667-68711):

Note: For bio-inert modules use bio-inert parts only!

Upgrade Kits:

NOTE: For instructions on how to install the Upgrade Kits, please refer to the respective Installation Notes:

• Agilent Infinity II Series Multi-wash Upgrade Kit Installation Note (G7167-90210)

• Dual-Needle Infinity II Upgrade Kit Installation Note (G7167-90220)

Leak System Parts (Drain management kit: G4267-68708):

Sample Thermostat parts (InfinityLab Sample Thermostat Upgrade Kit: G4761A):

NOTE: The Sample Thermostat contains flammable refrigerant R600a. Please check further details for installation.

Analog cables:

NOTE: Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

Remote cables:

NOTE: Never use cables other than the ones supplied by Agilent Technologies.

CAN cables:

NOTE: Never use cables other than the ones supplied by Agilent Technologies.

LAN cables:

NOTE: Never use cables other than the ones supplied by Agilent Technologies.

RS-232 cables (not for FUSION board):

NOTE: Never use cables other than the ones supplied by Agilent Technologies.

USB cables:

NOTE: Never use cables other than the ones supplied by Agilent Technologies.

Pinout for p/n 35900-60750:

Pinout for p/n 8120-1840:

Pinout for p/n 01046-60105:

Pinout for p/n 5188-8029 (D-Sub female 15way, user’s view to connector):

Pinout for p/n 5188-8045 (ERI to APG):

Pinout for p/n 5188-8057 (ERI to APG and RJ45):

Pinout for p/n 5061-3378:

Pinout for p/n 01046-60201:

Both ends of this cable provide a modular plug to be connected to Agilent modules CAN connectors.

CAN Cables:

LAN Cables:

Agilent Module to PC Cables:

To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket.

USB Cables:

This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules. Its properties are:

• the complete communication capabilities (CAN, LAN, USB and RS-232)

• memory management

• ability to update the firmware of the ‘main system’

Its properties are:

• the complete communication capabilities (CAN, LAN, USB and RS-232)

• memory management

• ability to update the firmware of the ‘resident system’

In addition the main system comprises the instrument functions that are divided into common functions like

• run synchronization through APG/ERI remote,

• error handling,

• diagnostic functions,

or module specific functions like

• internal events such as lamp control, filter movements,

• raw data collection and conversion to absorbance.

Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used).

Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761

For instructions on firmware updates refer to section Replacing Firmware in chapter “Maintenance” or use the documentation provided with the Firmware Update Tools.

The file naming conventions are: PPPP_RVVV_XXX.dlb, where

• PPPP is the product number, for example, 1315B for the G1315B DAD,

• R the firmware revision, for example, A for G1315B or B for the G1315C DAD,

• VVV is the revision number, for example 650 is revision 6.50,

• XXX is the build number of the firmware.

• The CAN bus is a serial bus with high-speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization.

• The ERI/REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shutdown, prepare, and so on.

• With the appropriate software, the LAN connector may be used to control the module from a computer through a LAN connection. This connector is activated and can be configured with the configuration switch.

• With the appropriate software, the USB connector may be used to control the module from a computer through a USB connection.

• The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a line frequency of 50 or 60 Hz. Maximum power consumption varies by module. There is no voltage selector on your module because the power supply has wide-ranging capability. There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply.

Serial Number Information 1200 Series and 1290 Infinity:

The serial number information on the instrument labels provide the following information:

Format: CCYWWSSSSS

• CC: country of manufacturing (DE = Germany, JP = Japan, CN = China)

• YWW: year and week of last major manufacturing change, e.g. 820 could be week 20 of 1998 or 2008

• SSSSS: real serial number

Serial Number Information 1260/1290 Infinity:

The serial number information on the instrument labels provide the following information:

Format: CCXZZ00000

• CC: Country of manufacturing (DE = Germany, JP = Japan, CN = China)

• X: Alphabetic character A-Z (used by manufacturing)

• ZZ: Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)

• 00000: Serial number

The Agilent InfinityLab LC Series modules provide the following interfaces:

The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on.

It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.

The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique).

The signal levels are defined as:

• standard TTL levels (0 V is logic true, + 5.0 V is false),

• fan-out is 10,

• input load is 2.2 kOhm against + 5.0 V, and

• output are open collector type, inputs/outputs (wired- or technique).

ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2)

ERI Description:

The ERI interface contains eight individual programmable input/output pins. In addition, it provides 24 V power and 5 V power and a serial data line to detect and recognize further add-ons that could be connected to this interface. This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc.

Pinout for ERI D-Sub female 15way (user’s view to connector):

IO (Input/Output) Lines:

• Eight generic bi-directional channels (input or output).

• Same as the APG Remote.

• Devices like valves, relays, ADCs, DACs, controllers can be supported/controlled.

1-Wire Data (Future Use):

This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required).

5V Distribution (Future Use):

• Available directly after turning on the hosting module (assures that the firmware can detect certain basic functionality of the device).

• For digital circuits or similar.

• Provides 500 mA maximum.

• Short-circuit proof with automatic switch off (by firmware).

24V Distribution (Future Use):

• Available by firmware command (defined turn on/off).

• For devices that need higher power

• Class 0: 0.5 A maximum (12 W)

• Class 1: 1.0 A maximum (24 W)

• Class 2: 2.0 A maximum (48 W)

• Class depends on hosting module’s internal power overhead.

• If a connected device requires more power the firmware detects this (overcurrent detection) and provides the information to the user interface.

• Fuse used for safety protection (on board).

• Short circuit will be detected through hardware.

USB (Universal Serial Bus) – replaces RS232, supports:

• a PC with control software (for example Agilent Lab Advisor)

• USB Flash Disk

All modules with FUSION electronics:

• Default is ALL switches DOWN (best settings).

• Default IP address for LAN 192.168.254.11

• For specific LAN modes switches 4-5 must be set as required.

• For boot resident/cold start modes switches 1+2 or 6 must be UP.

Notes:

¹ When selecting mode COM (Switch 1=0), settings are stored to non-volatile memory. When selecting mode TEST (Switch 1=1), COM settings are taken from non-volatile memory.

² n.a. = not assigned – Always keep these switches (2 and 3) on position ‘0’ (off).

³ Default IP Address is 192.168.254.11.

⁴ Host Name will be the MAC address.

The advantages of this packaging technology are:

• virtual elimination of fixing screws, bolts or ties, reducing the number of components and increasing the speed of assembly/disassembly,

• the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations,

• the plastic layers help cushion the electronic and mechanical parts from physical shock, and

• the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself.